PMID- 29518395
OWN - NLM
STAT- MEDLINE
DCOM- 20180910
LR  - 20180910
IS  - 1879-0712 (Electronic)
IS  - 0014-2999 (Linking)
VI  - 826
DP  - 2018 May 5
TI  - Oridonin inhibits vascular inflammation by blocking NF-kappaB and MAPK activation.
PG  - 133-139
LID - S0014-2999(18)30128-6 [pii]
LID - 10.1016/j.ejphar.2018.02.044 [doi]
AB  - Oridonin, an active diterpenoid compound isolated from the plant Rabdosia 
      Rrubescens, has various pharmacological activities, including antioxidant, 
      anti-tumor capacities and anti-inflammation. In the present study, we explore the 
      role of oridonin in regulating endothelial inflammation and its underlying 
      mechanism. Endothelial cell-monocyte interaction was detected by 
      Leukocyte-endothelium Adhesion Assay. The protein expression was measured by 
      Western blot. NF-kappaB p65 translocation was measured by immunofluorescence. Acute 
      lung inflammation model was used to evaluate leukocyte infiltration in vivo. The 
      endothelial-leukocyte adhesion and the leukocyte transmigration were profoundly 
      reduced by oridonin. Oridonin dramatically inhibited the expression of 
      TNF-alpha-induced endothelial adhesion molecules (intercellular adhesion molecule-1 
      (ICAM-1); vascular cell adhesion molecule-1 (VCAM-1) and E-selectin) and the 
      pro-inflammatory cytokine (IL-6, IL-8 and monocyte chemoattractant 
      protein-1(MCP-1)). Oridonin suppressed the penetration of the leukocyte in the 
      acute lung injury mice model. Furthermore, Oridonin also suppressed the 
      TNF-alpha-activated MAPK and Nuclear factor kappa B (NF-kappaB) activation. Our results 
      suggest that oridonin has the anti-inflammatory properties in endothelial cells, 
      at least in part, through the suppression of MAPK and NF-kappaB activation, which may 
      have a potential therapeutic use for inflammatory vascular diseases.
CI  - Copyright (c) 2018 Elsevier B.V. All rights reserved.
FAU - Huang, Weichen
AU  - Huang W
AD  - Department of the first Orthopedics, The 2nd Affiliated Hospital, Guiyang College 
      of Traditional Chinese Medicine, Guiyang, Guizhou, China. Electronic address: 
      huangweichen1573@sina.com.
FAU - Huang, Mingcheng
AU  - Huang M
AD  - Department of Hematology, The First Affiliated Hospital, Sun Yat-sen University, 
      Guangzhou, Guangdong, China.
FAU - Ouyang, Hui
AU  - Ouyang H
AD  - Department of Gastroenterology, The 7th Affiliated Hospital, Sun Yat-Sen 
      University, ShenZhen, Guandong, China.
FAU - Peng, Jingwen
AU  - Peng J
AD  - Department of Rehabilitation Medicine, Sun Yat-sen Memorial Hospital, SunYat-sen 
      University, Guangzhou, Guandong, China.
FAU - Liang, Jiang
AU  - Liang J
AD  - Collaborative innovation center of Miao medicine, Guiyang College of Traditional 
      Chinese Medicine, Guiyang, Guizhou, China. Electronic address: 
      liangjiang1573@hotmail.com.
LA  - eng
PT  - Journal Article
DEP - 20180305
PL  - Netherlands
TA  - Eur J Pharmacol
JT  - European journal of pharmacology
JID - 1254354
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (Diterpenes, Kaurane)
RN  - 0 (NF-kappa B)
RN  - 0 (Reactive Oxygen Species)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0APJ98UCLQ (oridonin)
SB  - IM
MH  - Animals
MH  - Anti-Inflammatory Agents/*pharmacology/therapeutic use
MH  - Cell Adhesion/drug effects
MH  - Cell Communication/drug effects
MH  - Cells, Cultured
MH  - Disease Models, Animal
MH  - Diterpenes, Kaurane/*pharmacology/therapeutic use
MH  - Endothelium, Vascular/*drug effects/immunology/pathology
MH  - Humans
MH  - MAP Kinase Signaling System/*drug effects/immunology
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Monocytes/physiology
MH  - NF-kappa B/immunology/metabolism
MH  - Pneumonia/*drug therapy/immunology/pathology
MH  - Reactive Oxygen Species/metabolism
MH  - Recombinant Proteins/administration & dosage/immunology
MH  - Tumor Necrosis Factor-alpha/administration & dosage/immunology
OTO - NOTNLM
OT  - Endothelial cells
OT  - Oridonin
OT  - Vascular inflammation
EDAT- 2018/03/09 06:00
MHDA- 2018/09/11 06:00
CRDT- 2018/03/09 06:00
PHST- 2018/02/05 00:00 [received]
PHST- 2018/02/22 00:00 [revised]
PHST- 2018/02/27 00:00 [accepted]
PHST- 2018/03/09 06:00 [pubmed]
PHST- 2018/09/11 06:00 [medline]
PHST- 2018/03/09 06:00 [entrez]
AID - S0014-2999(18)30128-6 [pii]
AID - 10.1016/j.ejphar.2018.02.044 [doi]
PST - ppublish
SO  - Eur J Pharmacol. 2018 May 5;826:133-139. doi: 10.1016/j.ejphar.2018.02.044. Epub 
      2018 Mar 5.