PMID- 29545847
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 1792-0981 (Print)
IS  - 1792-1015 (Electronic)
IS  - 1792-0981 (Linking)
VI  - 15
IP  - 4
DP  - 2018 Apr
TI  - Expression of DNA-dependent protein kinase catalytic subunit in laryngeal 
      squamous cell carcinoma and its importance.
PG  - 3295-3301
LID - 10.3892/etm.2018.5826 [doi]
AB  - The aim of the present study was to explore the expression and distribution of 
      DNA-dependent protein kinase catalytic subunit (DNA-PKcs) in tumor tissues and 
      adjacent normal mucosa tissues of patients with laryngeal squamous cell carcinoma 
      (LSCC), and further analyze the association between the expression and the 
      clinicopathological parameters of patients with LSCC. Clinical data of tumor 
      tissues and corresponding adjacent normal mucosa tissues of pathologically 
      diagnosed LSCC in 96 cases were collected in the present study. Of these 
      specimens, the mRNA and protein expression levels of DNA-PKcs in LSCC tissues and 
      the adjacent normal mucosa tissues were analyzed via reverse 
      transcription-quantitative polymerase chain reaction and western blot analysis. 
      Immunohistochemistry was used to detect expression and distribution of DNA-PKcs 
      protein in LSCC tissues and corresponding adjacent normal mucosa tissues. The 
      association between DNA-PKcs expression and the specific clinicopathologic 
      features was evaluated by the chi(2) test. Kaplan-Meier and Cox proportional 
      hazards regression models were used to analyze the data. It was revealed that the 
      expression of DNA-PKcs mRNA and protein was significantly higher in LSCC tissues 
      than the adjacent normal mucosa tissues (P<0.05). DNA-PKcs was expressed 
      predominantly in the nucleus. DNA-PKcs expression showed significant correlation 
      with the differentiation degree of LSCC (P<0.05), and changes of DNA-PKcs 
      expression gradually increased with the decrease of the differentiation degree. 
      However, DNA-PKcs expression was not significantly associated with sex, age, 
      lymph node metastasis or TMN stage (P>0.05). Patients with LSCC exhibited higher 
      DNA-PKcs expression had markedly shorter survival than those with lower DNA-PKcs 
      expression. In conclusion, the present results suggested that the expression 
      levels of DNA-PKcs were significantly increased in LSCC tumor tissues than in 
      adjacent normal mucosa. DNA-PKcs expression was correlated with differentiation 
      of LSCC, and may become a novel prognostic marker for patients with LSCC.
FAU - Sun, Jian-Song
AU  - Sun JS
AD  - Department of Otolaryngology-Head and Neck Surgery, The People's Hospital of 
      Guizhou Province, Guiyang, Guizhou 550002, P.R. China.
FAU - Yang, Xiu-Hai
AU  - Yang XH
AD  - Department of Otolaryngology-Head and Neck Surgery, The People's Hospital of 
      Guizhou Province, Guiyang, Guizhou 550002, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20180201
PL  - Greece
TA  - Exp Ther Med
JT  - Experimental and therapeutic medicine
JID - 101531947
PMC - PMC5840916
OTO - NOTNLM
OT  - DNA-dependent protein kinase catalytic subunit
OT  - laryngeal neoplasm
OT  - squamous cell carcinoma
EDAT- 2018/03/17 06:00
MHDA- 2018/03/17 06:01
PMCR- 2018/02/01
CRDT- 2018/03/17 06:00
PHST- 2016/07/05 00:00 [received]
PHST- 2017/06/08 00:00 [accepted]
PHST- 2018/03/17 06:00 [entrez]
PHST- 2018/03/17 06:00 [pubmed]
PHST- 2018/03/17 06:01 [medline]
PHST- 2018/02/01 00:00 [pmc-release]
AID - ETM-0-0-5826 [pii]
AID - 10.3892/etm.2018.5826 [doi]
PST - ppublish
SO  - Exp Ther Med. 2018 Apr;15(4):3295-3301. doi: 10.3892/etm.2018.5826. Epub 2018 Feb 
      1.