PMID- 29513250 OWN - NLM STAT- MEDLINE DCOM- 20180919 LR - 20180919 IS - 2299-2847 (Electronic) IS - 0032-373X (Linking) VI - 90 IP - 1 DP - 2018 Feb 28 TI - Role of crossmatch testing when Luminex-SAB is negative in renal transplantation. PG - 41-46 LID - 10.5604/01.3001.0011.5959 [doi] AB - The human leukocyte antigen (HLA) system plays an important role in the acceptance of renal graft. Long and better graft survival has been reported in patients with HLA-identical siblings and a nonreactive cytotoxicity assay (CDC). New methods of HLA-typing and anti-HLA antibody detection techniques such as flow cytometry, solid-phase immunoassays, or antigen bead assays have further improved the outcomes of renal transplant recipients. In the present review, the explicit details of these methodologies are discussed in detail. FAU - Jayant, Kumar AU - Jayant K AD - Department of Surgery and Cancer; Faculty of Medicine, Hammersmith Hospital, Imperial College London, UK. FAU - Reccia, Isabella AU - Reccia I AD - Department of Surgery and Cancer; Faculty of Medicine, Hammersmith Hospital, Imperial College London, UK. FAU - Julie, Bridson M AU - Julie BM AD - Faculty of Health and Sciences, Institute of Learning and Teaching, University of Liverpool, Liverpool L693BX, United Kingdom. FAU - Sharma, Ajay AU - Sharma A AD - Faculty of Health and Sciences, Institute of Learning and Teaching, University of Liverpool, Liverpool L693BX, United Kingdom. FAU - Halawa, Ahmed AU - Halawa A AD - Faculty of Health and Sciences, Institute of Learning and Teaching, University of Liverpool, Liverpool L693BX, United Kingdom. LA - eng PT - Journal Article PT - Review PL - Poland TA - Pol Przegl Chir JT - Polski przeglad chirurgiczny JID - 0376426 RN - 0 (HLA Antigens) RN - 0 (Isoantibodies) SB - IM MH - Antibody Specificity MH - Flow Cytometry MH - Graft Rejection/immunology MH - Graft Survival/*immunology MH - HLA Antigens/*immunology MH - Histocompatibility/immunology MH - Histocompatibility Testing/*methods MH - Humans MH - Isoantibodies/*immunology MH - *Kidney Transplantation OTO - NOTNLM OT - HLA-typing OT - anti-HLA antibody OT - human leukocyte antigen OT - renal transplant EDAT- 2018/03/08 06:00 MHDA- 2018/09/20 06:00 CRDT- 2018/03/08 06:00 PHST- 2018/03/08 06:00 [entrez] PHST- 2018/03/08 06:00 [pubmed] PHST- 2018/09/20 06:00 [medline] AID - 01.3001.0011.5959 [pii] AID - 10.5604/01.3001.0011.5959 [doi] PST - ppublish SO - Pol Przegl Chir. 2018 Feb 28;90(1):41-46. doi: 10.5604/01.3001.0011.5959.