PMID- 29541084 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20201001 IS - 1664-462X (Print) IS - 1664-462X (Electronic) IS - 1664-462X (Linking) VI - 9 DP - 2018 TI - Transcriptomic Analysis Reveal the Molecular Mechanisms of Wheat Higher-Temperature Seedling-Plant Resistance to Puccinia striiformis f. sp. tritici. PG - 240 LID - 10.3389/fpls.2018.00240 [doi] LID - 240 AB - Stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is a destructive disease of wheat worldwide. The disease is preferably controlled by growing resistant cultivars. Wheat cultivar Xiaoyan 6 (XY 6) has been resistant to stripe rust since its release. In the previous studies, XY 6 was found to have higher-temperature seedling-plant (HTSP) resistance. However, the molecular mechanisms of HTSP resistance were not clear. To identify differentially expressed genes (DEGs) involved in HTSP resistance, we sequenced 30 cDNA libraries constructed from XY 6 seedlings exposed to several temperature treatments. Compared to the constant normal (15 degrees C) and higher (20 degrees C) temperature treatments, 1395 DEGs were identified in seedlings exposed to 20 degrees C for 24 h (to activate HTSP resistance) and then kept at 15 degrees C. These DEGs were located on all 21 chromosomes, with 29.2% on A, 41.1% on B and 29.7% on D genomes, by mapping to the Chinese Spring wheat genome. The 1395 DEGs were enriched in ribosome, plant-pathogen interaction and glycerolipid metabolism pathways, and some of them were identified as hub proteins (phosphatase 2C10), resistance protein homologs, WRKY transcription factors and protein kinases. The majority of these genes were up-regulated in HTSP resistance. Based on the differential expression, we found that phosphatase 2C10 and LRR receptor-like serine/threonine protein kinases are particularly interesting as they may be important for HTSP resistance through interacting with different resistance proteins, leading to a hypersensitive response. FAU - Tao, Fei AU - Tao F AD - State Key Laboratory of Crop Stress Biology for Arid Areas, College of Plant Protection, Northwest A&F University, Yangling, China. FAU - Wang, Junjuan AU - Wang J AD - State Key Laboratory of Crop Stress Biology for Arid Areas, College of Plant Protection, Northwest A&F University, Yangling, China. FAU - Guo, Zhongfeng AU - Guo Z AD - State Key Laboratory of Crop Stress Biology for Arid Areas, College of Plant Protection, Northwest A&F University, Yangling, China. FAU - Hu, Jingjing AU - Hu J AD - Wuhan UnigueGene Bioinformatics Science and Technology Co., Ltd, Wuhan, China. FAU - Xu, Xiangming AU - Xu X AD - NIAB East Malling Research (EMR), East Malling, United Kingdom. FAU - Yang, Jiarong AU - Yang J AD - State Key Laboratory of Crop Stress Biology for Arid Areas, College of Plant Protection, Northwest A&F University, Yangling, China. FAU - Chen, Xianming AU - Chen X AD - Agricultural Research Service, United States Department of Agriculture and Department of Plant Pathology, Washington State University, Pullman, WA, United States. FAU - Hu, Xiaoping AU - Hu X AD - State Key Laboratory of Crop Stress Biology for Arid Areas, College of Plant Protection, Northwest A&F University, Yangling, China. LA - eng PT - Journal Article DEP - 20180228 PL - Switzerland TA - Front Plant Sci JT - Frontiers in plant science JID - 101568200 PMC - PMC5835723 OTO - NOTNLM OT - Puccinia striiformis f. sp. tritici OT - higher temperature OT - non-race-specific resistance OT - plant defense OT - plant-pathogen interaction OT - transcript profiling OT - wheat EDAT- 2018/03/16 06:00 MHDA- 2018/03/16 06:01 PMCR- 2018/01/01 CRDT- 2018/03/16 06:00 PHST- 2017/10/11 00:00 [received] PHST- 2018/02/09 00:00 [accepted] PHST- 2018/03/16 06:00 [entrez] PHST- 2018/03/16 06:00 [pubmed] PHST- 2018/03/16 06:01 [medline] PHST- 2018/01/01 00:00 [pmc-release] AID - 10.3389/fpls.2018.00240 [doi] PST - epublish SO - Front Plant Sci. 2018 Feb 28;9:240. doi: 10.3389/fpls.2018.00240. eCollection 2018.