PMID- 29519507
OWN - NLM
STAT- MEDLINE
DCOM- 20181120
LR  - 20181120
IS  - 1873-2542 (Electronic)
IS  - 0378-1135 (Linking)
VI  - 216
DP  - 2018 Mar
TI  - Induction of porcine reproductive and respiratory syndrome virus (PRRSV)-specific 
      regulatory T lymphocytes (Treg) in the lungs and tracheobronchial lymph nodes of 
      PRRSV-infected pigs.
PG  - 13-19
LID - S0378-1135(17)31330-5 [pii]
LID - 10.1016/j.vetmic.2018.01.014 [doi]
AB  - Regulatory T lymphocytes (Treg) residing within the tissues, are known to possess 
      immunosuppressive properties which contribute to immunomodulation within the 
      organs. PRRSV infection usually weakens lung defense mechanisms, leading to 
      porcine respiratory disease complex (PRDC). Induction of circulatory Treg is one 
      of the reported mechanisms involved in PRRSV-induced immunomodulation. However, 
      whether PRRSV can induce tissue-infiltrating Treg in the lungs and lymph nodes is 
      still unclear. To investigate the effect of PRRSV on induction of porcine Treg in 
      the tissues, we isolated mononuclear cells from the lungs and tracheobronchial 
      lymph nodes, and identified the existence of Treg by flow cytometry. The results 
      demonstrated that PRRSV could induce Treg proliferation in the cultured 
      mononuclear cells derived from lungs and tracheobronchial lymph nodes, regardless 
      of the pig's PRRSV infective status. Furthermore, PRRSV-infected pigs exhibited 
      higher numbers of total tissue-infiltrating Treg and PRRSV-specific Treg in the 
      lungs and tracheobronchial lymph nodes than the PRRSV-negative pigs. To determine 
      if the lung Treg could produce an inhibitory cytokine, the numbers of 
      IL-10-producing Treg were determined. Significantly higher numbers of 
      IL-10-producing Treg in the lungs of PRRSV-infected pigs were observed. 
      Altogether, our findings indicate the potent effect of PRRSV on induction of Treg 
      in the lungs and tracheobronchial lymph nodes of the infected pigs. The findings 
      expand our understanding in PRRSV immunopathogenesis within the target organs.
CI  - Copyright (c) 2018 Elsevier B.V. All rights reserved.
FAU - Nedumpun, Teerawut
AU  - Nedumpun T
AD  - Interdisciplinary Program in Medical Microbiology, Graduate School, Chulalongkorn 
      University, Pathumwan, Bangkok 10330, Thailand.
FAU - Sirisereewan, Chaitawat
AU  - Sirisereewan C
AD  - Graduate Program in Veterinary Pathobiology, Faculty of Veterinary Science, 
      Chulalongkorn University, Pathumwan, Bangkok 10330, Thailand.
FAU - Thanmuan, Chanoknun
AU  - Thanmuan C
AD  - Faculty of Veterinary Science, Chulalongkorn University, Pathumwan, Bangkok 
      10330, Thailand.
FAU - Techapongtada, Pong
AU  - Techapongtada P
AD  - Faculty of Veterinary Science, Chulalongkorn University, Pathumwan, Bangkok 
      10330, Thailand.
FAU - Puntarotairung, Rinlapas
AU  - Puntarotairung R
AD  - Faculty of Veterinary Science, Chulalongkorn University, Pathumwan, Bangkok 
      10330, Thailand.
FAU - Naraprasertkul, Sarun
AU  - Naraprasertkul S
AD  - Faculty of Veterinary Science, Chulalongkorn University, Pathumwan, Bangkok 
      10330, Thailand.
FAU - Thanawongnuwech, Roongroje
AU  - Thanawongnuwech R
AD  - Department of Pathology, Faculty of Veterinary Science, Chulalongkorn University, 
      Pathumwan, Bangkok 10330, Thailand; Center of Excellence in Emerging Infectious 
      Diseases in Animals, Chulalongkorn University (CU-EIDAs), Pathumwan, Bangkok 
      10330, Thailand.
FAU - Suradhat, Sanipa
AU  - Suradhat S
AD  - Department of Veterinary Microbiology, Faculty of Veterinary Science, 
      Chulalongkorn University, Pathumwan, Bangkok 10330, Thailand; Center of 
      Excellence in Emerging Infectious Diseases in Animals, Chulalongkorn University 
      (CU-EIDAs), Pathumwan, Bangkok 10330, Thailand. Electronic address: 
      Sanipa.S@chula.ac.th.
LA  - eng
PT  - Journal Article
DEP - 20180203
PL  - Netherlands
TA  - Vet Microbiol
JT  - Veterinary microbiology
JID - 7705469
RN  - 0 (Cytokines)
RN  - 130068-27-8 (Interleukin-10)
SB  - IM
MH  - Animals
MH  - Cytokines
MH  - Interleukin-10/biosynthesis/immunology
MH  - Lung/*immunology/virology
MH  - Lymph Nodes/*immunology/virology
MH  - Lymphocyte Activation
MH  - Porcine Reproductive and Respiratory Syndrome/*immunology
MH  - Porcine respiratory and reproductive syndrome virus/*immunology
MH  - Swine
MH  - T-Lymphocytes, Regulatory/*immunology
OTO - NOTNLM
OT  - IL-10
OT  - Lung
OT  - PRRSV
OT  - Pig
OT  - Tracheobronchial lymph node
OT  - Treg
EDAT- 2018/03/10 06:00
MHDA- 2018/11/21 06:00
CRDT- 2018/03/10 06:00
PHST- 2017/11/14 00:00 [received]
PHST- 2018/01/22 00:00 [revised]
PHST- 2018/01/22 00:00 [accepted]
PHST- 2018/03/10 06:00 [entrez]
PHST- 2018/03/10 06:00 [pubmed]
PHST- 2018/11/21 06:00 [medline]
AID - S0378-1135(17)31330-5 [pii]
AID - 10.1016/j.vetmic.2018.01.014 [doi]
PST - ppublish
SO  - Vet Microbiol. 2018 Mar;216:13-19. doi: 10.1016/j.vetmic.2018.01.014. Epub 2018 
      Feb 3.