PMID- 29542183
OWN - NLM
STAT- MEDLINE
DCOM- 20181001
LR  - 20181001
IS  - 1439-0531 (Electronic)
IS  - 0936-6768 (Linking)
VI  - 53
IP  - 3
DP  - 2018 Jun
TI  - Endocannabinoids as endometrial inflammatory markers in lactating Holstein cows.
PG  - 769-775
LID - 10.1111/rda.13169 [doi]
AB  - The objective of this study was to consider endocannabinoid system as 
      inflammatory markers in bovine endometrium to better understand the role of this 
      system in regulating many of the functions that are related to inflammatory 
      condition. At day 26 post-partum, fourteen cows were divided into two groups 
      depending on the inflammatory condition: 1- subclinical endometritis (n = 7, with 
      purulent or mucopurulent uterine discharge detectable in the vagina) and 2- 
      healthy (n = 7, No (muco)) purulent discharge. Blood samples were collected at 26 
      and 30 days relative to calving to determine plasma tumour necrosis factor (TNF) 
      and lipopolysaccharide-binding protein (LBP) concentrations; moreover, uterine 
      biopsy was carried out on day 26 post-partum to measure mRNA abundance of TNF, 
      interleukin-1B (IL1B), interleukin-6 (IL-6), C-X-C motif chemokine ligand 8 
      (CXCL8), endocannabinoid receptor (CNR2), N-acyl phosphatidylethanolamine 
      phospholipase D (NAPEPLD), fatty acid amide hydrolase (FAAH), N-acylethanolamine 
      acid amidase (NAAA) and monoglyceride lipase (MGLL) by real-time PCR. Results 
      showed mean plasma concentrations of TNF and LBP were lower in healthy cows 
      compared to subclinical endometritis cows (p < .05). Relative mRNA expression for 
      NAAA and FAAH was decreased (p < .05), and relative mRNA expression for CNR2 and 
      NAPEPLD increased in cows with subclinical endometritis compared to healthy cows. 
      In conclusion, relative mRNA expression of TNF, IL1B and CXCL8 and plasma 
      concentration of LBP increased during inflammatory condition along with decreased 
      endocannabinoids hydrolyzing enzyme (NAAA and FAAH), increased enzymes that 
      synthesize endocannabinoids (NAPEPLD) and relative gene expression of the 
      endocannabinoid receptor; together, these contribute to increased 
      endocannabinoids levels during inflammation. Overall, we provide evidence that 
      endocannabinoid system is altered in endometrium tissue during inflammation 
      through increased mRNA expression of CNR2 and synthesis enzyme and decreased mRNA 
      expression of hydrolyzing enzymes interfere with pro-cytokine production and 
      signalling, which may interfere with the onset and progression of inflammation.
CI  - (c) 2018 Blackwell Verlag GmbH.
FAU - Bonsale, R
AU  - Bonsale R
AD  - Department of Animal Sciences, College of Agricultural Sciences and Natural 
      Resources, University of Mohaghegh Ardabili, Ardabil, Iran.
FAU - Seyed Sharifi, R
AU  - Seyed Sharifi R
AD  - Department of Animal Sciences, College of Agricultural Sciences and Natural 
      Resources, University of Mohaghegh Ardabili, Ardabil, Iran.
FAU - Dirandeh, E
AU  - Dirandeh E
AD  - Department of Animal Sciences, Sari Agricultural Sciences and Natural Resources 
      University, Sari, Iran.
FAU - Hedayat, N
AU  - Hedayat N
AD  - Department of Animal Sciences, College of Agricultural Sciences and Natural 
      Resources, University of Mohaghegh Ardabili, Ardabil, Iran.
FAU - Mojtahedin, A
AU  - Mojtahedin A
AD  - Department of Animal Sciences, College of Agricultural Sciences and Natural 
      Resources, University of Mohaghegh Ardabili, Ardabil, Iran.
FAU - Ghorbanalinia, M
AU  - Ghorbanalinia M
AD  - Department of Animal Sciences, Sari Agricultural Sciences and Natural Resources 
      University, Sari, Iran.
FAU - Abolghasemi, A
AU  - Abolghasemi A
AUID- ORCID: 0000-0003-0732-2996
AD  - Section of Physiology, Department of Biomedical Sciences, University of Cagliari, 
      Cagliari, Sardegna, Italy.
LA  - eng
PT  - Journal Article
DEP - 20180314
PL  - Germany
TA  - Reprod Domest Anim
JT  - Reproduction in domestic animals = Zuchthygiene
JID - 9015668
RN  - 0 (Acute-Phase Proteins)
RN  - 0 (Carrier Proteins)
RN  - 0 (Cytokines)
RN  - 0 (Endocannabinoids)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Cannabinoid)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Acute-Phase Proteins/analysis
MH  - Animals
MH  - Carrier Proteins/blood
MH  - Cattle
MH  - Cattle Diseases/metabolism/*pathology
MH  - Cytokines/*metabolism
MH  - Endocannabinoids/genetics/*metabolism
MH  - Endometritis/metabolism/*pathology
MH  - Female
MH  - Gene Expression
MH  - Lactation
MH  - RNA, Messenger/metabolism
MH  - Receptors, Cannabinoid/analysis
MH  - Tumor Necrosis Factor-alpha/blood
MH  - Uterus/enzymology/metabolism
OTO - NOTNLM
OT  - cytokine
OT  - dairy cow
OT  - endocannabinoids
OT  - inflammation
OT  - real-time PCR
EDAT- 2018/03/16 06:00
MHDA- 2018/10/03 06:00
CRDT- 2018/03/16 06:00
PHST- 2017/11/14 00:00 [received]
PHST- 2018/01/25 00:00 [accepted]
PHST- 2018/03/16 06:00 [pubmed]
PHST- 2018/10/03 06:00 [medline]
PHST- 2018/03/16 06:00 [entrez]
AID - 10.1111/rda.13169 [doi]
PST - ppublish
SO  - Reprod Domest Anim. 2018 Jun;53(3):769-775. doi: 10.1111/rda.13169. Epub 2018 Mar 
      14.