PMID- 29544080
OWN - NLM
STAT- MEDLINE
DCOM- 20181108
LR  - 20210224
IS  - 1480-3321 (Electronic)
IS  - 0831-2796 (Linking)
VI  - 61
IP  - 6
DP  - 2018 Jun
TI  - Identification and comparison of individual chromosomes of three accessions of 
      Hordeum chilense, Hordeum vulgare, and Triticum aestivum by FISH.
PG  - 387-396
LID - 10.1139/gen-2018-0016 [doi]
AB  - Karyotypes of three accessions of Hordeum chilense (H1, H16, and H7), Hordeum 
      vulgare, and Triticum aestivum were characterized by physical mapping of several 
      repetitive sequences. A total of 14 repetitive sequences were used as probes for 
      fluorescence in situ hybridization (FISH) with the aim of identifying inter- and 
      intraspecies polymorphisms. The (AG)(12) and 4P6 probes only produced 
      hybridization signals in wheat, the BAC7 probe only hybridized to the centromeric 
      region of H. vulgare, and the pSc119.2 probe hybridized to both wheat and H. 
      chilense, but not to H. vulgare. The remaining repetitive sequences used in this 
      study produced a hybridization signal in all the genotypes. Probes pAs1, pTa-535, 
      pTa71, CCS1, and CRW were much conserved, showing no significant polymorphism 
      among the genotypes studied. Probes GAA, (AAC)(5), (CTA)(5), HvT01, and pTa794 
      produced the most different hybridization pattern. We identified large 
      polymorphisms in the three accessions of H. chilense studied, supporting the 
      proposal of the existence of different groups inside species of H. chilense. The 
      set of probes described in this work allowed the identification of every single 
      chromosome in all three species, providing a complete cytogenetic karyotype of H. 
      chilense, H. vulgare, and T. aestivum chromosomes, which could be useful in wheat 
      and tritordeum breeding programs.
FAU - Rey, Maria-Dolores
AU  - Rey MD
AD  - John Innes Centre, Crop Genetics Department, Norwich NR4 7UH, United Kingdom.
AD  - John Innes Centre, Crop Genetics Department, Norwich NR4 7UH, United Kingdom.
FAU - Moore, Graham
AU  - Moore G
AD  - John Innes Centre, Crop Genetics Department, Norwich NR4 7UH, United Kingdom.
AD  - John Innes Centre, Crop Genetics Department, Norwich NR4 7UH, United Kingdom.
FAU - Martin, Azahara C
AU  - Martin AC
AD  - John Innes Centre, Crop Genetics Department, Norwich NR4 7UH, United Kingdom.
AD  - John Innes Centre, Crop Genetics Department, Norwich NR4 7UH, United Kingdom.
LA  - eng
GR  - BBS/E/J/00000315/BB_/Biotechnology and Biological Sciences Research 
      Council/United Kingdom
GR  - BBS/E/J/000PR9788/BB_/Biotechnology and Biological Sciences Research 
      Council/United Kingdom
PT  - Journal Article
DEP - 20180315
PL  - Canada
TA  - Genome
JT  - Genome
JID - 8704544
SB  - IM
MH  - Chromosomes, Plant/*genetics
MH  - Conserved Sequence
MH  - Genotype
MH  - Hordeum/*genetics
MH  - Karyotype
MH  - *Polymorphism, Genetic
MH  - Repetitive Sequences, Nucleic Acid
MH  - Triticum/*genetics
OTO - NOTNLM
OT  - FISH
OT  - Hordeum
OT  - Triticum aestivum
OT  - repetitive sequences
OT  - sequences repetees
OT  - tritordeum
EDAT- 2018/03/16 06:00
MHDA- 2018/11/09 06:00
CRDT- 2018/03/16 06:00
PHST- 2018/03/16 06:00 [pubmed]
PHST- 2018/11/09 06:00 [medline]
PHST- 2018/03/16 06:00 [entrez]
AID - 10.1139/gen-2018-0016 [doi]
PST - ppublish
SO  - Genome. 2018 Jun;61(6):387-396. doi: 10.1139/gen-2018-0016. Epub 2018 Mar 15.