PMID- 29512143
OWN - NLM
STAT- MEDLINE
DCOM- 20190401
LR  - 20200108
IS  - 1934-368X (Electronic)
IS  - 1934-3671 (Linking)
VI  - 120
DP  - 2018 Feb 21
TI  - Isolation of Conventional Murine Lung Dendritic Cell Subsets.
PG  - 3.7B.1-3.7B.16
LID - 10.1002/cpim.39 [doi]
AB  - The lungs are continuously exposed to environmental threats, requiring an 
      adequate and stringent immune response of a heterogeneous set of effector cells. 
      Dendritic cells (DCs) form a dense network in the respiratory mucosa and act as 
      the central regulators of the different components of this response, both sensing 
      the nature of the threats and precisely coordinating the effector mechanisms best 
      suited for overcoming it. The DCs are classically subdivided in two main groups, 
      plasmacytoid DCs (pDCs) and conventional DCs (cDCs), the latter being further 
      subdivided into cDC1s and cDC2s based on ontogeny and their distinct 
      non-redundant functions. This protocol provides different enrichment methods and 
      represents an up-to-date, universal framework that uses a minimal set of highly 
      specific lineage markers to discriminate and sort pure cDC subsets from the 
      murine lung but also across tissues and species which is an added value in intra- 
      and interspecies comparative research. (c) 2018 by John Wiley & Sons, Inc.
CI  - Copyright (c) 2018 John Wiley & Sons, Inc.
FAU - Bosteels, Cedric
AU  - Bosteels C
AD  - Laboratory of Immunoregulation and Mucosal Immunology, VIB Center for 
      Inflammation Research, Ghent, Belgium.
AD  - Department of Internal Medicine, Ghent University, Ghent, Belgium.
FAU - Lambrecht, Bart N
AU  - Lambrecht BN
AD  - Laboratory of Immunoregulation and Mucosal Immunology, VIB Center for 
      Inflammation Research, Ghent, Belgium.
AD  - Department of Internal Medicine, Ghent University, Ghent, Belgium.
FAU - Hammad, Hamida
AU  - Hammad H
AD  - Laboratory of Immunoregulation and Mucosal Immunology, VIB Center for 
      Inflammation Research, Ghent, Belgium.
AD  - Department of Internal Medicine, Ghent University, Ghent, Belgium.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180221
PL  - United States
TA  - Curr Protoc Immunol
JT  - Current protocols in immunology
JID - 9101651
RN  - 0 (CD11c Antigen)
SB  - IM
MH  - Animals
MH  - CD11c Antigen
MH  - Cell Separation
MH  - Dendritic Cells/*cytology
MH  - Lung/cytology
MH  - Mice
MH  - Staining and Labeling
OTO - NOTNLM
OT  - dendritic cell
OT  - isolation
OT  - lung
OT  - mouse
OT  - subtypes
EDAT- 2018/03/08 06:00
MHDA- 2019/04/02 06:00
CRDT- 2018/03/08 06:00
PHST- 2018/03/08 06:00 [entrez]
PHST- 2018/03/08 06:00 [pubmed]
PHST- 2019/04/02 06:00 [medline]
AID - 10.1002/cpim.39 [doi]
PST - epublish
SO  - Curr Protoc Immunol. 2018 Feb 21;120:3.7B.1-3.7B.16. doi: 10.1002/cpim.39.