PMID- 29531586
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 1857-9655 (Print)
IS  - 1857-9655 (Electronic)
IS  - 1857-9655 (Linking)
VI  - 6
IP  - 2
DP  - 2018 Feb 15
TI  - Effect of a Histone Deacetylases Inhibitor of IL-18 and TNF-Alpha Secretion in 
      Vitro.
PG  - 269-273
LID - 10.3889/oamjms.2018.075 [doi]
AB  - BACKGROUND: Interleukin-18 (IL-18) and Tumor Necrosis Factor-alpha (TNF-alpha) are 
      proinflammatory cytokines that increased the development of Th1 immune response, 
      but have a different type of regulation of the gene expression. Whereas TNF-alpha has 
      an inducible expression, IL-18 is translated as an inactive protein and required 
      proteolytic cleavage by Casp-1 in inflammasome complexes. AIM: To investigate the 
      effect of the histone deacetylases inhibitor Suberoylanilide Hydroxamic Acid 
      (SAHA) on the gene expression and secretion of both cytokines, IL-18 and TNF-alpha, 
      according to their contribution to the cancer development and anticancer 
      immunity. METHODS: Isolated peripheral blood mononuclear cells (PBMC) were 
      stimulated with LPS and C3bgp with or without SAHA. Cytokine production was 
      assessed by ELISA at 6 and 24h. RESULTS: IL-18 and TNF-alpha secretion was 
      significantly increased at 6h and 24h in response to stimulation. TNF-alpha 
      production from stimulated PBMC was downregulated by SAHA at 6 and 24h. Treatment 
      with SAHA does not inhibit the secretion of IL-18 significantly either at 6 or 
      24h of stimulation. CONCLUSION: The inhibition of histone deacetylases by SAHA 
      does not influence the inflammasome-dependent production of immunologically 
      active IL-18. In contrast, the production of proinflammatory TNF-alpha in cultures 
      was mediated by the activity of HDAC class I and class II enzymes.
FAU - Dobreva, Zlatka Georgieva
AU  - Dobreva ZG
AD  - Department of Molecular Biology, Immunology and Medical Genetics, Medical 
      Faculty, Trakia University, Armeiska 11, Stara Zagora 6000, Bulgaria.
FAU - Grigorov, Boncho Grigorov
AU  - Grigorov BG
AD  - Department of Molecular Biology, Immunology and Medical Genetics, Medical 
      Faculty, Trakia University, Armeiska 11, Stara Zagora 6000, Bulgaria.
FAU - Stanilova, Spaska Angelova
AU  - Stanilova SA
AD  - Department of Molecular Biology, Immunology and Medical Genetics, Medical 
      Faculty, Trakia University, Armeiska 11, Stara Zagora 6000, Bulgaria.
LA  - eng
PT  - Journal Article
DEP - 20180210
PL  - North Macedonia
TA  - Open Access Maced J Med Sci
JT  - Open access Macedonian journal of medical sciences
JID - 101662294
PMC - PMC5839430
OTO - NOTNLM
OT  - HDAC
OT  - IL-18
OT  - PBMC
OT  - SAHA
OT  - TNF-alpha
EDAT- 2018/03/14 06:00
MHDA- 2018/03/14 06:01
PMCR- 2018/02/10
CRDT- 2018/03/14 06:00
PHST- 2017/11/20 00:00 [received]
PHST- 2017/12/04 00:00 [revised]
PHST- 2017/12/05 00:00 [accepted]
PHST- 2018/03/14 06:00 [entrez]
PHST- 2018/03/14 06:00 [pubmed]
PHST- 2018/03/14 06:01 [medline]
PHST- 2018/02/10 00:00 [pmc-release]
AID - OAMJMS-6-269 [pii]
AID - 10.3889/oamjms.2018.075 [doi]
PST - epublish
SO  - Open Access Maced J Med Sci. 2018 Feb 10;6(2):269-273. doi: 
      10.3889/oamjms.2018.075. eCollection 2018 Feb 15.