PMID- 29517190
OWN - NLM
STAT- MEDLINE
DCOM- 20180601
LR  - 20180601
IS  - 0035-7715 (Print)
IS  - 0035-7715 (Linking)
VI  - 69
IP  - 1
DP  - 2018
TI  - Effect of caloric restriction on liver function in young and old ApoE/LDLr-/- 
      mice.
PG  - 37-43
AB  - BACKGROUND: Caloric restriction (CR) leads to decrease metabolic intensity, which 
      results in a reduction of oxygen consumption and the amount of free radicals. 
      This can affect the function of the liver. Studies show that caloric restriction 
      does not alter or significantly increase the enzyme activity associated with 
      gluconeogenesis, but the effect was different according to the age of the model 
      animals. OBJECTIVE: The aim of the study was to determine the effect of caloric 
      restriction on liver function in young and old ApoE/ LDLr-/- mice. MATERIAL AND 
      METHODS: Dietary experiments were performed on 2 and 5 month old male 
      ApoE/LDLr-/- mice. Animals were divided into 3 experimental groups (n=6) and fed 
      AIN'93G diet for 8 and 5 weeks, respectively. Control animals were fed ad libitum 
      (AL) and housed in a colony cages. These animals were checked for dietary intake. 
      The second group were also fed ad libitum but the animals were kept individually 
      in cages (stress AL- sAL). Similarly to sAL group, the animals from the CR group 
      were kept individually but received a 30% less diet compared to AL group. At the 
      end of the experiment animals were euthanized and the blood, liver and adipose 
      tissue have been collected. Alanine aminotransferase (ALT) as well as aspartate 
      aminotransferase (AST) were measured in plasma. Fatty acid profile was evaluated 
      (relative %) in adipose tissue (GC-MS). Liver's stetosis was assessed. Results 
      were analyzed statistically (ANOVA, STATISTICA v.10.0). RESULTS: CR ApoE/LDLr-/- 
      mice showed significantly lower body weight compared to animals, both AL and sAL. 
      There were no significant differences between ALT and AST in both younger and 
      older animals. However, negative tendencies were more pronounced in younger 
      animals. In young animals CR significantly increased liver weight compared to AL 
      (4.14 vs 3.73g/100g). In adipose tissue fatty acid profile differed in CR mice 
      compared to control in young animals. CONCLUSIONS: Caloric restriction did not 
      affect liver enzymes in mice. Caloric restriction showed similar but not 
      identical metabolic activity in young and old mice.
FAU - Kostogrys, Renata B
AU  - Kostogrys RB
AD  - Department of Clinical Biochemistry, Faculty of Medicine, Jagiellonian University 
      Collegium Medicum, Kopernika 15, 31-501 Krakow, Poland
FAU - Franczyk-Zarow, Magdalena
AU  - Franczyk-Zarow M
AD  - Department of Human Nutrition, Faculty of Food Technology, University of 
      Agriculture H. Kollataja in Cracow, Balicka 122, 30-149 Krakow, Poland
FAU - Manterys, Angelika
AU  - Manterys A
AD  - Department of Human Nutrition, Faculty of Food Technology, University of 
      Agriculture H. Kollataja in Cracow, Balicka 122, 30-149 Krakow, Poland
FAU - Wybranska, Iwona
AU  - Wybranska I
AD  - Department of Clinical Biochemistry, Faculty of Medicine, Jagiellonian University 
      Collegium Medicum, Kopernika 15, 31-501 Krakow, Poland
LA  - eng
PT  - Journal Article
PL  - Poland
TA  - Rocz Panstw Zakl Hig
JT  - Roczniki Panstwowego Zakladu Higieny
JID - 0414756
RN  - EC 2.6.1.2 (Alanine Transaminase)
RN  - EC 3.1.3.1 (Alkaline Phosphatase)
SB  - IM
MH  - Alanine Transaminase/*metabolism
MH  - Alkaline Phosphatase/*metabolism
MH  - Animals
MH  - Body Weight
MH  - *Caloric Restriction
MH  - Liver/*metabolism
MH  - Liver Diseases/metabolism
MH  - Mice
MH  - Mice, Knockout
MH  - Organ Size
OTO - NOTNLM
OT  - *caloric restriction
OT  - *liver
OT  - *ApoE/LDLr-/- mice
EDAT- 2018/03/09 06:00
MHDA- 2018/06/02 06:00
CRDT- 2018/03/09 06:00
PHST- 2018/03/09 06:00 [entrez]
PHST- 2018/03/09 06:00 [pubmed]
PHST- 2018/06/02 06:00 [medline]
PST - ppublish
SO  - Rocz Panstw Zakl Hig. 2018;69(1):37-43.