PMID- 29540180
OWN - NLM
STAT- MEDLINE
DCOM- 20190613
LR  - 20190613
IS  - 1479-5876 (Electronic)
IS  - 1479-5876 (Linking)
VI  - 16
IP  - 1
DP  - 2018 Mar 14
TI  - Comparison of human bone marrow stromal cells cultured in human platelet growth 
      factors and fetal bovine serum.
PG  - 65
LID - 10.1186/s12967-018-1400-3 [doi]
LID - 65
AB  - BACKGROUND: Bone marrow stromal cells (BMSCs) have classically been cultured in 
      media supplemented with fetal bovine serum (FBS). As an alternative to FBS, 
      pooled solvent detergent apheresis platelets, HPGF-C18, was evaluated for BMSC 
      culture. METHODS: A comparison of passage 2 BMSC growth revealed that 10% 
      HPGF-C18 produced similar cell numbers as 20% FBS. Marrow aspirates from 5 
      healthy subjects were cultured for 4 passages in 10% HPGF-C18 or 20% FBS and were 
      analyzed for proliferation, colony formation efficiency (CFE), surface marker 
      expression, suppression of mixed lymphocyte reactions (MLRs), global gene and 
      microRNA expression analysis. BMSC supernatant cytokine and growth factor 
      concentrations were also compared. RESULTS: Primary cultures of marrow aspirates 
      in 10% HPGF-C18 and 20% FBS yielded similar numbers and CFE. After 4 passages, 
      10% HPGF-C18 and 20% FBS yielded similar numbers of BMSCs, surface marker 
      expression patterns and immunosuppression effects. Gene and microRNA expression 
      analysis revealed that BMSCs cultured under the two conditions had distinct 
      expression profiles. Gene Set Enrichment Analysis (GSEA) revealed 
      HPGF-C18-cultured BMSCs were enriched in metabolic processing and biosynthetic 
      pathways, cell proliferation and cell cycle pathways, and immune response 
      pathways. FBS-cultured BMSCs were enriched in MAPK signaling, TGF-beta signaling, 
      cell adhesion and extracellular matrix pathways. Differently expressed microRNAs 
      were related to the osteogenesis of BMSCs. The supernatant of HPGF-C18 BMSCs had 
      higher levels of PEDF and TGFB1 and lower levels of IL6, VEGF, SDF1 and PLGF. 
      CONCLUSIONS: Traditional measures, expansion, surface marker expression and 
      inhibition of MLRs suggest that BMSC cultured in HPGF-C18 and FBS were similar, 
      but analysis at the molecular level revealed many differences. BMSCs cultured in 
      HPGF-C18 should be assessed in specific functional assays that reflect 
      application-specific potency before substituting FBS with HPGF-C18.
FAU - Ren, Jiaqiang
AU  - Ren J
AD  - Cell Processing Section, Department of Transfusion Medicine, Clinical Center, 
      National Institutes of Health, 10 Center Drive-MSC-1184, Building 10, Room 3C720, 
      Bethesda, MD, 20892-1184, USA.
FAU - Ward, Dawn
AU  - Ward D
AD  - Cell Processing Section, Department of Transfusion Medicine, Clinical Center, 
      National Institutes of Health, 10 Center Drive-MSC-1184, Building 10, Room 3C720, 
      Bethesda, MD, 20892-1184, USA.
FAU - Chen, Steven
AU  - Chen S
AD  - Cell Processing Section, Department of Transfusion Medicine, Clinical Center, 
      National Institutes of Health, 10 Center Drive-MSC-1184, Building 10, Room 3C720, 
      Bethesda, MD, 20892-1184, USA.
FAU - Tran, Katherine
AU  - Tran K
AD  - Cell Processing Section, Department of Transfusion Medicine, Clinical Center, 
      National Institutes of Health, 10 Center Drive-MSC-1184, Building 10, Room 3C720, 
      Bethesda, MD, 20892-1184, USA.
FAU - Jin, Ping
AU  - Jin P
AD  - Cell Processing Section, Department of Transfusion Medicine, Clinical Center, 
      National Institutes of Health, 10 Center Drive-MSC-1184, Building 10, Room 3C720, 
      Bethesda, MD, 20892-1184, USA.
FAU - Sabatino, Marianna
AU  - Sabatino M
AD  - Cell Processing Section, Department of Transfusion Medicine, Clinical Center, 
      National Institutes of Health, 10 Center Drive-MSC-1184, Building 10, Room 3C720, 
      Bethesda, MD, 20892-1184, USA.
FAU - Robey, Pamela G
AU  - Robey PG
AD  - Craniofacial and Skeletal Diseases Branch, National Institute of Dental and 
      Craniofacial Research, National Institutes of Health, 9000 Rockville Pike, 
      Bethesda, MD, 20892, USA.
FAU - Stroncek, David F
AU  - Stroncek DF
AUID- ORCID: 0000-0001-5867-3265
AD  - Cell Processing Section, Department of Transfusion Medicine, Clinical Center, 
      National Institutes of Health, 10 Center Drive-MSC-1184, Building 10, Room 3C720, 
      Bethesda, MD, 20892-1184, USA. dstroncek@cc.nih.gov.
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, N.I.H., Intramural
DEP - 20180314
PL  - England
TA  - J Transl Med
JT  - Journal of translational medicine
JID - 101190741
RN  - 0 (Biomarkers)
RN  - 0 (Cytokines)
RN  - 0 (MicroRNAs)
RN  - 0 (Platelet-Derived Growth Factor)
SB  - IM
MH  - Animals
MH  - Biomarkers/metabolism
MH  - Bone Marrow Cells/*cytology/drug effects
MH  - Cattle
MH  - Cell Culture Techniques/*methods
MH  - Cell Proliferation/drug effects
MH  - Cells, Cultured
MH  - Cytokines/metabolism
MH  - Gene Expression Regulation/drug effects
MH  - Humans
MH  - Lymphocyte Culture Test, Mixed
MH  - MicroRNAs/genetics/metabolism
MH  - Platelet-Derived Growth Factor/*pharmacology
MH  - Serum/*metabolism
MH  - Stromal Cells/cytology/drug effects
MH  - Transcriptome/genetics
PMC - PMC5853093
OTO - NOTNLM
OT  - Bone marrow stromal cells
OT  - Fetal bovine serum
OT  - Mesenchymal stem cells
OT  - Platelet lysate
OT  - Potency
EDAT- 2018/03/16 06:00
MHDA- 2019/06/14 06:00
PMCR- 2018/03/14
CRDT- 2018/03/16 06:00
PHST- 2017/12/20 00:00 [received]
PHST- 2018/02/01 00:00 [accepted]
PHST- 2018/03/16 06:00 [entrez]
PHST- 2018/03/16 06:00 [pubmed]
PHST- 2019/06/14 06:00 [medline]
PHST- 2018/03/14 00:00 [pmc-release]
AID - 10.1186/s12967-018-1400-3 [pii]
AID - 1400 [pii]
AID - 10.1186/s12967-018-1400-3 [doi]
PST - epublish
SO  - J Transl Med. 2018 Mar 14;16(1):65. doi: 10.1186/s12967-018-1400-3.