PMID- 29522987
OWN - NLM
STAT- MEDLINE
DCOM- 20190823
LR  - 20220408
IS  - 1878-5905 (Electronic)
IS  - 0142-9612 (Linking)
VI  - 165
DP  - 2018 May
TI  - Harnessing biochemical and structural cues for tenogenic differentiation of 
      adipose derived stem cells (ADSCs) and development of an in vitro tissue 
      interface mimicking tendon-bone insertion graft.
PG  - 79-93
LID - S0142-9612(18)30148-0 [pii]
LID - 10.1016/j.biomaterials.2018.02.046 [doi]
AB  - Tendon-bone interface tissue is extremely challenging to engineer because it 
      exhibits complex gradients of structure, composition, biologics, and cellular 
      phenotypes. As a step toward engineering these transitional zones, we initially 
      analyzed how different (topographical or biological) cues affect tenogenic 
      differentiation of adipose-derived stem cells (ADSCs). We immobilized 
      platelet-derived growth factor - BB (PDGF-BB) using polydopamine (PD) chemistry 
      on random and aligned nanofibers and investigated ADSC proliferation and 
      tenogenic differentiation. Immobilized PDGF greatly enhanced the proliferation 
      and tenogenic differentiation of ADSCs; however, nanofiber alignment had no 
      effect. Interestingly, the PDGF immobilized aligned nanofiber group showed a 
      synergistic effect with maximum expression of tenogenic markers for 14 days. We 
      also generated a nanofiber surface with spatially controlled presentation of 
      immobilized PDGF on an aligned architecture, mimicking native tendon tissue. A 
      gradient of immobilized PDGF was able to control the phenotypic differentiation 
      of ADSCs into tenocytes in a spatially controlled manner, as confirmed by 
      analysis of the expression of tenogenic markers and immunofluorescence staining. 
      We further explored the gradient formation strategy by generation of a 
      symmetrical gradient on the nanofiber surface for the generation of a structure 
      mimicking bone-patellar-tendon-bone with provision for gradient immobilization of 
      PDGF and controlled mineralization. Our study reveals that, together with 
      biochemical cues, favorable topographical cues are important for tenogenic 
      differentiation of ADSCs, and gradient presentation of PDGF can be used as a tool 
      for engineering stem cell-based bone-tendon interface tissues.
CI  - Copyright (c) 2018 Elsevier Ltd. All rights reserved.
FAU - Madhurakkat Perikamana, Sajeesh Kumar
AU  - Madhurakkat Perikamana SK
AD  - Department of Bioengineering, Hanyang University, 222 Wangsimni-ro, Seongdong-gu, 
      Seoul 04763, Republic of Korea; BK21 Plus Future Biopharmaceutical Human 
      Resources Training and Research Team, 222 Wangsimni-ro, Seongdong-gu, Seoul 
      04763, Republic of Korea.
FAU - Lee, Jinkyu
AU  - Lee J
AD  - Department of Bioengineering, Hanyang University, 222 Wangsimni-ro, Seongdong-gu, 
      Seoul 04763, Republic of Korea; BK21 Plus Future Biopharmaceutical Human 
      Resources Training and Research Team, 222 Wangsimni-ro, Seongdong-gu, Seoul 
      04763, Republic of Korea.
FAU - Ahmad, Taufiq
AU  - Ahmad T
AD  - Department of Bioengineering, Hanyang University, 222 Wangsimni-ro, Seongdong-gu, 
      Seoul 04763, Republic of Korea; BK21 Plus Future Biopharmaceutical Human 
      Resources Training and Research Team, 222 Wangsimni-ro, Seongdong-gu, Seoul 
      04763, Republic of Korea.
FAU - Kim, Eun Mi
AU  - Kim EM
AD  - Department of Bioengineering, Hanyang University, 222 Wangsimni-ro, Seongdong-gu, 
      Seoul 04763, Republic of Korea; BK21 Plus Future Biopharmaceutical Human 
      Resources Training and Research Team, 222 Wangsimni-ro, Seongdong-gu, Seoul 
      04763, Republic of Korea.
FAU - Byun, Hayeon
AU  - Byun H
AD  - Department of Bioengineering, Hanyang University, 222 Wangsimni-ro, Seongdong-gu, 
      Seoul 04763, Republic of Korea; BK21 Plus Future Biopharmaceutical Human 
      Resources Training and Research Team, 222 Wangsimni-ro, Seongdong-gu, Seoul 
      04763, Republic of Korea.
FAU - Lee, Sangmin
AU  - Lee S
AD  - Department of Bioengineering, Hanyang University, 222 Wangsimni-ro, Seongdong-gu, 
      Seoul 04763, Republic of Korea; BK21 Plus Future Biopharmaceutical Human 
      Resources Training and Research Team, 222 Wangsimni-ro, Seongdong-gu, Seoul 
      04763, Republic of Korea.
FAU - Shin, Heungsoo
AU  - Shin H
AD  - Department of Bioengineering, Hanyang University, 222 Wangsimni-ro, Seongdong-gu, 
      Seoul 04763, Republic of Korea; BK21 Plus Future Biopharmaceutical Human 
      Resources Training and Research Team, 222 Wangsimni-ro, Seongdong-gu, Seoul 
      04763, Republic of Korea. Electronic address: hshin@hanyang.ac.kr.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180227
PL  - Netherlands
TA  - Biomaterials
JT  - Biomaterials
JID - 8100316
RN  - 1B56C968OA (Becaplermin)
MH  - *Adipocytes/cytology
MH  - Becaplermin/metabolism
MH  - *Bone and Bones/cytology
MH  - Cell Differentiation
MH  - Cells, Cultured
MH  - Humans
MH  - Nanofibers
MH  - *Stem Cells/cytology
MH  - *Tendons/cytology
MH  - *Tissue Scaffolds/chemistry
OTO - NOTNLM
OT  - Bone-tendon interface
OT  - Gradient surfaces
OT  - Platelet-derived growth factor
OT  - Tenogenic differentiation
EDAT- 2018/03/10 06:00
MHDA- 2019/08/24 06:00
CRDT- 2018/03/10 06:00
PHST- 2017/11/28 00:00 [received]
PHST- 2018/02/11 00:00 [revised]
PHST- 2018/02/25 00:00 [accepted]
PHST- 2018/03/10 06:00 [pubmed]
PHST- 2019/08/24 06:00 [medline]
PHST- 2018/03/10 06:00 [entrez]
AID - S0142-9612(18)30148-0 [pii]
AID - 10.1016/j.biomaterials.2018.02.046 [doi]
PST - ppublish
SO  - Biomaterials. 2018 May;165:79-93. doi: 10.1016/j.biomaterials.2018.02.046. Epub 
      2018 Feb 27.