PMID- 29544396
OWN - NLM
STAT- MEDLINE
DCOM- 20180911
LR  - 20200930
IS  - 1743-1328 (Electronic)
IS  - 0161-6412 (Linking)
VI  - 40
IP  - 5
DP  - 2018 May
TI  - Pitavastatin treatment induces neuroprotection through the BDNF-TrkB signalling 
      pathway in cultured cerebral neurons after oxygen-glucose deprivation.
PG  - 391-397
LID - 10.1080/01616412.2018.1447318 [doi]
AB  - OBJECTIVES: Along with their lipid-lowering effect, statins have been reported to 
      have neuroprotective function in both in vivo and in vitro models of 
      neurodegenerative diseases. We conducted this study in order to uncover the he 
      neuroprotective effect of the lipophilic statin pitavastatin (PTV) and 
      investigate the underlying molecular mechanisms using primary cultured cerebral 
      neurons exposed to oxygen-glucose deprivation (OGD). METHODS: The primary 
      cultured cerebral neurons were randomly assigned into four groups: the control 
      group, the pitavastatin treatment group, the OGD group and the OGD + pitavastatin 
      treatment group. The pitavastatin's concentration were set as follows: 1muM, 15muM, 
      30muM. After 3 hours OGD treatment, we use MTT method to assessment cell 
      viability, immunofluorescence to observe neuron morphology and western blot 
      method analysis the BDNF, TrkB. RESULTS: PTV at concentrations of 1 muM and 15 muM 
      elevated the survival rate of cortical neurons exposed to OGD, whereas 30 muM PTV 
      did not show such an effect. Moreover, PTV promoted neuronal dendrite growth at 
      concentrations of 1 muM and 15 muM. Increased expression levels of brain-derived 
      neurotrophic factor (BDNF) and tropomyosin-related kinase B (TrkB) were observed 
      in both of the following two scenarios: when neurons were treated with PTV for 48 
      hours and when PTV was added after the OGD procedure. CONCLUSION: Pitavastatin 
      treatment induces neuroprotection in cultured cerebral neurons after 
      oxygen-glucose deprivation this neuroprotection induced by PTV involves the 
      BDNF-TrkB signalling pathway.
FAU - Cui, Xiaoyan
AU  - Cui X
AD  - a Department of Neurology , The First Af fi liated Hospital of Zhengzhou University 
      , Zhengzhou , China.
FAU - Fu, Zhenqiang
AU  - Fu Z
AD  - a Department of Neurology , The First Af fi liated Hospital of Zhengzhou University 
      , Zhengzhou , China.
FAU - Wang, Menghan
AU  - Wang M
AD  - a Department of Neurology , The First Af fi liated Hospital of Zhengzhou University 
      , Zhengzhou , China.
FAU - Nan, Xiaofei
AU  - Nan X
AD  - c School of Information and Engineering , Zhengzhou University , Zhengzhou , 
      China.
FAU - Zhang, Boai
AU  - Zhang B
AD  - a Department of Neurology , The First Af fi liated Hospital of Zhengzhou University 
      , Zhengzhou , China.
AD  - b Institute of Clinical Medical Research , The First Affiliated Hospital of 
      Zhengzhou University , Zhengzhou , China.
LA  - eng
PT  - Journal Article
DEP - 20180316
PL  - England
TA  - Neurol Res
JT  - Neurological research
JID - 7905298
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Neuroprotective Agents)
RN  - 0 (Quinolines)
RN  - EC 2.7.10.1 (Ntrk2 protein, rat)
RN  - EC 2.7.10.1 (Receptor, trkB)
RN  - IY9XDZ35W2 (Glucose)
RN  - M5681Q5F9P (pitavastatin)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/metabolism
MH  - Cell Hypoxia/*drug effects/physiology
MH  - Cell Survival/drug effects/physiology
MH  - Cerebral Cortex/*drug effects/metabolism/pathology
MH  - Dose-Response Relationship, Drug
MH  - Glucose/*deficiency
MH  - Male
MH  - Neurons/*drug effects/metabolism/pathology
MH  - Neuroprotection/drug effects/physiology
MH  - Neuroprotective Agents/*pharmacology
MH  - Quinolines/*pharmacology
MH  - Random Allocation
MH  - Rats, Sprague-Dawley
MH  - Receptor, trkB/metabolism
MH  - Signal Transduction/drug effects
MH  - Time Factors
OTO - NOTNLM
OT  - BDNF-TrkB
OT  - Pitavastatin
OT  - cerebral cortex
OT  - neuroprotection
OT  - oxygen-glucose deprivation
EDAT- 2018/03/17 06:00
MHDA- 2018/09/12 06:00
CRDT- 2018/03/17 06:00
PHST- 2018/03/17 06:00 [pubmed]
PHST- 2018/09/12 06:00 [medline]
PHST- 2018/03/17 06:00 [entrez]
AID - 10.1080/01616412.2018.1447318 [doi]
PST - ppublish
SO  - Neurol Res. 2018 May;40(5):391-397. doi: 10.1080/01616412.2018.1447318. Epub 2018 
      Mar 16.