PMID- 29513845
OWN - NLM
STAT- MEDLINE
DCOM- 20180419
LR  - 20180419
IS  - 1678-9849 (Electronic)
IS  - 0037-8682 (Linking)
VI  - 51
IP  - 1
DP  - 2018 Jan-Feb
TI  - Development and characterization of a packaging cell line for pseudo-infectious 
      yellow fever virus particle generation.
PG  - 66-70
LID - S0037-86822018000100066 [pii]
LID - 10.1590/0037-8682-0220-2017 [doi]
AB  - INTRODUCTION: Pseudo-infectious yellow fever viral particles (YFV-PIVs) have been 
      used to study vaccines and viral packaging. Here, we report the development of a 
      packaging cell line, which expresses the YFV prM/E proteins. METHODS: HEK293 
      cells were transfected with YFV prM/E and C (84 nt) genes to generate 
      HEK293-YFV-PrM/E-opt. The cells were evaluated for their ability to express the 
      heterologous proteins and to package the replicon repYFV-17D-LucIRES, generating 
      YFV-PIVs. RESULTS: The expression of prM/E proteins was confirmed, and the cell 
      line trans-packaged the replicon for recovery of a reporter for the YFV-PIVs. 
      CONCLUSIONS: HEK293-YFV-prM/E-opt trans-packaging capacity demonstrates its 
      possible biotechnology application.
FAU - Queiroz, Sabrina Ribeiro de Almeida
AU  - Queiroz SRA
AD  - Departamento de Virologia e Terapia Experimental, Instituto Aggeu Magalhaes, 
      Fundacao Oswaldo Cruz, Recife, PE, Brasil.
FAU - Silva Junior, Jose Valter Joaquim
AU  - Silva Junior JVJ
AD  - Departamento de Virologia e Terapia Experimental, Instituto Aggeu Magalhaes, 
      Fundacao Oswaldo Cruz, Recife, PE, Brasil.
FAU - Silva, Andrea Nazare Monteiro Rangel da
AU  - Silva ANMRD
AD  - Laboratorio de Virologia, Instituto de Ciencias Biologicas, Universidade Federal 
      do Para-UFPA, Belem, Para, Brasil.
FAU - Carvalho, Amanda Gomes de Oliveira
AU  - Carvalho AGO
AD  - Departamento de Virologia e Terapia Experimental, Instituto Aggeu Magalhaes, 
      Fundacao Oswaldo Cruz, Recife, PE, Brasil.
FAU - Santos, Jefferson Jose da Silva
AU  - Santos JJDS
AD  - Department of Population Health, Poultry Diagnostic and Research Center, 
      University of Georgia, Athens, USA.
FAU - Gil, Laura Helena Vega Gonzales
AU  - Gil LHVG
AD  - Departamento de Virologia e Terapia Experimental, Instituto Aggeu Magalhaes, 
      Fundacao Oswaldo Cruz, Recife, PE, Brasil.
LA  - eng
PT  - Journal Article
PL  - Brazil
TA  - Rev Soc Bras Med Trop
JT  - Revista da Sociedade Brasileira de Medicina Tropical
JID - 7507456
RN  - 0 (Vaccines, Virus-Like Particle)
RN  - 147336-22-9 (Green Fluorescent Proteins)
SB  - IM
MH  - Flow Cytometry
MH  - Fluorescent Antibody Technique, Indirect
MH  - Green Fluorescent Proteins
MH  - HEK293 Cells
MH  - Humans
MH  - Vaccines, Virus-Like Particle/genetics/*immunology
MH  - Virus Assembly/genetics/*immunology
MH  - Virus Replication/genetics/*immunology
MH  - Yellow fever virus/genetics/*immunology
EDAT- 2018/03/08 06:00
MHDA- 2018/04/20 06:00
CRDT- 2018/03/08 06:00
PHST- 2017/06/01 00:00 [received]
PHST- 2017/09/12 00:00 [accepted]
PHST- 2018/03/08 06:00 [entrez]
PHST- 2018/03/08 06:00 [pubmed]
PHST- 2018/04/20 06:00 [medline]
AID - S0037-86822018000100066 [pii]
AID - 10.1590/0037-8682-0220-2017 [doi]
PST - ppublish
SO  - Rev Soc Bras Med Trop. 2018 Jan-Feb;51(1):66-70. doi: 
      10.1590/0037-8682-0220-2017.