PMID- 29541390
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20191120
IS  - 1949-2553 (Electronic)
IS  - 1949-2553 (Linking)
VI  - 9
IP  - 13
DP  - 2018 Feb 16
TI  - Genomic BCR-ABL1 breakpoint characterization by a multi-strategy approach for 
      "personalized monitoring" of residual disease in chronic myeloid leukemia 
      patients.
PG  - 10978-10986
LID - 10.18632/oncotarget.23971 [doi]
AB  - For monitoring minimal residual disease (MRD) in chronic myeloid leukemia (CML) 
      the most recommended method is quantitative RT-PCR (RT-qPCR) for measuring 
      BCR-ABL1 transcripts. Several studies reported that a DNA-based assay enhances 
      the sensitivity of detection of the BCR-ABL1 genomic rearrangement, even if its 
      characterization results difficult. We developed a DNA-based method for detecting 
      and quantifying residual BCR-ABL1 positive leukemic stem cells in CML patients. 
      We propose two alternative approaches: the first one is a fluorescence in situ 
      hybridization (FISH)-based step followed by Sanger sequencing; the second one 
      employs MinION, a single molecule sequencer based on nanopore technology. 
      Finally, after defining the BCR-ABL1 genomic junction, we performed the target 
      CML patient-specific quantification, using droplet digital PCR (ddPCR). FISH and 
      MinION steps, respectively, together with ddPCR analysis, greatly reduce the 
      complexity that has impeded the use of "personalized monitoring" of CML in 
      clinical practice. Our report suggests a feasible pipeline, in terms of costs and 
      reproducibility, aimed at characterizing and quantifying the genomic BCR-ABL1 
      rearrangement during MRD monitoring in CML patients.
FAU - Cumbo, Cosimo
AU  - Cumbo C
AD  - Department of Emergency and Organ Transplantation, Hematology Section, University 
      of Bari, 70124 Bari, Italy.
FAU - Impera, Luciana
AU  - Impera L
AD  - Department of Emergency and Organ Transplantation, Hematology Section, University 
      of Bari, 70124 Bari, Italy.
FAU - Minervini, Crescenzio Francesco
AU  - Minervini CF
AD  - Department of Emergency and Organ Transplantation, Hematology Section, University 
      of Bari, 70124 Bari, Italy.
FAU - Orsini, Paola
AU  - Orsini P
AD  - Department of Emergency and Organ Transplantation, Hematology Section, University 
      of Bari, 70124 Bari, Italy.
FAU - Anelli, Luisa
AU  - Anelli L
AD  - Department of Emergency and Organ Transplantation, Hematology Section, University 
      of Bari, 70124 Bari, Italy.
FAU - Zagaria, Antonella
AU  - Zagaria A
AD  - Department of Emergency and Organ Transplantation, Hematology Section, University 
      of Bari, 70124 Bari, Italy.
FAU - Coccaro, Nicoletta
AU  - Coccaro N
AD  - Department of Emergency and Organ Transplantation, Hematology Section, University 
      of Bari, 70124 Bari, Italy.
FAU - Tota, Giuseppina
AU  - Tota G
AD  - Department of Emergency and Organ Transplantation, Hematology Section, University 
      of Bari, 70124 Bari, Italy.
FAU - Minervini, Angela
AU  - Minervini A
AD  - Department of Emergency and Organ Transplantation, Hematology Section, University 
      of Bari, 70124 Bari, Italy.
FAU - Casieri, Paola
AU  - Casieri P
AD  - Department of Emergency and Organ Transplantation, Hematology Section, University 
      of Bari, 70124 Bari, Italy.
FAU - Brunetti, Claudia
AU  - Brunetti C
AD  - Department of Emergency and Organ Transplantation, Hematology Section, University 
      of Bari, 70124 Bari, Italy.
FAU - Rossi, Antonella Russo
AU  - Rossi AR
AD  - Department of Emergency and Organ Transplantation, Hematology Section, University 
      of Bari, 70124 Bari, Italy.
FAU - Parciante, Elisa
AU  - Parciante E
AD  - Department of Emergency and Organ Transplantation, Hematology Section, University 
      of Bari, 70124 Bari, Italy.
FAU - Specchia, Giorgina
AU  - Specchia G
AD  - Department of Emergency and Organ Transplantation, Hematology Section, University 
      of Bari, 70124 Bari, Italy.
FAU - Albano, Francesco
AU  - Albano F
AD  - Department of Emergency and Organ Transplantation, Hematology Section, University 
      of Bari, 70124 Bari, Italy.
LA  - eng
PT  - Journal Article
DEP - 20180105
PL  - United States
TA  - Oncotarget
JT  - Oncotarget
JID - 101532965
PMC - PMC5834283
OTO - NOTNLM
OT  - FISH
OT  - MinION sequencing
OT  - chronic myeloid leukemia
OT  - droplet digital PCR
OT  - genomic BCR-ABL1 breakpoint
COIS- CONFLICTS OF INTEREST The authors declare no conflicts of interest.
EDAT- 2018/03/16 06:00
MHDA- 2018/03/16 06:01
PMCR- 2018/02/16
CRDT- 2018/03/16 06:00
PHST- 2017/11/13 00:00 [received]
PHST- 2018/01/02 00:00 [accepted]
PHST- 2018/03/16 06:00 [entrez]
PHST- 2018/03/16 06:00 [pubmed]
PHST- 2018/03/16 06:01 [medline]
PHST- 2018/02/16 00:00 [pmc-release]
AID - 23971 [pii]
AID - 10.18632/oncotarget.23971 [doi]
PST - epublish
SO  - Oncotarget. 2018 Jan 5;9(13):10978-10986. doi: 10.18632/oncotarget.23971. 
      eCollection 2018 Feb 16.