PMID- 29516987
OWN - NLM
STAT- MEDLINE
DCOM- 20180830
LR  - 20220316
IS  - 1998-4138 (Electronic)
IS  - 1998-4138 (Linking)
VI  - 14
IP  - 1
DP  - 2018 Jan
TI  - Effects of the Akt inhibitor Src-homology 5 on proliferation and apoptosis of the 
      laryngeal squamous cell carcinoma.
PG  - 208-212
LID - 10.4103/0973-1482.199454 [doi]
AB  - OBJECTIVE: The aim of this study was to investigate the effect of the Akt 
      inhibitor Src-homology 5 (SH-5) on the proliferation and apoptosis of laryngeal 
      squamous cell carcinoma cells (LSCC; Hep-2 cells) and to elucidate the possible 
      mechanisms of such effects. MATERIALS AND METHODS: Hep-2 cells were treated with 
      different concentrations of the Akt inhibitor SH-5. The inhibitory effect of SH-5 
      on cell proliferation was examined by 
      3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, whereas 
      apoptosis was detected by flow cytometric based on Annexin V/propidium iodide 
      (PI) staining. In addition, the expression level of Akt protein was evaluated by 
      Western blot analysis. RESULTS: MTT assay results revealed that SH-5 inhibited 
      the proliferation of Hep-2 cells, with its greatest effect being observed at 2 
      muM. Apoptosis of Hep-2 cells increased following treatment with SH-5. Treatment 
      of Hep-2 cells with SH-5 decreased the expression of Akt, and this effect was 
      statistically significantly when compared with that in controls (P < 0.05). 
      CONCLUSION: SH-5 inhibited proliferation and induced apoptosis in the LSCC cell 
      line Hep-2. These effects may be caused by inhibition of the phosphoinositide 
      3-kinase-Akt signaling pathway. We believe that our data will provide useful 
      insights into LSCC target treatment and future researchn.
FAU - Jia, Tao
AU  - Jia T
AD  - Department of Otolaryngology, The Second Hospital of Shandong University, Jinan 
      250000, Shandong Province, China.
FAU - Cao, Qing-Qing
AU  - Cao QQ
AD  - Department of Health, The Second Hospital of Shandong University, Jinan 250000, 
      Shandong Province, China.
FAU - Chen, Xue-Mei
AU  - Chen XM
AD  - Department of Otolaryngology, The Second Hospital of Shandong University, Jinan 
      250000, Shandong Province, China.
FAU - Xu, Feng-Lei
AU  - Xu FL
AD  - Department of Otolaryngology, The Second Hospital of Shandong University, Jinan 
      250000, Shandong Province, China.
LA  - eng
PT  - Journal Article
PL  - India
TA  - J Cancer Res Ther
JT  - Journal of cancer research and therapeutics
JID - 101249598
RN  - 0 (1-((1-O-octadecyl-2-O-methylglycero)phospho)-3-deoxy-myo-inositol)
RN  - 0 (Inositol Phosphates)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
SB  - IM
MH  - Apoptosis/*drug effects
MH  - Carcinoma, Squamous Cell/*metabolism
MH  - Cell Line, Tumor
MH  - Cell Proliferation/drug effects
MH  - Humans
MH  - Inositol Phosphates/*pharmacology
MH  - Laryngeal Neoplasms/*metabolism
MH  - Proto-Oncogene Proteins c-akt/*antagonists & inhibitors
OTO - NOTNLM
OT  - Akt inhibitor Src-homology 5
OT  - Hep-2 cell line
OT  - apoptosis
OT  - laryngeal squamous cell carcinoma
OT  - proliferation
COIS- There are no conflicts of interest.
EDAT- 2018/03/09 06:00
MHDA- 2018/08/31 06:00
CRDT- 2018/03/09 06:00
PHST- 2018/03/09 06:00 [entrez]
PHST- 2018/03/09 06:00 [pubmed]
PHST- 2018/08/31 06:00 [medline]
AID - JCanResTher_2018_14_1_208_199454 [pii]
AID - 10.4103/0973-1482.199454 [doi]
PST - ppublish
SO  - J Cancer Res Ther. 2018 Jan;14(1):208-212. doi: 10.4103/0973-1482.199454.