PMID- 29541843
OWN - NLM
STAT- MEDLINE
DCOM- 20181105
LR  - 20181114
IS  - 1615-6102 (Electronic)
IS  - 0033-183X (Print)
IS  - 0033-183X (Linking)
VI  - 255
IP  - 5
DP  - 2018 Sep
TI  - FISH-aimed karyotype analysis in Aconitum subgen. Aconitum reveals excessive rDNA 
      sites in tetraploid taxa.
PG  - 1363-1372
LID - 10.1007/s00709-018-1238-9 [doi]
AB  - The location of 5S and 35S rDNA sequences in chromosomes of four Aconitum subsp. 
      Aconitum species was analyzed after fluorescence in situ hybridization (FISH). 
      Both in diploids (2n = 2x = 16; Aconitum variegatum, A. degenii) and tetraploids 
      (2n = 4x = 32; A. firmum, A. plicatum), rDNA repeats were localized exclusively 
      on the shorter arms of chromosomes, in subterminal or pericentromeric sites. All 
      analyzed species showed similar basal genome size (Cx = 5.31-5.71 pg). The most 
      striking features of tetraploid karyotypes were the conservation of diploid rDNA 
      loci and emergence of many additional 5S rDNA clusters. Chromosomal distribution 
      of excessive ribosomal sites suggests their role in the secondary diploidization 
      of tetraploid karyotypes.
FAU - Joachimiak, Andrzej J
AU  - Joachimiak AJ
AUID- ORCID: 0000-0002-6939-0938
AD  - Department of Plant Cytology and Embryology, Institute of Botany, Jagiellonian 
      University, Gronostajowa 9, PL-30-387, Krakow, Poland. a.joachimiak@uj.edu.pl.
FAU - Hasterok, Robert
AU  - Hasterok R
AD  - Department of Plant Anatomy and Cytology, University of Silesia in Katowice, 
      Jagiellonska 28, 40-032, Katowice, Poland.
FAU - Sliwinska, Elwira
AU  - Sliwinska E
AD  - Laboratory of Molecular Biology and Cytometry, Department of Plant Genetics and 
      Biotechnology, University of Technology and Life Sciences in Bydgoszcz, 
      Kaliskiego 7, 85-789, Bydgoszcz, Poland.
FAU - Musial, Krystyna
AU  - Musial K
AD  - Department of Plant Cytology and Embryology, Institute of Botany, Jagiellonian 
      University, Gronostajowa 9, PL-30-387, Krakow, Poland.
FAU - Grabowska-Joachimiak, Aleksandra
AU  - Grabowska-Joachimiak A
AD  - Department of Plant Breeding and Seed Science, University of Agriculture in 
      Krakow, Lobzowska 24, 31-140, Krakow, Poland.
LA  - eng
GR  - 2 P04C 072 29/State Commitee for Scientific Research/
PT  - Journal Article
DEP - 20180314
PL  - Austria
TA  - Protoplasma
JT  - Protoplasma
JID - 9806853
RN  - 0 (DNA, Plant)
RN  - 0 (DNA, Ribosomal)
SB  - IM
MH  - Aconitum/*genetics
MH  - Chromosomes, Plant/genetics
MH  - DNA, Plant/genetics
MH  - DNA, Ribosomal/genetics
MH  - Diploidy
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Karyotyping/*methods
MH  - Polyploidy
MH  - *Tetraploidy
PMC - PMC6133112
OTO - NOTNLM
OT  - Aconitum
OT  - Chromosomes
OT  - Diploidization
OT  - FISH
OT  - Nuclear DNA amount
OT  - rDNA
COIS- The authors declare that they have no conflict of interest.
EDAT- 2018/03/16 06:00
MHDA- 2018/11/06 06:00
PMCR- 2018/03/14
CRDT- 2018/03/16 06:00
PHST- 2017/11/18 00:00 [received]
PHST- 2018/03/05 00:00 [accepted]
PHST- 2018/03/16 06:00 [pubmed]
PHST- 2018/11/06 06:00 [medline]
PHST- 2018/03/16 06:00 [entrez]
PHST- 2018/03/14 00:00 [pmc-release]
AID - 10.1007/s00709-018-1238-9 [pii]
AID - 1238 [pii]
AID - 10.1007/s00709-018-1238-9 [doi]
PST - ppublish
SO  - Protoplasma. 2018 Sep;255(5):1363-1372. doi: 10.1007/s00709-018-1238-9. Epub 2018 
      Mar 14.