PMID- 29513745
OWN - NLM
STAT- MEDLINE
DCOM- 20180710
LR  - 20201214
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 13
IP  - 3
DP  - 2018
TI  - Nicotinic acetylcholine receptor (CHRN) expression and function in cultured human 
      adult fungiform (HBO) taste cells.
PG  - e0194089
LID - 10.1371/journal.pone.0194089 [doi]
LID - e0194089
AB  - In rodents, CHRNs are involved in bitter taste transduction of nicotine and 
      ethanol. Currently, it is not clear if CHRNs are expressed in human taste cells 
      and if they play a role in transducing the bitter taste of nicotine and ethanol 
      or in the synthesis and release of neurohumoral peptides. Accordingly, we 
      investigated the expression and functional role of CHRNs in HBO cells. Using 
      molecular techniques, we demonstrate that a subset of HBO cells express CHRNs 
      that also co-express TRPM5, T1R3 or T2R38. Exposing HBO cells to nicotine or 
      ethanol acutely or to nicotine chronically induced a differential increase in the 
      expression of CHRN mRNA and protein in a dose- and time-dependent manner. Acutely 
      exposing HBO cells to a mixture containing nicotine plus ethanol induced a 
      smaller increase in CHRN mRNAs relative to nicotine or ethanol treatment alone. A 
      subset of HBO cells responded to nicotine, acetylcholine and ATP with a transient 
      increase in [Ca2+]i. Nicotine effects on [Ca2+]i were mecamylamine sensitive. 
      Brain-derived neurotrophic factor (BDNF) protein was detected in HBO cells using 
      ELISA. Acute nicotine exposure decreased BDNF in HBO cells and increased BDNF 
      release in the medium. CHRNs were also detected in HEK293 cells by RT-PCR. Unlike 
      HBO cells, CHRNs were localized in most of HEK293 cells and majority of HEK293 
      cells responded to nicotine and ethanol stimulation with a transient increase in 
      [Ca2+]i. BDNF levels in HEK293 cells were significantly higher than in HBO cells 
      but the nicotine induced release of BDNF in the media was a fraction of the BDNF 
      cellular content. We conclude that CHRNs are expressed in TRPM5 positive HBO 
      cells. CHRN mRNA expression is modulated by exposure to nicotine and ethanol in a 
      dose- and time-dependent manner. Nicotine induces the synthesis and release of 
      BDNF in HBO cells.
FAU - Qian, Jie
AU  - Qian J
AD  - Department of Physiology and Biophysics, Virginia Commonwealth University, 
      Richmond, VA, United States of America.
FAU - Mummalaneni, Shobha
AU  - Mummalaneni S
AD  - Department of Physiology and Biophysics, Virginia Commonwealth University, 
      Richmond, VA, United States of America.
FAU - Larsen, James
AU  - Larsen J
AD  - Department of Physiology and Biophysics, Virginia Commonwealth University, 
      Richmond, VA, United States of America.
FAU - Grider, John R
AU  - Grider JR
AD  - Department of Physiology and Biophysics, Virginia Commonwealth University, 
      Richmond, VA, United States of America.
FAU - Spielman, Andrew I
AU  - Spielman AI
AD  - NYU College of Dentistry, New York, NY, United States of America.
FAU - Ozdener, Mehmet Hakan
AU  - Ozdener MH
AD  - Monell Chemical Senses Center, Philadelphia, PA, United States of America.
FAU - Lyall, Vijay
AU  - Lyall V
AUID- ORCID: 0000-0001-6567-5023
AD  - Department of Physiology and Biophysics, Virginia Commonwealth University, 
      Richmond, VA, United States of America.
LA  - eng
GR  - P30 DC011735/DC/NIDCD NIH HHS/United States
GR  - 5P30NS047463/NH/NIH HHS/United States
GR  - R01 DK034153/DK/NIDDK NIH HHS/United States
GR  - P30 NS047463/NS/NINDS NIH HHS/United States
GR  - R01 DC011569/DC/NIDCD NIH HHS/United States
GR  - DK34153/NH/NIH HHS/United States
GR  - DC-011569/NH/NIH HHS/United States
GR  - R56 DK034153/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20180307
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Protein Subunits)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Nicotinic)
RN  - 3K9958V90M (Ethanol)
RN  - 6M3C89ZY6R (Nicotine)
RN  - 7171WSG8A2 (BDNF protein, human)
SB  - IM
MH  - Adult
MH  - Brain-Derived Neurotrophic Factor/biosynthesis/genetics
MH  - Calcium Signaling/drug effects
MH  - Cells, Cultured
MH  - Ethanol/pharmacology
MH  - Gene Expression Regulation
MH  - HEK293 Cells
MH  - Humans
MH  - Nicotine/pharmacology
MH  - Protein Subunits
MH  - RNA, Messenger/biosynthesis/genetics
MH  - Receptors, Nicotinic/*biosynthesis/genetics
MH  - Taste Buds/*metabolism
PMC - PMC5841828
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2018/03/08 06:00
MHDA- 2018/07/11 06:00
PMCR- 2018/03/07
CRDT- 2018/03/08 06:00
PHST- 2017/12/06 00:00 [received]
PHST- 2018/02/25 00:00 [accepted]
PHST- 2018/03/08 06:00 [entrez]
PHST- 2018/03/08 06:00 [pubmed]
PHST- 2018/07/11 06:00 [medline]
PHST- 2018/03/07 00:00 [pmc-release]
AID - PONE-D-17-42841 [pii]
AID - 10.1371/journal.pone.0194089 [doi]
PST - epublish
SO  - PLoS One. 2018 Mar 7;13(3):e0194089. doi: 10.1371/journal.pone.0194089. 
      eCollection 2018.