PMID- 29517846
OWN - NLM
STAT- Publisher
LR  - 20240227
IS  - 1364-3703 (Electronic)
IS  - 1464-6722 (Print)
IS  - 1364-3703 (Linking)
VI  - 19
IP  - 8
DP  - 2018 Mar 8
TI  - A dual role for proline iminopeptidase in the regulation of bacterial motility 
      and host immunity.
PG  - 2011-24
LID - 10.1111/mpp.12677 [doi]
AB  - During plant-pathogen interactions, pathogenic bacteria have evolved multiple 
      strategies to cope with the sophisticated defence systems of host plants. Proline 
      iminopeptidase (PIP) is essential to Xanthomonas campestris pv. campestris (Xcc) 
      virulence, and is conserved in many plant-associated bacteria, but its pathogenic 
      mechanism remains unclear. In this study, we found that disruption of pip in Xcc 
      enhanced its flagella-mediated bacterial motility by decreasing intracellular 
      bis-(3',5')-cyclic dimeric guanosine monophosphate (c-di-GMP) levels, whereas 
      overexpression of pip in Xcc restricted its bacterial motility by elevating 
      c-di-GMP levels. We also found that PIP is a type III secretion system-dependent 
      effector capable of eliciting a hypersensitive response in non-host, but not host 
      plants. When we transformed pip into the host plant Arabidopsis, higher bacterial 
      titres were observed in pip-overexpressing plants relative to wild-type plants 
      after Xcc inoculation. The repressive function of PIP on plant immunity was 
      dependent on PIP's enzymatic activity and acted through interference with the 
      salicylic acid (SA) biosynthetic and regulatory genes. Thus, PIP simultaneously 
      regulates two distinct regulatory networks during plant-microbe interactions, 
      i.e. it affects intracellular c-di-GMP levels to coordinate bacterial behaviour, 
      such as motility, and functions as a type III effector translocated into plant 
      cells to suppress plant immunity. Both processes provide bacteria with the 
      regulatory potential to rapidly adapt to complex environments, to utilize limited 
      resources for growth and survival in a cost-efficient manner and to improve the 
      chances of bacterial survival by helping pathogens to inhabit the internal 
      tissues of host plants.
CI  - (c) 2018 BSPP AND JOHN WILEY & SONS LTD.
FAU - Kan, Jinhong
AU  - Kan J
AD  - State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese 
      Academy of Sciences, Beijing 100101, China.
AD  - National Plant Gene Research Center, Beijing 100101, China.
AD  - College of Life Sciences, University of the Chinese Academy of Sciences, Beijing 
      100049, China.
FAU - An, Lin
AU  - An L
AD  - State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese 
      Academy of Sciences, Beijing 100101, China.
AD  - National Plant Gene Research Center, Beijing 100101, China.
AD  - College of Life Sciences, University of the Chinese Academy of Sciences, Beijing 
      100049, China.
FAU - Wu, Yao
AU  - Wu Y
AD  - State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese 
      Academy of Sciences, Beijing 100101, China.
AD  - National Plant Gene Research Center, Beijing 100101, China.
FAU - Long, Jia
AU  - Long J
AD  - College of Life Sciences, Capital Normal University, Beijing 100048, China.
FAU - Song, Liyang
AU  - Song L
AD  - State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese 
      Academy of Sciences, Beijing 100101, China.
AD  - National Plant Gene Research Center, Beijing 100101, China.
AD  - College of Life Sciences, University of the Chinese Academy of Sciences, Beijing 
      100049, China.
FAU - Fang, Rongxiang
AU  - Fang R
AD  - State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese 
      Academy of Sciences, Beijing 100101, China.
AD  - National Plant Gene Research Center, Beijing 100101, China.
FAU - Jia, Yantao
AU  - Jia Y
AUID- ORCID: 0000-0001-8700-4271
AD  - State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese 
      Academy of Sciences, Beijing 100101, China.
AD  - National Plant Gene Research Center, Beijing 100101, China.
LA  - eng
PT  - Journal Article
DEP - 20180308
PL  - England
TA  - Mol Plant Pathol
JT  - Molecular plant pathology
JID - 100954969
PMC - PMC6638124
OTO - NOTNLM
OT  - bacterial motility
OT  - pathogen-host interactions
OT  - proline iminopeptidase
OT  - repression of host immunity
EDAT- 2018/03/09 06:00
MHDA- 2018/03/09 06:00
PMCR- 2018/04/16
CRDT- 2018/03/09 06:00
PHST- 2017/09/26 00:00 [received]
PHST- 2018/03/06 00:00 [revised]
PHST- 2018/03/06 00:00 [accepted]
PHST- 2018/03/09 06:00 [pubmed]
PHST- 2018/03/09 06:00 [medline]
PHST- 2018/03/09 06:00 [entrez]
PHST- 2018/04/16 00:00 [pmc-release]
AID - MPP12677 [pii]
AID - 10.1111/mpp.12677 [doi]
PST - aheadofprint
SO  - Mol Plant Pathol. 2018 Mar 8;19(8):2011-24. doi: 10.1111/mpp.12677.