PMID- 29524020
OWN - NLM
STAT- MEDLINE
DCOM- 20190611
LR  - 20211204
IS  - 1573-6881 (Electronic)
IS  - 0145-479X (Linking)
VI  - 50
IP  - 3
DP  - 2018 Jun
TI  - Cross regulation between mTOR signaling and O-GlcNAcylation.
PG  - 213-222
LID - 10.1007/s10863-018-9747-y [doi]
AB  - The hexosamine biosynthetic pathway (HBP) integrates glucose, amino acids, fatty 
      acids and nucleotides metabolisms for uridine diphosphate N-acetylglucosamine 
      (UDP-GlcNAc) synthesis. UDP-GlcNAc is the nucleotide sugar donor for O-linked 
      beta-N-acetylglucosaminylation (O-GlcNAcylation) processes. O-GlcNAc transferase 
      (OGT) is the enzyme which transfers the N-acetylglucosamine (O-GlcNAc) residue 
      onto target proteins. Several studies previously showed that glucose metabolism 
      dysregulations associated with obesity, diabetes or cancer correlated with an 
      increase of OGT expression and global O-GlcNAcylation levels. Moreover, these 
      diseases present an increased activation of the nutrient sensing mammalian target 
      of rapamycin (mTOR) pathway. Other works demonstrate that mTOR regulates protein 
      O-GlcNAcylation in cancer cells through stabilization of OGT. In this context, we 
      studied the cross-talk between these two metabolic sensors in vivo in obese mice 
      predisposed to diabetes and in vitro in normal and colon cancer cells. We report 
      that levels of OGT and O-GlcNAcylation are increased in obese mice colon tissues 
      and colon cancer cells and are associated with a higher activation of mTOR 
      signaling. In parallel, treatments with mTOR regulators modulate OGT and 
      O-GlcNAcylation levels in both normal and colon cancer cells. However, 
      deregulation of O-GlcNAcylation affects mTOR signaling activation only in cancer 
      cells. Thus, a crosstalk exists between O-GlcNAcylation and mTOR signaling in 
      contexts of metabolism dysregulation associated to obesity or cancer.
FAU - Very, Ninon
AU  - Very N
AD  - CNRS, UMR 8576 - UGSF - Unite de Glycobiologie Structurale et Fonctionnelle, 
      Universite de Lille, F 59000, Lille, France.
FAU - Steenackers, Agata
AU  - Steenackers A
AD  - Laboratory of Cell Biochemistry and Biology, NIDDK, National Institutes of 
      Health, Bethesda, MD, USA.
FAU - Dubuquoy, Caroline
AU  - Dubuquoy C
AD  - INSERM, U995, LIRIC - Lille Inflammation Research International Center, CHU 
      Lille, Universite de Lille, F 59000, Lille, France.
FAU - Vermuse, Jeanne
AU  - Vermuse J
AD  - CNRS, UMR 8576 - UGSF - Unite de Glycobiologie Structurale et Fonctionnelle, 
      Universite de Lille, F 59000, Lille, France.
FAU - Dubuquoy, Laurent
AU  - Dubuquoy L
AD  - INSERM, U995, LIRIC - Lille Inflammation Research International Center, CHU 
      Lille, Universite de Lille, F 59000, Lille, France.
FAU - Lefebvre, Tony
AU  - Lefebvre T
AD  - CNRS, UMR 8576 - UGSF - Unite de Glycobiologie Structurale et Fonctionnelle, 
      Universite de Lille, F 59000, Lille, France.
FAU - El Yazidi-Belkoura, Ikram
AU  - El Yazidi-Belkoura I
AD  - CNRS, UMR 8576 - UGSF - Unite de Glycobiologie Structurale et Fonctionnelle, 
      Universite de Lille, F 59000, Lille, France. ikram.el-yazidi@univ-lille1.fr.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180309
PL  - United States
TA  - J Bioenerg Biomembr
JT  - Journal of bioenergetics and biomembranes
JID - 7701859
RN  - EC 2.4.1.- (N-Acetylglucosaminyltransferases)
RN  - EC 2.4.1.- (O-GlcNAc transferase)
RN  - EC 2.7.1.1 (mTOR protein, mouse)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
RN  - V956696549 (Acetylglucosamine)
SB  - IM
MH  - Acetylglucosamine/*metabolism
MH  - Animals
MH  - Colonic Neoplasms/metabolism
MH  - Glycosylation
MH  - Mice
MH  - Mice, Obese
MH  - N-Acetylglucosaminyltransferases/metabolism
MH  - Obesity/metabolism
MH  - Receptor Cross-Talk
MH  - *Signal Transduction
MH  - TOR Serine-Threonine Kinases/*metabolism
OTO - NOTNLM
OT  - Cancer
OT  - Colon
OT  - Metabolism
OT  - O-GlcNAcylation
OT  - Obesity
OT  - mTOR signaling
EDAT- 2018/03/11 06:00
MHDA- 2019/06/14 06:00
CRDT- 2018/03/11 06:00
PHST- 2017/10/19 00:00 [received]
PHST- 2018/02/15 00:00 [accepted]
PHST- 2018/03/11 06:00 [pubmed]
PHST- 2019/06/14 06:00 [medline]
PHST- 2018/03/11 06:00 [entrez]
AID - 10.1007/s10863-018-9747-y [pii]
AID - 10.1007/s10863-018-9747-y [doi]
PST - ppublish
SO  - J Bioenerg Biomembr. 2018 Jun;50(3):213-222. doi: 10.1007/s10863-018-9747-y. Epub 
      2018 Mar 9.