PMID- 29511223
OWN - NLM
STAT- MEDLINE
DCOM- 20190920
LR  - 20240327
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 8
IP  - 1
DP  - 2018 Mar 6
TI  - Rice intermediate filament, OsIF, stabilizes photosynthetic machinery and yield 
      under salinity and heat stress.
PG  - 4072
LID - 10.1038/s41598-018-22131-0 [doi]
LID - 4072
AB  - Cytoskeleton plays a vital role in stress tolerance; however, involvement of 
      intermediate filaments (IFs) in such a response remains elusive in crop plants. 
      This study provides clear evidence about the unique involvement of IFs in 
      cellular protection against abiotic stress in rice. Transcript abundance of Oryza 
      sativa intermediate filament (OsIF) encoding gene showed 2-10 fold up-regulation 
      under different abiotic stress. Overexpression of OsIF in transgenic rice 
      enhanced tolerance to salinity and heat stress, while its knock-down (KD) 
      rendered plants more sensitive thereby indicating the role of IFs in promoting 
      survival under stress. Seeds of OsIF overexpression rice germinated normally in 
      the presence of high salt, showed better growth, maintained chloroplast 
      ultrastructure and favourable K(+)/Na(+) ratio than the wild type (WT) and KD 
      plants. Analysis of photosynthesis and chlorophyll a fluorescence data suggested 
      better performance of both photosystem I and II in the OsIF overexpression rice 
      under salinity stress as compared to the WT and KD. Under salinity and high 
      temperature stress, OsIF overexpressing plants could maintain significantly high 
      yield, while the WT and KD plants could not. Further, metabolite profiling 
      revealed a 2-4 fold higher accumulation of proline and trehalose in OsIF 
      overexpressing rice than WT, under salinity stress.
FAU - Soda, Neelam
AU  - Soda N
AD  - Stress Physiology and Molecular Biology Laboratory, School of Life Sciences, 
      Jawaharlal Nehru University, New Delhi, 110067, India.
FAU - Gupta, Brijesh K
AU  - Gupta BK
AD  - Plant Stress Biology, International Centre for Genetic Engineering and 
      Biotechnology, Aruna Asaf Ali Marg, New Delhi, 110067, India.
FAU - Anwar, Khalid
AU  - Anwar K
AD  - Stress Physiology and Molecular Biology Laboratory, School of Life Sciences, 
      Jawaharlal Nehru University, New Delhi, 110067, India.
FAU - Sharan, Ashutosh
AU  - Sharan A
AD  - Stress Physiology and Molecular Biology Laboratory, School of Life Sciences, 
      Jawaharlal Nehru University, New Delhi, 110067, India.
FAU - Govindjee
AU  - Govindjee
AD  - Department of Biochemistry, Center of Biophysics & Quantitative Biology, 
      University of Illinois at Urbana-Champaign, 265 Morrill Hall, 505 South Goodwin 
      Av, Urbana, IL, 61801-3707, USA.
FAU - Singla-Pareek, Sneh L
AU  - Singla-Pareek SL
AD  - Plant Stress Biology, International Centre for Genetic Engineering and 
      Biotechnology, Aruna Asaf Ali Marg, New Delhi, 110067, India.
FAU - Pareek, Ashwani
AU  - Pareek A
AUID- ORCID: 0000-0002-2923-0681
AD  - Stress Physiology and Molecular Biology Laboratory, School of Life Sciences, 
      Jawaharlal Nehru University, New Delhi, 110067, India. ashwanip@mail.jnu.ac.in.
AD  - The UWA Institute of Agriculture, School of Agriculture and Environment, The 
      University of Western Australia, Perth, WA, Australia. ashwanip@mail.jnu.ac.in.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180306
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (RNA, Messenger)
RN  - 9DLQ4CIU6V (Proline)
RN  - B8WCK70T7I (Trehalose)
SB  - IM
EIN - Sci Rep. 2018 May 11;8(1):7621. PMID: 29752473
EIN - Sci Rep. 2019 Jul 24;9(1):11015. PMID: 31337776
MH  - Gene Expression
MH  - Gene Expression Profiling
MH  - Gene Expression Regulation, Plant
MH  - Gene Knockdown Techniques
MH  - *Heat-Shock Response
MH  - Intermediate Filaments/genetics/*metabolism
MH  - Metabolomics
MH  - Oryza/drug effects/genetics/*physiology/radiation effects
MH  - *Photosynthesis
MH  - Proline/analysis
MH  - RNA, Messenger/analysis
MH  - *Salinity
MH  - *Stress, Physiological
MH  - Trehalose/analysis
PMC - PMC5840354
COIS- The authors declare no competing interests.
EDAT- 2018/03/08 06:00
MHDA- 2019/09/21 06:00
PMCR- 2018/03/06
CRDT- 2018/03/08 06:00
PHST- 2017/08/25 00:00 [received]
PHST- 2018/02/19 00:00 [accepted]
PHST- 2018/03/08 06:00 [entrez]
PHST- 2018/03/08 06:00 [pubmed]
PHST- 2019/09/21 06:00 [medline]
PHST- 2018/03/06 00:00 [pmc-release]
AID - 10.1038/s41598-018-22131-0 [pii]
AID - 22131 [pii]
AID - 10.1038/s41598-018-22131-0 [doi]
PST - epublish
SO  - Sci Rep. 2018 Mar 6;8(1):4072. doi: 10.1038/s41598-018-22131-0.