PMID- 29527912
OWN - NLM
STAT- MEDLINE
DCOM- 20181126
LR  - 20190612
IS  - 1802-9973 (Electronic)
IS  - 0862-8408 (Linking)
VI  - 67
IP  - 3
DP  - 2018 Jul 17
TI  - Brain-derived neurotrophic factor modulates intestinal barrier by inhibiting 
      intestinal epithelial cells apoptosis in mice.
PG  - 475-485
AB  - We aimed to investigate the effects of brain-derived neurotrophic factor (BDNF) 
      on apoptosis of intestinal epithelial cells (IECs) and alterations of intestinal 
      barrier integrity using BDNF knock-out mice model. Colonic tissues from BDNF(+/+) 
      mice and BDNF(+/-) mice were prepared for this study. The integrity of colonic 
      mucosa was evaluated by measuring trans-mucosa electrical resistance and tissue 
      conductance in Ussing chamber. The colonic epithelial structure was analyzed by 
      transmission electron microscopy. Apoptosis involvement was determined with TUNEL 
      staining, active caspase-3 immunostaining and Western blotting for the protein 
      expression of active caspase-3, Bax and Bcl-2. The expression levels and 
      distribution of tight junction proteins were evaluated by immunohistochemistry or 
      Western blots. Compared with BDNF(+/+) mice, BDNF(+/-) mice displayed impaired 
      integrity and ultrastructure alterations in their colonic mucosa, which was 
      characterized by diminished microvilli, mitochondrial swelling and epithelial 
      cells apoptosis. Altered intestinal barrier function was linked to excessive 
      apoptosis of IECs demonstrated by the higher proportion of TUNEL-positive 
      apoptotic cells and enhanced caspase activities in BDNF(+/-) mice. Increased 
      expression of Bax and claudin-2 proteins and reduced Bcl-2 and tight junction 
      proteins (occludin, ZO-1 and claudin-1) expression were also detected in the 
      colonic mucosa of BDNF(+/-) mice. BDNF may play a role in the maintenance of 
      intestinal barrier integrity via its anti-apoptotic properties.
FAU - Zhao, D Y
AU  - Zhao DY
AD  - Department of Gastroenterology, Puyang Oilfield General Hospital, Puyang, China; 
      Department of Gastroenterology, Qilu Hospital, Shandong University, Jinan, P.R. 
      China. yuyanbo2000@126.com.
FAU - Zhang, W X
AU  - Zhang WX
FAU - Qi, Q Q
AU  - Qi QQ
FAU - Long, X
AU  - Long X
FAU - Li, X
AU  - Li X
FAU - Yu, Y B
AU  - Yu YB
FAU - Zuo, X L
AU  - Zuo XL
LA  - eng
PT  - Journal Article
DEP - 20180312
PL  - Czech Republic
TA  - Physiol Res
JT  - Physiological research
JID - 9112413
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Tight Junction Proteins)
RN  - EC 3.4.22.- (Caspase 3)
SB  - IM
MH  - Animals
MH  - *Apoptosis
MH  - Brain-Derived Neurotrophic Factor/*physiology
MH  - Caspase 3/metabolism
MH  - Epithelial Cells/*physiology
MH  - Female
MH  - Intestinal Mucosa/*metabolism/ultrastructure
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Tight Junction Proteins/metabolism
EDAT- 2018/03/13 06:00
MHDA- 2018/11/27 06:00
CRDT- 2018/03/13 06:00
PHST- 2018/03/13 06:00 [pubmed]
PHST- 2018/11/27 06:00 [medline]
PHST- 2018/03/13 06:00 [entrez]
AID - 933641 [pii]
AID - 10.33549/physiolres.933641 [doi]
PST - ppublish
SO  - Physiol Res. 2018 Jul 17;67(3):475-485. doi: 10.33549/physiolres.933641. Epub 
      2018 Mar 12.