PMID- 29525124
OWN - NLM
STAT- MEDLINE
DCOM- 20180521
LR  - 20181202
IS  - 1873-3778 (Electronic)
IS  - 0021-9673 (Linking)
VI  - 1546
DP  - 2018 Apr 20
TI  - Sensitive determination of brassinosteroids by solid phase boronate affinity 
      labeling coupled with liquid chromatography-tandem mass spectrometry.
PG  - 10-17
LID - S0021-9673(18)30256-5 [pii]
LID - 10.1016/j.chroma.2018.02.058 [doi]
AB  - Brassinosteroids (BRs) are regarded as the sixth plant hormone that is widely 
      distributed in the plant kingdom. Sensitive quantification of BRs will be greatly 
      benefit to illuminate the detail mechanisms about how BRs play crucial role in 
      plant developmental processes such as cell division, cell expansion, 
      cytodifferentiation, seed germination, vegetative growth and resisting biological 
      or abiotic stress. In the current study, we developed a method for rapid and 
      sensitive determination of endogenous BRs in plant tissues by combining LC-MS and 
      a novel sample preparation strategy, in which the plant tissue extract was 
      supplied to solid phase boronate affinity labeling and extraction, followed by 
      desorption and salt-induced phase transition extraction for further purification. 
      Under the optimized conditions, good linearity was obtained for 6 BR with 
      correlation coefficients (r) ranging from 0.9988 to 0.9999. The limits of 
      detection (LODs, S/N = 3) ranged from 1.4 to 2.8 pg mL(-1). The recoveries were 
      between 93.4% and 116.2% with the relative standard deviations (RSDs) ranging 
      from 2.8% to 15.8%. Finally, the developed method was successfully applied to the 
      analysis of 6 endogenous BR in various plant tissues including 20 mg FW Oryza 
      sativa shoot, 10 mg FW Oryza sativa root, 20 mg FW Arabidopsis thaliana shoot, 4 
      Arabidopsis thaliana flowers (2.8 mg) and one Brassica napus stamen (3.0 mg) with 
      concentration ranging from 0.26 to 157.28 ng g(-1) FW.
CI  - Copyright (c) 2018 Elsevier B.V. All rights reserved.
FAU - Luo, Xiao-Tong
AU  - Luo XT
AD  - Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of 
      Education), Department of Chemistry, Wuhan University, Wuhan 430072, China.
FAU - Cai, Bao-Dong
AU  - Cai BD
AD  - Wuhan Greensword Creation Technology Co., Ltd, Wuhan 430075, China.
FAU - Yu, Lei
AU  - Yu L
AD  - Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of 
      Education), Department of Chemistry, Wuhan University, Wuhan 430072, China.
FAU - Ding, Jun
AU  - Ding J
AD  - Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of 
      Education), Department of Chemistry, Wuhan University, Wuhan 430072, China.
FAU - Feng, Yu-Qi
AU  - Feng YQ
AD  - Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of 
      Education), Department of Chemistry, Wuhan University, Wuhan 430072, China. 
      Electronic address: yqfeng@whu.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20180228
PL  - Netherlands
TA  - J Chromatogr A
JT  - Journal of chromatography. A
JID - 9318488
RN  - 0 (Affinity Labels)
RN  - 0 (Boronic Acids)
RN  - 0 (Brassinosteroids)
RN  - 0 (Isoquinolines)
RN  - 0 (Plant Extracts)
RN  - 0 (Plant Growth Regulators)
SB  - IM
MH  - Affinity Labels/*chemistry
MH  - Boronic Acids/*chemistry
MH  - Brassica napus/chemistry
MH  - Brassinosteroids/*analysis/chemistry
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Isoquinolines/chemistry
MH  - Limit of Detection
MH  - Plant Extracts/chemistry
MH  - Plant Growth Regulators/chemistry
MH  - Reproducibility of Results
MH  - Signal Processing, Computer-Assisted
MH  - Solid Phase Extraction/*methods
MH  - Tandem Mass Spectrometry/*methods
OTO - NOTNLM
OT  - Brassinosteroids
OT  - Mass spectrometry
OT  - Salt-induced phase transition extraction
OT  - Solid phase boronate affinity labeling
EDAT- 2018/03/12 06:00
MHDA- 2018/05/22 06:00
CRDT- 2018/03/12 06:00
PHST- 2018/01/09 00:00 [received]
PHST- 2018/02/21 00:00 [revised]
PHST- 2018/02/27 00:00 [accepted]
PHST- 2018/03/12 06:00 [pubmed]
PHST- 2018/05/22 06:00 [medline]
PHST- 2018/03/12 06:00 [entrez]
AID - S0021-9673(18)30256-5 [pii]
AID - 10.1016/j.chroma.2018.02.058 [doi]
PST - ppublish
SO  - J Chromatogr A. 2018 Apr 20;1546:10-17. doi: 10.1016/j.chroma.2018.02.058. Epub 
      2018 Feb 28.