PMID- 29509661 OWN - NLM STAT- MEDLINE DCOM- 20180905 LR - 20181114 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 19 IP - 3 DP - 2018 Mar 6 TI - Two New Cyathane Diterpenoids from Mycelial Cultures of the Medicinal Mushroom Hericium erinaceus and the Rare Species, Hericium flagellum. LID - 10.3390/ijms19030740 [doi] LID - 740 AB - Basidiomycetes of the genus Hericium are among the most praised medicinal and edible mushrooms, which are known to produce secondary metabolites with the potential to treat neurodegenerative diseases. This activity has been attributed to the discovery of various terpenoids that can stimulate the production of nerve growth factor (NGF) or (as established more recently) brain-derived neurotrophic factor (BDNF) in cell-based bioassays. The present study reports on the metabolite profiles of a Lion's Mane mushroom (Hericium erinaceus) strain and a strain of the rare species, Hericium flagellum (synonym H. alpestre). While we observed highly similar metabolite profiles between the two strains that were examined, we isolated two previously undescribed metabolites, given the trivial names erinacines Z1 and Z2. Their chemical structures were elucidated by means of nuclear magnetic resonance (NMR) spectroscopy and high resolution mass spectrometry. Along with six further, previously identified cyathane diterpenes, the novel erinacines were tested for neurotrophin inducing effects. We found that erinacines act on BDNF, which is a neurotrophic factor that has been reported recently by us to be induced by the corallocins, but as well on NGF expression, which is consistent with the literature. FAU - Rupcic, Zeljka AU - Rupcic Z AD - Department Microbial Drugs, Helmholtz Centre for Infection Research GmbH, Inhoffenstrasse 7, 38124 Braunschweig, Germany. zeljka.rupcic@helmholtz-hzi.com. AD - German Centre for Infection Research (DZIF), Partner Site Hannover-Braunschweig, 38124 Braunschweig, Germany. zeljka.rupcic@helmholtz-hzi.com. FAU - Rascher, Monique AU - Rascher M AD - Department Microbial Drugs, Helmholtz Centre for Infection Research GmbH, Inhoffenstrasse 7, 38124 Braunschweig, Germany. monique.rascher@helmholtz-hzi.de. AD - German Centre for Infection Research (DZIF), Partner Site Hannover-Braunschweig, 38124 Braunschweig, Germany. monique.rascher@helmholtz-hzi.de. AD - Zoological Institute, Technical University of Braunschweig, Spielmannstrasse 7, 38106 Braunschweig, Germany. monique.rascher@helmholtz-hzi.de. FAU - Kanaki, Sae AU - Kanaki S AD - Department Microbial Drugs, Helmholtz Centre for Infection Research GmbH, Inhoffenstrasse 7, 38124 Braunschweig, Germany. t416010@st.pu-toyama.ac.jp. AD - Toyama Prefectural University, 5180 Kurokawa, Imizu-shi, Toyama 939-0398, Japan. t416010@st.pu-toyama.ac.jp. FAU - Koster, Reinhard W AU - Koster RW AUID- ORCID: 0000-0001-6593-8196 AD - Zoological Institute, Technical University of Braunschweig, Spielmannstrasse 7, 38106 Braunschweig, Germany. r.koester@tu-braunschweig.de. FAU - Stadler, Marc AU - Stadler M AUID- ORCID: 0000-0002-7284-8671 AD - Department Microbial Drugs, Helmholtz Centre for Infection Research GmbH, Inhoffenstrasse 7, 38124 Braunschweig, Germany. marc.stadler@helmholtz-hzi.de. AD - German Centre for Infection Research (DZIF), Partner Site Hannover-Braunschweig, 38124 Braunschweig, Germany. marc.stadler@helmholtz-hzi.de. FAU - Wittstein, Kathrin AU - Wittstein K AD - Department Microbial Drugs, Helmholtz Centre for Infection Research GmbH, Inhoffenstrasse 7, 38124 Braunschweig, Germany. kathrin.wittstein@helmholtz-hzi.de. AD - German Centre for Infection Research (DZIF), Partner Site Hannover-Braunschweig, 38124 Braunschweig, Germany. kathrin.wittstein@helmholtz-hzi.de. LA - eng PT - Journal Article DEP - 20180306 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 RN - 0 (Biological Products) RN - 0 (Brain-Derived Neurotrophic Factor) RN - 0 (Diterpenes) RN - 0 (erinacine Z) SB - IM MH - Animals MH - Basidiomycota/*chemistry MH - Biological Products/*chemistry/pharmacology MH - Brain-Derived Neurotrophic Factor/metabolism MH - Cell Differentiation/drug effects MH - Diterpenes/*chemistry/pharmacology MH - Humans MH - Mycelium/chemistry MH - PC12 Cells MH - Rats PMC - PMC5877601 OTO - NOTNLM OT - Basidiomycota OT - fermentation OT - fungal metabolites OT - natural products OT - neurotrophic activity COIS- The authors declare no conflict of interest. EDAT- 2018/03/07 06:00 MHDA- 2018/09/06 06:00 PMCR- 2018/03/01 CRDT- 2018/03/07 06:00 PHST- 2018/01/31 00:00 [received] PHST- 2018/02/23 00:00 [revised] PHST- 2018/03/02 00:00 [accepted] PHST- 2018/03/07 06:00 [entrez] PHST- 2018/03/07 06:00 [pubmed] PHST- 2018/09/06 06:00 [medline] PHST- 2018/03/01 00:00 [pmc-release] AID - ijms19030740 [pii] AID - ijms-19-00740 [pii] AID - 10.3390/ijms19030740 [doi] PST - epublish SO - Int J Mol Sci. 2018 Mar 6;19(3):740. doi: 10.3390/ijms19030740.