PMID- 29530791
OWN - NLM
STAT- MEDLINE
DCOM- 20190729
LR  - 20190729
IS  - 1873-6300 (Electronic)
IS  - 0891-0618 (Linking)
VI  - 91
DP  - 2018 Sep
TI  - Overexpression of miR-219 promotes differentiation of human induced pluripotent 
      stem cells into pre-oligodendrocyte.
PG  - 8-16
LID - S0891-0618(17)30209-0 [pii]
LID - 10.1016/j.jchemneu.2018.03.001 [doi]
AB  - Oligodendrocytes play critical roles in the central nervous system (CNS) thorough 
      producing myelin sheaths around axons. There are a variety of approaches to 
      produce oligodendrocytes in vitro and in vivo which are a subject of interest in 
      many studies. A new approach to induce this differentiation is using microRNA 219 
      (miR-219). However, this new approach suffers from a lack of studies regarding 
      the effect of miR-219 on differentiating human induced pluripotent stem cells 
      (hiPSCs) to oligodendrocytes. This study aimed to assess the impact of 
      miR-219-overexpression on hiPSCs. Initially, hiPSCs were induced with basic 
      fibroblast growth factor (bFGF), epidermalgrowth factor (EGF) and 
      platelet-derived growth factor (PDGF)-AA, then, miR-219- green fluorescent 
      protein (GFP)-expressing lentiviruses were utilized for cell infection. 
      Microscopic observation revealed significant morphological changes and data 
      obtained from quantitative reverse transcription PCR and immunofluorescence 
      analysis of differentiated cells showed that the expression of various 
      oligodendrocyte stage-specific markers such as Nestin, Olig2, Sox10, PDGFRalpha, 
      A2B5, O4, and MBP increased. In addition, higher expressions of 
      pre-oligodendrocyte markers were detected in the cells transduced with miR-219 
      lentivirus in comparison with the cells treated with triiodothyronine (T3). These 
      results suggest that overexpression of miR-219 promotes differentiation of hiPSCs 
      to pre-oligodendrocyte cells, providing a potential source for cell therapy by 
      replacing and restoring the lost cell function in neurodegenerative and 
      demyelinating diseases.
CI  - Copyright (c) 2018. Published by Elsevier B.V.
FAU - Nazari, Bahareh
AU  - Nazari B
AD  - Department of Medical Biotechnology, School of Advanced Technologies in Medicine, 
      Tehran University of Medical Sciences, Tehran, Iran.
FAU - Soleimani, Masoud
AU  - Soleimani M
AD  - Department of Hematology, Faculty of Medical Sciences, TarbiatModares University, 
      Tehran, Iran.
FAU - Ebrahimi-Barough, Somayeh
AU  - Ebrahimi-Barough S
AD  - Department of Tissue Engineering and Applied Cell Sciences, School of Advanced 
      Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.
FAU - Enderami, Seyed Ehsan
AU  - Enderami SE
AD  - Stem Cell Technology Research Center, Tehran, Iran.
FAU - Kazemi, Mansure
AU  - Kazemi M
AD  - Department of Tissue Engineering and Applied Cell Sciences, School of Advanced 
      Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.
FAU - Negahdari, Babak
AU  - Negahdari B
AD  - Department of Medical Biotechnology, School of Advanced Technologies in Medicine, 
      Tehran University of Medical Sciences, Tehran, Iran.
FAU - Sadroddiny, Esmaeil
AU  - Sadroddiny E
AD  - Department of Medical Biotechnology, School of Advanced Technologies in Medicine, 
      Tehran University of Medical Sciences, Tehran, Iran. Electronic address: 
      sadroddiny@sina.tums.ac.ir.
FAU - Ai, Jafar
AU  - Ai J
AD  - Department of Tissue Engineering and Applied Cell Sciences, School of Advanced 
      Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran. 
      Electronic address: Jafar_ai@tums.ac.ir.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180309
PL  - Netherlands
TA  - J Chem Neuroanat
JT  - Journal of chemical neuroanatomy
JID - 8902615
RN  - 0 (MIRN219 microRNA, human)
RN  - 0 (MicroRNAs)
MH  - Cell Differentiation/*genetics
MH  - Humans
MH  - Induced Pluripotent Stem Cells/*cytology/metabolism
MH  - MicroRNAs/*biosynthesis
MH  - Oligodendrocyte Precursor Cells/*cytology/metabolism
MH  - Oligodendroglia/*cytology/metabolism
OTO - NOTNLM
OT  - Differentiation
OT  - Induced pluripotent stem cells
OT  - Oligodendrocyte
OT  - miR-219
EDAT- 2018/03/14 06:00
MHDA- 2019/07/30 06:00
CRDT- 2018/03/14 06:00
PHST- 2017/10/07 00:00 [received]
PHST- 2018/03/04 00:00 [revised]
PHST- 2018/03/05 00:00 [accepted]
PHST- 2018/03/14 06:00 [pubmed]
PHST- 2019/07/30 06:00 [medline]
PHST- 2018/03/14 06:00 [entrez]
AID - S0891-0618(17)30209-0 [pii]
AID - 10.1016/j.jchemneu.2018.03.001 [doi]
PST - ppublish
SO  - J Chem Neuroanat. 2018 Sep;91:8-16. doi: 10.1016/j.jchemneu.2018.03.001. Epub 
      2018 Mar 9.