PMID- 29541628
OWN - NLM
STAT- MEDLINE
DCOM- 20190325
LR  - 20190325
IS  - 2235-2988 (Electronic)
IS  - 2235-2988 (Linking)
VI  - 8
DP  - 2018
TI  - A c-di-GMP-Modulating Protein Regulates Swimming Motility of Burkholderia 
      cenocepacia in Response to Arginine and Glutamate.
PG  - 56
LID - 10.3389/fcimb.2018.00056 [doi]
LID - 56
AB  - Burkholderia cenocepacia is an opportunistic bacterium that can thrive in 
      different environments, including the amino acid-rich mucus of the cystic 
      fibrosis (CF) lung. B. cenocepacia responds to the nutritional conditions that 
      mimic the CF sputum by increasing flagellin expression and swimming motility. 
      Individual amino acids also induce swimming but not flagellin expression. Here, 
      we show that modulation of the second messenger cyclic dimeric guanosine 
      monophosphate (c-di-GMP) levels by the PAS-containing c-di-GMP phosphodiesterase, 
      BCAL1069 (CdpA), regulates the swimming motility of B. cenocepacia K56-2 in 
      response to CF sputum nutritional conditions. Heterologous expression of WspR, a 
      diguanylate cyclase, in B. cenocepacia K56-2 caused an increase in c-di-GMP 
      levels and reduced swimming motility but did not affect flagellin expression or 
      flagellar biosynthesis. After insertional mutagenesis of 12 putative genes 
      encoding c-di-GMP metabolizing enzymes, one mutant of the locus BCAL1069 (cdpA), 
      exhibited decreased swimming motility independent of flagellin expression in CF 
      sputum nutritional conditions and an increase in intracellular c-di-GMP levels. 
      The reduced swimming motility phenotype of the BCAL1069 mutant was observed in 
      the presence of arginine and glutamate, but not of histidine, phenylalanine, or 
      proline. The B. cenocepacia CdpA was also found to be involved in regulation of 
      protease activity but not in biofilm formation. Altogether, these results 
      highlight a role of B. cenocepacia BCAL1069 (CdpA) in sensing the nutritional 
      conditions of the CF sputum and eliciting a pathogenic response that includes 
      swimming motility toward amino acids and an increase in protease activity.
FAU - Kumar, Brijesh
AU  - Kumar B
AD  - Department of Microbiology, Faculty of Science, University of Manitoba, Winnipeg, 
      MB, Canada.
FAU - Sorensen, John L
AU  - Sorensen JL
AD  - Department of Chemistry, Faculty of Science, University of Manitoba, Winnipeg, 
      MB, Canada.
FAU - Cardona, Silvia T
AU  - Cardona ST
AD  - Department of Microbiology, Faculty of Science, University of Manitoba, Winnipeg, 
      MB, Canada.
AD  - Department of Medical Microbiology & Infectious Diseases, Max Rady College of 
      Medicine, University of Manitoba, Winnipeg, MB, Canada.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180228
PL  - Switzerland
TA  - Front Cell Infect Microbiol
JT  - Frontiers in cellular and infection microbiology
JID - 101585359
RN  - 0 (Amino Acids)
RN  - 0 (Bacterial Proteins)
RN  - 0 (Escherichia coli Proteins)
RN  - 12777-81-0 (Flagellin)
RN  - 3KX376GY7L (Glutamic Acid)
RN  - 94ZLA3W45F (Arginine)
RN  - EC 3.1.4.- (Phosphoric Diester Hydrolases)
RN  - EC 3.4.- (Peptide Hydrolases)
RN  - EC 4.6.- (Phosphorus-Oxygen Lyases)
RN  - EC 4.6.1.- (diguanylate cyclase)
RN  - H2D2X058MU (Cyclic GMP)
SB  - IM
MH  - Amino Acid Sequence
MH  - Amino Acids/metabolism/pharmacology
MH  - Arginine/metabolism/*pharmacology
MH  - Bacterial Proteins/genetics/*metabolism
MH  - Biofilms/growth & development
MH  - Burkholderia Infections/microbiology
MH  - Burkholderia cenocepacia/*drug effects/genetics/*metabolism
MH  - Cloning, Molecular
MH  - Cyclic GMP/genetics/*metabolism
MH  - Cystic Fibrosis/metabolism/microbiology
MH  - Escherichia coli Proteins/metabolism
MH  - Flagellin/*biosynthesis
MH  - Gene Expression Regulation, Bacterial/drug effects
MH  - Glutamic Acid/metabolism/*pharmacology
MH  - Locomotion/drug effects
MH  - Lung/microbiology
MH  - Mutagenesis, Insertional
MH  - Peptide Hydrolases/metabolism
MH  - Phosphoric Diester Hydrolases/metabolism
MH  - Phosphorus-Oxygen Lyases/metabolism
MH  - Sequence Alignment
MH  - Sputum/chemistry
PMC - PMC5835511
OTO - NOTNLM
OT  - BCAL1069
OT  - Burkholderia cenocepacia
OT  - Burkholderia cepacia complex
OT  - SCFM
OT  - c-di-GMP
OT  - cdpA
OT  - cystic fibrosis
OT  - motility
EDAT- 2018/03/16 06:00
MHDA- 2019/03/26 06:00
PMCR- 2018/01/01
CRDT- 2018/03/16 06:00
PHST- 2017/11/12 00:00 [received]
PHST- 2018/02/12 00:00 [accepted]
PHST- 2018/03/16 06:00 [entrez]
PHST- 2018/03/16 06:00 [pubmed]
PHST- 2019/03/26 06:00 [medline]
PHST- 2018/01/01 00:00 [pmc-release]
AID - 10.3389/fcimb.2018.00056 [doi]
PST - epublish
SO  - Front Cell Infect Microbiol. 2018 Feb 28;8:56. doi: 10.3389/fcimb.2018.00056. 
      eCollection 2018.