PMID- 29517104
OWN - NLM
STAT- MEDLINE
DCOM- 20180829
LR  - 20220622
IS  - 1791-2431 (Electronic)
IS  - 1021-335X (Linking)
VI  - 39
IP  - 5
DP  - 2018 May
TI  - MicroRNA‑448 inhibits the progression of retinoblastoma by directly targeting 
      ROCK1 and regulating PI3K/AKT signalling pathway.
PG  - 2402-2412
LID - 10.3892/or.2018.6302 [doi]
AB  - Retinoblastoma (RB) is the most common primary intraocular malignancy during 
      infancy and childhood worldwide. Numerous microRNAs (miRNAs) contribute to RB 
      initiation and progression through the regulation of cell proliferation, cycle, 
      apoptosis, migration, invasion and metastasis. Therefore, further investigation 
      concerning the expression, roles and associated mechanisms of RB‑related miRNAs 
      may be beneficial to develop novel strategies for patients with this malignancy. 
      Recently, miRNA‑448 (miR‑448) has been reported to be aberrantly expressed and to 
      play an important role in several types of human cancer. However, the expression 
      patterns and biological roles of miR‑448 in RB have not been studied. The aim of 
      the present study was to detect the expression levels of miR‑448 and investigate 
      its functions in RB and its associated molecular mechanisms. In the present 
      study, miR‑448 was significantly downregulated in RB tissues and cell lines. 
      Upregulation of miR‑448 decreased cell proliferation and invasion and increased 
      apoptosis in RB cells. Additionally, rho‑associated coiled‑coil containing protein 
      kinase 1 (ROCK1) was validated as a novel direct target gene of miR‑448 in RB. 
      ROCK1 was overexpressed in RB tissues and inversely correlated with miR‑448 
      expression. Furthermore, ROCK1 silencing induced effects on the proliferation, 
      invasion and apoptosis of RB cells similar to those observed following miR‑448 
      overexpression. Moreover, restoration of miR‑448 expression markedly reversed the 
      effects of miR‑448 overexpression on RB cells, further supporting the hypothesis 
      that ROCK1 is a direct functional target of miR‑448 in RB. Importantly, miR‑448 
      targeted ROCK1 to inhibit the activation of the PI3K/AKT signalling pathway in 
      RB. These results demonstrated that miR‑448 may serve as a tumour suppressor in 
      RB by directly targeting ROCK1 and regulating the PI3K/AKT signalling pathway, 
      thereby suggesting that miR‑448 may be an effective therapeutic target for 
      treating this aggressive cancer.
FAU - Wu, Shen
AU  - Wu S
AD  - Beijing Institute of Ophthalmology, Beijing Tongren Eye Center, Beijing Tongren 
      Hospital, Capital Medical University, Beijing Ophthalmology and Visual Sciences 
      Key Laboratory, Beijing 100730, P.R. China.
FAU - Ai, Nanping
AU  - Ai N
AD  - Department of Ophthalmology, Chinese PLA General Hospital, Beijing 100853, P.R. 
      China.
FAU - Liu, Qian
AU  - Liu Q
AD  - Beijing Institute of Ophthalmology, Beijing Tongren Eye Center, Beijing Tongren 
      Hospital, Capital Medical University, Beijing Ophthalmology and Visual Sciences 
      Key Laboratory, Beijing 100730, P.R. China.
FAU - Zhang, Jingxue
AU  - Zhang J
AD  - Beijing Institute of Ophthalmology, Beijing Tongren Eye Center, Beijing Tongren 
      Hospital, Capital Medical University, Beijing Ophthalmology and Visual Sciences 
      Key Laboratory, Beijing 100730, P.R. China.
LA  - eng
PT  - Journal Article
PT  - Retracted Publication
DEP - 20180308
PL  - Greece
TA  - Oncol Rep
JT  - Oncology reports
JID - 9422756
RN  - 0 (3' Untranslated Regions)
RN  - 0 (MIRN448 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - EC 2.7.1.- (Phosphatidylinositol 3-Kinases)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
RN  - EC 2.7.11.1 (ROCK1 protein, human)
RN  - EC 2.7.11.1 (rho-Associated Kinases)
SB  - IM
RIN - Oncol Rep. 2022 Aug;48(2):. PMID: 35730602
MH  - 3' Untranslated Regions
MH  - Cell Line, Tumor
MH  - Cell Movement
MH  - Cell Proliferation
MH  - Disease Progression
MH  - Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - MicroRNAs/*genetics
MH  - Phosphatidylinositol 3-Kinases/metabolism
MH  - Proto-Oncogene Proteins c-akt/metabolism
MH  - Retinal Neoplasms/*genetics/metabolism/pathology
MH  - Retinoblastoma/*genetics/metabolism/pathology
MH  - Signal Transduction
MH  - rho-Associated Kinases/*genetics
EDAT- 2018/03/09 06:00
MHDA- 2018/08/30 06:00
CRDT- 2018/03/09 06:00
PHST- 2017/10/13 00:00 [received]
PHST- 2018/01/12 00:00 [accepted]
PHST- 2018/03/09 06:00 [pubmed]
PHST- 2018/08/30 06:00 [medline]
PHST- 2018/03/09 06:00 [entrez]
AID - 10.3892/or.2018.6302 [doi]
PST - ppublish
SO  - Oncol Rep. 2018 May;39(5):2402-2412. doi: 10.3892/or.2018.6302. Epub 2018 Mar 8.