PMID- 29542193
OWN - NLM
STAT- MEDLINE
DCOM- 20181001
LR  - 20181001
IS  - 1439-0531 (Electronic)
IS  - 0936-6768 (Linking)
VI  - 53
IP  - 3
DP  - 2018 Jun
TI  - Hepatic steroid inactivating enzymes, hepatic portal blood flow and corpus luteum 
      blood perfusion in cattle.
PG  - 751-758
LID - 10.1111/rda.13166 [doi]
AB  - Production from the corpus luteum (CL) and/or hepatic steroid inactivation 
      impacts peripheral concentrations of P4, which can alter reproductive 
      performance. Our primary objective was to examine hepatic steroid inactivating 
      enzymes, portal blood flow, and luteal blood perfusion at 10 days 
      post-insemination in pregnant versus non-pregnant beef and dairy cows. Twenty 
      early lactation Holstein cows and 20 lactating commercial beef cows were utilized 
      for this study. At day 10 post-insemination, hepatic portal blood flow and CL 
      blood perfusion were measured via Doppler ultrasonography. Liver biopsies were 
      collected and frozen for later determination of cytochrome P450 1A (CYP1A), 2C 
      (CYP2C), 3A (CYP3A), uridine diphosphate-glucuronosyltransferase (UGT) and 
      aldo-keto reductase 1C (AKR1C) activities. Pregnancy was determined at day 30 
      post-insemination and treatment groups were retrospectively assigned as pregnant 
      or non-pregnant. Data were analyzed using the mixed procedure of SAS. Steroid 
      metabolizing enzyme activity was not different (p > .10) between pregnant versus 
      non-pregnant beef or dairy cows. Hepatic portal blood flow tended (p < .10) to be 
      increased in pregnant versus non-pregnant dairy cows. Luteal blood perfusion was 
      increased (p < .05) in pregnant versus non-pregnant dairy cows. Pregnant dairy 
      cows appear to have an increased rate of hepatic clearance of P4 in combination 
      with increased synthesis from the CL. This could account for the lack of 
      difference in peripheral P4 concentrations between pregnant and non-pregnant 
      dairy cows. This study highlights the relevance of further investigation into 
      steroid secretion and inactivation and their impact on the maintenance of 
      pregnancy in cattle.
CI  - (c) 2018 Blackwell Verlag GmbH.
FAU - Hart, C G
AU  - Hart CG
AD  - Department of Animal and Dairy Sciences, Mississippi State University, 
      Mississippi State, MS, USA.
FAU - Voelz, B E
AU  - Voelz BE
AD  - Department of Animal and Dairy Sciences, Mississippi State University, 
      Mississippi State, MS, USA.
FAU - Brockus, K E
AU  - Brockus KE
AD  - Department of Animal and Dairy Sciences, Mississippi State University, 
      Mississippi State, MS, USA.
FAU - Lemley, C O
AU  - Lemley CO
AUID- ORCID: 0000-0003-1377-0664
AD  - Department of Animal and Dairy Sciences, Mississippi State University, 
      Mississippi State, MS, USA.
LA  - eng
PT  - Comparative Study
PT  - Journal Article
DEP - 20180314
PL  - Germany
TA  - Reprod Domest Anim
JT  - Reproduction in domestic animals = Zuchthygiene
JID - 9015668
RN  - 4G7DS2Q64Y (Progesterone)
SB  - IM
MH  - Animals
MH  - Cattle
MH  - Corpus Luteum/*blood supply
MH  - Female
MH  - Lactation
MH  - Liver/*enzymology/metabolism
MH  - Liver Circulation/physiology
MH  - Portal Vein
MH  - Pregnancy/*physiology
MH  - Progesterone/metabolism
MH  - Ultrasonography, Doppler/veterinary
OTO - NOTNLM
OT  - cattle
OT  - corpus luteum
OT  - cytochrome P450
OT  - pregnancy
OT  - progesterone
EDAT- 2018/03/16 06:00
MHDA- 2018/10/03 06:00
CRDT- 2018/03/16 06:00
PHST- 2017/12/21 00:00 [received]
PHST- 2018/01/26 00:00 [accepted]
PHST- 2018/03/16 06:00 [pubmed]
PHST- 2018/10/03 06:00 [medline]
PHST- 2018/03/16 06:00 [entrez]
AID - 10.1111/rda.13166 [doi]
PST - ppublish
SO  - Reprod Domest Anim. 2018 Jun;53(3):751-758. doi: 10.1111/rda.13166. Epub 2018 Mar 
      14.