PMID- 29519181
OWN - NLM
STAT- MEDLINE
DCOM- 20181105
LR  - 20231213
IS  - 1556-9535 (Electronic)
IS  - 1556-9527 (Linking)
VI  - 37
IP  - 3
DP  - 2018 Sep
TI  - The protective effect of beta-casomorphin-7 via promoting Foxo1 activity and 
      nuclear translocation in human lens epithelial cells.
PG  - 267-274
LID - 10.1080/15569527.2018.1445095 [doi]
AB  - PURPOSE: To investigate the protective effect of beta-casomorphin-7 (beta-CM-7) in 
      oxidative stressed human lens epithelial cells (HLECs) and to explore the 
      possible mechanism for oxidative stress in HLECs induced by high glucose. 
      METHODS: We used HLECs to determine the effect of different concentrations of 
      beta-CM-7 on cell viability by 3-(4, 5-dimethyl-2-thiazolyl)-2, 
      5-diphenyl-2-H-tetrazolimol/L bromide (MTT) assay. We used flow cytometry to 
      determine the content of reactive oxygen species (ROS) induced by oxidative 
      stress and a bioassay kit to determine the oxidant malondialdehyde (MDA) and 
      antioxidant enzyme superoxide dismutase (SOD) levels. We used Western blotting 
      and an immunofluorescence assay to determine the expression of Forkhead box o1 
      (Foxo1), SP1, and the related protein glutathione peroxidase (GSH-px) at the 
      molecular biology level as well as their intracellular localization. RESULTS: The 
      expression of Foxo1 and SP1 was weakly expressed when the glucose concentration 
      was 40 mM/L, but was highly expressed when cells were pre-treated with an 
      appropriate concentration of beta-CM-7. After pre-treatment with beta-CM-7, the cells 
      treated with 40 mM/L glucose for 48 h showed Foxo1 was transferred to the 
      nucleus, and the expression of SP1 was increased. The content of ROS and MDA in 
      the HLECs that were pre-treated with beta-CM-7 was lower than in those that was not 
      pre-treated (p <0.05). Accordingly, SOD was elevated in the cells pre-treated 
      with beta-CM-7. The relative expression of GSH-px increased with increases of Foxo1 
      and SP1. CONCLUSION: beta-CM-7 protects HLECs from oxidative damage by upregulating 
      the relative expression of Foxo1 and promoting Foxo1 nuclear translocation.
FAU - Zhu, Lihua
AU  - Zhu L
AD  - a Department of Ophthalmology , Jinzhou Medical University , Jinzhou , People's 
      Republic of China.
FAU - Li, Jia
AU  - Li J
AD  - b Department of Ophthalmology , The First Affiliated Hospital of Jinzhou Medical 
      University , Jinzhou , People's Republic of China.
FAU - Wu, Dayang
AU  - Wu D
AD  - b Department of Ophthalmology , The First Affiliated Hospital of Jinzhou Medical 
      University , Jinzhou , People's Republic of China.
FAU - Li, Bing
AU  - Li B
AD  - b Department of Ophthalmology , The First Affiliated Hospital of Jinzhou Medical 
      University , Jinzhou , People's Republic of China.
LA  - eng
PT  - Journal Article
DEP - 20180327
PL  - England
TA  - Cutan Ocul Toxicol
JT  - Cutaneous and ocular toxicology
JID - 101266892
RN  - 0 (Antioxidants)
RN  - 0 (Endorphins)
RN  - 0 (FOXO1 protein, human)
RN  - 0 (Forkhead Box Protein O1)
RN  - 0 (Peptide Fragments)
RN  - 0 (Reactive Oxygen Species)
RN  - 0 (Sp1 Transcription Factor)
RN  - 0 (SP1 protein, human)
RN  - 72122-62-4 (beta-casomorphin 7)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Antioxidants/*pharmacology/therapeutic use
MH  - Apoptosis/drug effects
MH  - Cataract/drug therapy/etiology
MH  - Cell Line
MH  - Cell Nucleus/drug effects/metabolism
MH  - Cell Survival/drug effects
MH  - Diabetes Complications/drug therapy/etiology
MH  - Endorphins/*pharmacology/therapeutic use
MH  - Epithelial Cells/cytology/*drug effects/metabolism
MH  - Forkhead Box Protein O1/*metabolism
MH  - Glucose/toxicity
MH  - Humans
MH  - Hyperglycemia/complications
MH  - Lens, Crystalline/cytology/*drug effects/metabolism
MH  - Oxidative Stress/drug effects
MH  - Peptide Fragments/*pharmacology/therapeutic use
MH  - Reactive Oxygen Species/metabolism
MH  - Sp1 Transcription Factor/metabolism
OTO - NOTNLM
OT  - Foxo1
OT  - HLECs
OT  - Sp1
OT  - beta-casomorphin-7 oxidative stress
EDAT- 2018/03/10 06:00
MHDA- 2018/11/06 06:00
CRDT- 2018/03/10 06:00
PHST- 2018/03/10 06:00 [pubmed]
PHST- 2018/11/06 06:00 [medline]
PHST- 2018/03/10 06:00 [entrez]
AID - 10.1080/15569527.2018.1445095 [doi]
PST - ppublish
SO  - Cutan Ocul Toxicol. 2018 Sep;37(3):267-274. doi: 10.1080/15569527.2018.1445095. 
      Epub 2018 Mar 27.