PMID- 29512704
OWN - NLM
STAT- MEDLINE
DCOM- 20180910
LR  - 20211204
IS  - 1791-244X (Electronic)
IS  - 1107-3756 (Print)
IS  - 1107-3756 (Linking)
VI  - 41
IP  - 6
DP  - 2018 Jun
TI  - Intravenous administration of DPSCs and BDNF improves neurological performance in 
      rats with focal cerebral ischemia.
PG  - 3185-3194
LID - 10.3892/ijmm.2018.3517 [doi]
AB  - Dental pulp stem cells (DPSCs) are considered as an ideal stem cell source for 
      the treatment of neurological diseases. In this study, we evaluated the 
      therapeutic potency of DPSCs and brain-derived neurotrophic factor (BDNF) in 
      focal cerebral ischemia using animal models. Following middle cerebral artery 
      occlusion (MCAO), rats were randomized into four groups: the BDNF, DPSCs, 
      DPSCs+BDNF and the controls injected with saline. DPSCs were transplanted and 
      BDNF was injected into the DPSCs+BDNF group via the tail vein. The fate of the 
      transplanted DPSCs in rat brains was evaluated using immunofluorescence, 
      immunohistochemistry, western blot analysis and reverse transcription-polymerase 
      chain reaction (RT-PCR). Adhesive removal tests and the modified neurological 
      severity scores were used to estimate the restoration of neurological function. 
      Proliferation of intravenously transplanted DPSCs was observed in the peripheral 
      ischemic regions of the MCAO models. A green fluorescent dye PKH67 was used to 
      label cells. PKH67-labeled DPSCs were co-localized with neuronal cell markers and 
      4',6-diamidino‑2-phenylindole (DAPI). DPSC transplantation combined with BDNF 
      induced the expression of neural differentiation markers such as nestin, 
      doublecortin (DCX) and neuronal specific filament (NF-H), suggesting that BDNF 
      enhances the survival of DPSCs and differentiation into neuronal cells. Treatment 
      with DPSCs combined with BDNF promoted the recovery of neurological function more 
      effectively compared with BDNF injection or DPSC transplantation alone. In 
      conclusion, treatment with DPSCs combined with BDNF enhances neurological 
      recovery after stroke suggesting a novel therapeutic strategy against cerebral 
      ischemia.
FAU - Zhang, Xuemei
AU  - Zhang X
AD  - Department of Neurology, The Second Affiliated Hospital of Harbin Medical 
      University, Harbin, Heilongjiang 150086, P.R. China.
FAU - Zhou, Yinglian
AU  - Zhou Y
AD  - Department of Neurology, The Second Affiliated Hospital of Harbin Medical 
      University, Harbin, Heilongjiang 150086, P.R. China.
FAU - Li, Hulun
AU  - Li H
AD  - Department of Neurobiology, Neurobiology Key Laboratory, Harbin Medical 
      University, Harbin, Heilongjiang 150086, P.R. China.
FAU - Wang, Rui
AU  - Wang R
AD  - Department of Neurology, The Second Affiliated Hospital of Harbin Medical 
      University, Harbin, Heilongjiang 150086, P.R. China.
FAU - Yang, Dan
AU  - Yang D
AD  - Department of Neurology, The Second Affiliated Hospital of Harbin Medical 
      University, Harbin, Heilongjiang 150086, P.R. China.
FAU - Li, Bing
AU  - Li B
AD  - Department of Neurology, The Second Affiliated Hospital of Harbin Medical 
      University, Harbin, Heilongjiang 150086, P.R. China.
FAU - Fu, Jin
AU  - Fu J
AD  - Department of Neurology, The Second Affiliated Hospital of Harbin Medical 
      University, Harbin, Heilongjiang 150086, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20180228
PL  - Greece
TA  - Int J Mol Med
JT  - International journal of molecular medicine
JID - 9810955
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Dcx protein, rat)
RN  - 0 (Doublecortin Protein)
SB  - IM
MH  - Administration, Intravenous
MH  - Animals
MH  - Brain Ischemia/*metabolism/*therapy
MH  - Brain-Derived Neurotrophic Factor/genetics/*metabolism
MH  - Dental Pulp/*cytology
MH  - Doublecortin Protein
MH  - Male
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Stem Cell Transplantation/methods
MH  - Stem Cells/*cytology/physiology
PMC - PMC5881652
EDAT- 2018/03/08 06:00
MHDA- 2018/09/11 06:00
PMCR- 2018/02/28
CRDT- 2018/03/08 06:00
PHST- 2016/12/07 00:00 [received]
PHST- 2018/02/12 00:00 [accepted]
PHST- 2018/03/08 06:00 [pubmed]
PHST- 2018/09/11 06:00 [medline]
PHST- 2018/03/08 06:00 [entrez]
PHST- 2018/02/28 00:00 [pmc-release]
AID - ijmm-41-06-3185 [pii]
AID - 10.3892/ijmm.2018.3517 [doi]
PST - ppublish
SO  - Int J Mol Med. 2018 Jun;41(6):3185-3194. doi: 10.3892/ijmm.2018.3517. Epub 2018 
      Feb 28.