PMID- 29532368
OWN - NLM
STAT- MEDLINE
DCOM- 20180918
LR  - 20220330
IS  - 1559-1166 (Electronic)
IS  - 0895-8696 (Linking)
VI  - 64
IP  - 4
DP  - 2018 Apr
TI  - Valproic Acid Promotes Survival of Facial Motor Neurons in Adult Rats After 
      Facial Nerve Transection: a Pilot Study.
PG  - 512-522
LID - 10.1007/s12031-018-1051-0 [doi]
AB  - Valproic acid (VPA), a medication primarily used to treat epilepsy and bipolar 
      disorder, has been applied to the repair of central and peripheral nervous system 
      injury. The present study investigated the effect of VPA on functional recovery, 
      survival of facial motor neurons (FMNs), and expression of proteins in rats after 
      facial nerve trunk transection by functional measurement, Nissl staining, TUNEL, 
      immunofluorescence, and Western blot. Following facial nerve injury, all rats in 
      group VPA showed a better functional recovery, which was significant at the given 
      time, compared with group NS. The Nissl staining results demonstrated that the 
      number of FMNs survival in group VPA was higher than that in group normal saline 
      (NS). TUNEL staining showed that axonal injury of facial nerve could lead to 
      neuronal apoptosis of FMNs. But treatment of VPA significantly reduced cell 
      apoptosis by decreasing the expression of Bax protein and increased neuronal 
      survival by upregulating the level of brain-derived neurotrophic factor (BDNF) 
      and growth associated protein-43 (GAP-43) expression in injured FMNs compared 
      with group NS. Overall, our findings suggest that VPA may advance functional 
      recovery, reduce lesion-induced apoptosis, and promote neuron survival after 
      facial nerve transection in rats. This study provides an experimental evidence 
      for better understanding the mechanism of injury and repair of peripheral facial 
      paralysis.
FAU - Zhang, Lili
AU  - Zhang L
AD  - Department of Otolaryngology-Head and Neck Surgery, Shandong Provincial Hospital 
      Affiliated to Shandong University, Jinan, 250021, China.
AD  - Department of Otolaryngology-Head and Neck Surgery, Yantai Affiliated Hospital of 
      Binzhou Medical University, Yantai, 264199, China.
FAU - Fan, Zhaomin
AU  - Fan Z
AD  - Department of Otolaryngology-Head and Neck Surgery, Shandong Provincial Hospital 
      Affiliated to Shandong University, Jinan, 250021, China.
FAU - Han, Yuechen
AU  - Han Y
AD  - Department of Otolaryngology-Head and Neck Surgery, Shandong Provincial Hospital 
      Affiliated to Shandong University, Jinan, 250021, China.
FAU - Xu, Lei
AU  - Xu L
AD  - Department of Otolaryngology-Head and Neck Surgery, Shandong Provincial Hospital 
      Affiliated to Shandong University, Jinan, 250021, China.
FAU - Liu, Wenwen
AU  - Liu W
AD  - Shandong Provincial Key Laboratory of Otology, Jinan, 250022, China.
FAU - Bai, Xiaohui
AU  - Bai X
AD  - Shandong Provincial Key Laboratory of Otology, Jinan, 250022, China.
FAU - Zhou, Meijuan
AU  - Zhou M
AD  - Shandong Provincial Key Laboratory of Otology, Jinan, 250022, China.
FAU - Li, Jianfeng
AU  - Li J
AD  - Shandong Provincial Key Laboratory of Otology, Jinan, 250022, China.
AD  - Institute of Eye and ENT, Shandong Provincial Hospital Affiliated to Shandong 
      University, Jinan, 250022, China.
FAU - Wang, Haibo
AU  - Wang H
AUID- ORCID: 0000-0001-8196-9291
AD  - Department of Otolaryngology-Head and Neck Surgery, Shandong Provincial Hospital 
      Affiliated to Shandong University, Jinan, 250021, China. wang.hb7585@hotmail.com.
AD  - Shandong Provincial Key Laboratory of Otology, Jinan, 250022, China. 
      wang.hb7585@hotmail.com.
AD  - Institute of Eye and ENT, Shandong Provincial Hospital Affiliated to Shandong 
      University, Jinan, 250022, China. wang.hb7585@hotmail.com.
LA  - eng
GR  - 2014CB541703/the National 973 Basic Research Program of China/
GR  - 81670932/the National Natural Science Foundation of China/
PT  - Journal Article
DEP - 20180312
PL  - United States
TA  - J Mol Neurosci
JT  - Journal of molecular neuroscience : MN
JID - 9002991
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (GAP-43 Protein)
RN  - 0 (Neuroprotective Agents)
RN  - 614OI1Z5WI (Valproic Acid)
SB  - IM
MH  - Animals
MH  - Apoptosis
MH  - Brain-Derived Neurotrophic Factor/genetics/metabolism
MH  - Facial Nerve Injuries/*drug therapy
MH  - Female
MH  - GAP-43 Protein/genetics/metabolism
MH  - Male
MH  - Neurons/drug effects/metabolism
MH  - Neuroprotective Agents/*pharmacology/therapeutic use
MH  - Rats
MH  - Rats, Wistar
MH  - Valproic Acid/*pharmacology/therapeutic use
OTO - NOTNLM
OT  - Neuronal apoptosis
OT  - Neuroprotective effect
OT  - Peripheral facial paralysis
OT  - Valproic acid
EDAT- 2018/03/14 06:00
MHDA- 2018/09/19 06:00
CRDT- 2018/03/14 06:00
PHST- 2017/11/18 00:00 [received]
PHST- 2018/02/22 00:00 [accepted]
PHST- 2018/03/14 06:00 [pubmed]
PHST- 2018/09/19 06:00 [medline]
PHST- 2018/03/14 06:00 [entrez]
AID - 10.1007/s12031-018-1051-0 [pii]
AID - 10.1007/s12031-018-1051-0 [doi]
PST - ppublish
SO  - J Mol Neurosci. 2018 Apr;64(4):512-522. doi: 10.1007/s12031-018-1051-0. Epub 2018 
      Mar 12.