PMID- 29514172
OWN - NLM
STAT- MEDLINE
DCOM- 20181029
LR  - 20220408
IS  - 1745-7270 (Electronic)
IS  - 1672-9145 (Linking)
VI  - 50
IP  - 4
DP  - 2018 Apr 1
TI  - Myeloid-derived suppressor cells suppress CD4+ T cell activity and prevent the 
      development of type 2 diabetes.
PG  - 362-369
LID - 10.1093/abbs/gmy014 [doi]
AB  - CD4+ T cells play an important role in the progression of type 2 diabetes 
      mellitus (T2DM). It is known that T cell responses can be suppressed by 
      myeloid-derived suppressor cells (MDSCs). In this study, we aimed to explore the 
      potential role of MDSCs in the progression of T2DM, and to examine whether the 
      underlying mechanism was associated with CD4+ T cells. Peripheral blood samples 
      were obtained from T2DM patients and healthy controls, as well as C57BL6J db/db 
      mice and control heterozygous (db/-) mice. The frequency of MDSCs and CD4+ T 
      cells was analyzed using flow cytometry. Serum levels of the cytokines 
      interleukin (IL)-4, IL-10, tumor necrosis factor (TNF)-alpha, and interferon (IFN)-gamma 
      were quantified using ELISA kits. Cell proliferation was assessed using 
      carboxyfluorescein succinimidyl ester (CFSE) labeling. In addition, the severity 
      of insulitis was assessed using H&E staining of the pancreata. The data showed an 
      increased frequency of CD11b+/CD33+ MDSCs and CD4+ T cells in the peripheral 
      blood of T2DM patients. In addition, there were decreased IL-4 level and 
      increased TNF-alpha and IFN-gamma levels in the serum from T2DM patients. In db/db mice, 
      an increased frequency of CD11b+/Gr-1+ MDSCs and CD4+ T cells was found in 
      splenocytes, as well as in the peripheral blood. MDSCs inhibited the 
      proliferation and modulated the cytokine secretion of CD4+ T cells in vitro and 
      delayed the development of diabetes in NOD/SCID mice. In conclusion, MDSCs 
      suppress CD4+ T cell activity and prevent the development of T2DM.
FAU - Wang, Tao
AU  - Wang T
AD  - Department of Anesthesiology, The First Affiliated Hospital, Zhengzhou 
      University, Zhengzhou 450052, China.
FAU - Wen, Yuanyuan
AU  - Wen Y
AD  - Department of Anesthesiology, The First Affiliated Hospital, Zhengzhou 
      University, Zhengzhou 450052, China.
FAU - Fan, Xiaochong
AU  - Fan X
AD  - Department of Anesthesiology, The First Affiliated Hospital, Zhengzhou 
      University, Zhengzhou 450052, China.
LA  - eng
PT  - Journal Article
PL  - China
TA  - Acta Biochim Biophys Sin (Shanghai)
JT  - Acta biochimica et biophysica Sinica
JID - 101206716
RN  - 0 (5-(6)-carboxyfluorescein diacetate succinimidyl ester)
RN  - 0 (Cytokines)
RN  - 0 (Fluoresceins)
RN  - 0 (Interleukin-1)
RN  - 0 (Succinimides)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 207137-56-2 (Interleukin-4)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Adult
MH  - Animals
MH  - CD4-Positive T-Lymphocytes/*cytology
MH  - Cell Proliferation
MH  - Cytokines/blood
MH  - Diabetes Mellitus, Type 2/blood/*immunology
MH  - Disease Progression
MH  - Female
MH  - Flow Cytometry
MH  - Fluoresceins
MH  - Heterozygote
MH  - Humans
MH  - Interferon-gamma/blood
MH  - Interleukin-1/blood
MH  - Interleukin-4/blood
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Inbred NOD
MH  - Mice, SCID
MH  - Mice, Transgenic
MH  - Middle Aged
MH  - Myeloid-Derived Suppressor Cells/*cytology
MH  - Succinimides
MH  - Tumor Necrosis Factor-alpha/blood
EDAT- 2018/03/08 06:00
MHDA- 2018/10/30 06:00
CRDT- 2018/03/08 06:00
PHST- 2017/09/15 00:00 [received]
PHST- 2018/01/29 00:00 [accepted]
PHST- 2018/03/08 06:00 [pubmed]
PHST- 2018/10/30 06:00 [medline]
PHST- 2018/03/08 06:00 [entrez]
AID - 4915834 [pii]
AID - 10.1093/abbs/gmy014 [doi]
PST - ppublish
SO  - Acta Biochim Biophys Sin (Shanghai). 2018 Apr 1;50(4):362-369. doi: 
      10.1093/abbs/gmy014.