PMID- 29510210
OWN - NLM
STAT- MEDLINE
DCOM- 20190311
LR  - 20190311
IS  - 1873-7544 (Electronic)
IS  - 0306-4522 (Linking)
VI  - 377
DP  - 2018 May 1
TI  - 3,4-Methylenedioxymethamphetamine (MDMA) Alters Synaptic Dopamine Release in the 
      Dorsal Striatum Through Nicotinic Receptors and DAT Inhibition.
PG  - 69-76
LID - S0306-4522(18)30162-3 [pii]
LID - 10.1016/j.neuroscience.2018.02.037 [doi]
AB  - An increase of extracellular dopamine (DA) has been implicated in the 
      psychostimulant properties of 3,4-methylenedioxymethamphetamine (MDMA). Although 
      this drug has been reported to affect the DA uptake transporter (DAT), it might 
      activate other mechanisms to regulate the outflow of DA in the brain. Our aim was 
      to examine the overall effects of MDMA on the release of DA in the striatum. We 
      studied the effect of MDMA on stimulus-evoked synaptic DA release in dorsal 
      striatal slices of mice using in vitro amperometric techniques. We also tested 
      the effects of MDMA on the nicotine-induced responses in substantia nigra pars 
      compacta (SNpc) neurons using intracellular electrophysiological recordings. MDMA 
      (1-30 microM) depressed the amplitude and prolonged the decay-time of synaptic DA 
      release in the striatum. Interestingly, in the presence of the broad nicotinic 
      receptor antagonist mecamylamine, and the more selective alpha(4)beta(2) antagonist 
      dihydrobetaerythroidine (DHbetaE), MDMA enhanced both peak and duration of DA release. 
      A similar effect was found on cocaine-insensitive (DAT-CI) mice slices. 
      Concentrations of MDMA higher than 100 microM enhanced striatal DA outflow that was 
      in turn, reduced by cocaine. Electrophysiological recordings of dopaminergic 
      neurons in SNpc showed that MDMA depressed the effects of nicotine. Our data are 
      consistent with a prevalent MDMA-induced inhibition of the synaptic release of DA 
      in the dorsal striatum mediated by an interaction with nicotinic receptors. This 
      drug also blocks DAT acting on a different site from cocaine and, at higher 
      concentrations, has amphetamine-like releasing properties.
CI  - Copyright (c) 2018 IBRO. Published by Elsevier Ltd. All rights reserved.
FAU - Rizzo, Francesca Romana
AU  - Rizzo FR
AD  - University of Rome 'Tor Vergata', 00133 Rome, Italy.
FAU - Federici, Mauro
AU  - Federici M
AD  - IRCSS Fondazione Santa Lucia, 00143 Rome, Italy.
FAU - Mercuri, Nicola Biagio
AU  - Mercuri NB
AD  - University of Rome 'Tor Vergata', 00133 Rome, Italy; IRCSS Fondazione Santa 
      Lucia, 00143 Rome, Italy. Electronic address: mercurin@med.uniroma2.it.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180303
PL  - United States
TA  - Neuroscience
JT  - Neuroscience
JID - 7605074
RN  - 0 (Dopamine Plasma Membrane Transport Proteins)
RN  - 0 (Hallucinogens)
RN  - 0 (Receptors, Nicotinic)
RN  - KE1SEN21RM (N-Methyl-3,4-methylenedioxyamphetamine)
RN  - VTD58H1Z2X (Dopamine)
SB  - IM
MH  - Animals
MH  - Corpus Striatum/*drug effects/metabolism
MH  - Dopamine/*metabolism
MH  - Dopamine Plasma Membrane Transport Proteins/*antagonists & 
      inhibitors/genetics/metabolism
MH  - Dose-Response Relationship, Drug
MH  - Hallucinogens/*pharmacology
MH  - Mice, Inbred C57BL
MH  - Mice, Transgenic
MH  - N-Methyl-3,4-methylenedioxyamphetamine/*pharmacology
MH  - Receptors, Nicotinic/*metabolism
MH  - Synapses/drug effects/metabolism
MH  - Tissue Culture Techniques
OTO - NOTNLM
OT  - 3,4-methylenedioxymethamphetamine (MDMA)
OT  - DA transporter
OT  - cocaine
OT  - dopamine
OT  - nicotinic cholinergic receptors
EDAT- 2018/03/07 06:00
MHDA- 2019/03/12 06:00
CRDT- 2018/03/07 06:00
PHST- 2017/09/22 00:00 [received]
PHST- 2018/02/14 00:00 [revised]
PHST- 2018/02/22 00:00 [accepted]
PHST- 2018/03/07 06:00 [pubmed]
PHST- 2019/03/12 06:00 [medline]
PHST- 2018/03/07 06:00 [entrez]
AID - S0306-4522(18)30162-3 [pii]
AID - 10.1016/j.neuroscience.2018.02.037 [doi]
PST - ppublish
SO  - Neuroscience. 2018 May 1;377:69-76. doi: 10.1016/j.neuroscience.2018.02.037. Epub 
      2018 Mar 3.