PMID- 29525100
OWN - NLM
STAT- MEDLINE
DCOM- 20180827
LR  - 20181202
IS  - 1873-0191 (Electronic)
IS  - 0928-4931 (Linking)
VI  - 86
DP  - 2018 May 1
TI  - Preparation and characterization of gelatin-chitosan-nanobeta-TCP based scaffold for 
      orthopaedic application.
PG  - 83-94
LID - S0928-4931(17)30384-3 [pii]
LID - 10.1016/j.msec.2018.02.001 [doi]
AB  - The primary aim of this study was to fabricate gelatin/chitosan/beta-TCP (GCT) 
      composite scaffold to improve its compressive mechanical behaviour and in-vivo 
      biocompatibility with predictable degradation rate. Beta tricalcium phosphate 
      (beta-TCP) powder was synthesized in size range between 70-100 nm using aqueous 
      precipitation route at a fixed Ca/P molar ratio of 1.5:1 at pH 10 and after 
      subsequent heat treatment of as precipitated powder at 800  degrees C for 4 hours. The 
      composite scaffolds were fabricated using solid-liquid phase separation of the 
      slurry containing gelatin, chitosan, beta-tricalcium phosphate in varying proportion 
      and subsequent lyophilisation of the phase separated mixture. The prepared 
      scaffolds exhibited high porosity (>80%) with pore sizes ranging between 
      78-382 mum as determined using Hg-porosimetry. SEM result revealed that 
      incorporation of beta-TCP to the extent of 30 wt% resulted in well-shaped and 
      uniformly distributed interconnected pores of average pore size of 120 +/- 18.6 mum 
      in it. Compressive strength of the scaffolds was increased from 0.8 MPa to 
      2.45 MPa on increase in beta-TCP content from 10 wt%-30 wt% in the prepared 
      scaffold. Human Umbilical Cord derived mesenchymal stem cells (MSCs) exhibited 
      higher degree of lamellopodia and fillopodia extensions and better spreading 
      behaviour onto GCT30 scaffold. MTT assay and immunocytochemistry studies with 
      cultured MSCs revealed that GCT30 scaffolds were more conducive to MSC's 
      proliferation and differentiation into osteoblast lineage. In vivo implantation 
      of GCT30 scaffold subcutaneously into mice did not indicate any significant 
      inflammatory reaction, but ongoing vascularization.
CI  - Copyright (c) 2018. Published by Elsevier B.V.
FAU - Maji, Kanchan
AU  - Maji K
AD  - Department of Ceramic Engineering, NIT Rourkela, Odisha, India.
FAU - Dasgupta, Sudip
AU  - Dasgupta S
AD  - Department of Ceramic Engineering, NIT Rourkela, Odisha, India. Electronic 
      address: dasguptas@nitrkl.ac.in.
FAU - Pramanik, Krishna
AU  - Pramanik K
AD  - Department of Biotechnology and Medical Engineering, NIT Rourkela, Odisha, India. 
      Electronic address: Kpr@nitrkl.ac.in.
FAU - Bissoyi, Akalabya
AU  - Bissoyi A
AD  - Department of Biotechnology and Medical Engineering, NIT Rourkela, Odisha, India.
LA  - eng
PT  - Journal Article
DEP - 20180203
PL  - Netherlands
TA  - Mater Sci Eng C Mater Biol Appl
JT  - Materials science & engineering. C, Materials for biological applications
JID - 101484109
RN  - 0 (Biocompatible Materials)
RN  - 0 (Calcium Phosphates)
RN  - 0 (beta-tricalcium phosphate)
RN  - 104982-03-8 (Osteocalcin)
RN  - 9000-70-8 (Gelatin)
RN  - 9012-76-4 (Chitosan)
SB  - IM
MH  - Animals
MH  - Biocompatible Materials/chemical synthesis/*chemistry/pharmacology
MH  - Calcium Phosphates/*chemistry
MH  - Cell Differentiation/drug effects
MH  - Cell Proliferation/drug effects
MH  - Cells, Cultured
MH  - Chitosan/*chemistry
MH  - Compressive Strength
MH  - Gelatin/*chemistry
MH  - Humans
MH  - Mesenchymal Stem Cells/cytology/metabolism
MH  - Mice
MH  - Microscopy, Fluorescence
MH  - Nanostructures/*chemistry
MH  - Osteocalcin/metabolism
MH  - Porosity
MH  - Prostheses and Implants
MH  - Tissue Engineering
MH  - Tissue Scaffolds/chemistry
MH  - Umbilical Cord/cytology
OTO - NOTNLM
OT  - Chitosan
OT  - Gelatin
OT  - Mesenchymal stem cell
OT  - Tissue engineering scaffold
OT  - beta-TCP
EDAT- 2018/03/12 06:00
MHDA- 2018/08/28 06:00
CRDT- 2018/03/12 06:00
PHST- 2017/01/31 00:00 [received]
PHST- 2017/11/20 00:00 [revised]
PHST- 2018/02/02 00:00 [accepted]
PHST- 2018/03/12 06:00 [entrez]
PHST- 2018/03/12 06:00 [pubmed]
PHST- 2018/08/28 06:00 [medline]
AID - S0928-4931(17)30384-3 [pii]
AID - 10.1016/j.msec.2018.02.001 [doi]
PST - ppublish
SO  - Mater Sci Eng C Mater Biol Appl. 2018 May 1;86:83-94. doi: 
      10.1016/j.msec.2018.02.001. Epub 2018 Feb 3.