PMID- 29546036
OWN - NLM
STAT- MEDLINE
DCOM- 20190325
LR  - 20190325
IS  - 2235-2988 (Electronic)
IS  - 2235-2988 (Linking)
VI  - 8
DP  - 2018
TI  - The Conserved ESCRT-III Machinery Participates in the Phagocytosis of Entamoeba 
      histolytica.
PG  - 53
LID - 10.3389/fcimb.2018.00053 [doi]
LID - 53
AB  - The endosomal sorting complex required for transport (ESCRT) orchestrates cell 
      membrane-remodeling mechanisms in eukaryotes, including endocytosis. However, 
      ESCRT functions in phagocytosis (ingestion of >/=250 nm particles), has been poorly 
      studied. In macrophages and amoebae, phagocytosis is required for cell nutrition 
      and attack to other microorganisms and cells. In Entamoeba histolytica, the 
      voracious protozoan responsible for human amoebiasis, phagocytosis is a land mark 
      of virulence. Here, we have investigated the role of ESCRT-III in the 
      phagocytosis of E. histolytica, using mutant trophozoites, recombinant proteins 
      (rEhVps20, rEhVps32, rEhVps24, and rEhVps2) and giant unilamellar vesicles 
      (GUVs). Confocal images displayed the four proteins located around the ingested 
      erythrocytes, in erythrocytes-containing phagosomes and in multivesicular bodies. 
      EhVps32 and EhVps2 proteins co-localized at the phagocytic cups. Protein 
      association increased during phagocytosis. Immunoprecipitation and flow cytometry 
      assays substantiated these associations. GUVs revealed that the protein assembly 
      sequence is essential to form intraluminal vesicles (ILVs). First, the active 
      rEhVps20 bound to membranes and recruited rEhVps32, promoting membrane 
      invaginations. rEhVps24 allowed the detachment of nascent vesicles, forming ILVs; 
      and rEhVps2 modulated their size. The knock down of Ehvps20 and Ehvps24genes 
      diminished the rate of erythrophagocytosis demonstrating the importance of 
      ESCRT-III in this event. In conclusion, we present here evidence of the ESCRT-III 
      participation in phagocytosis and delimitate the putative function of proteins, 
      according to the in vitro reconstruction of their assembling.
FAU - Avalos-Padilla, Yunuen
AU  - Avalos-Padilla Y
AD  - Department of Theory and Bio-Systems, Max Planck Institute of Colloids and 
      Interfaces, Potsdam, Germany.
AD  - Departamento de Infectomica y Patogenesis Molecular, CINVESTAV IPN, Mexico City, 
      Mexico.
FAU - Knorr, Roland L
AU  - Knorr RL
AD  - Department of Theory and Bio-Systems, Max Planck Institute of Colloids and 
      Interfaces, Potsdam, Germany.
FAU - Javier-Reyna, Rosario
AU  - Javier-Reyna R
AD  - Departamento de Infectomica y Patogenesis Molecular, CINVESTAV IPN, Mexico City, 
      Mexico.
FAU - Garcia-Rivera, Guillermina
AU  - Garcia-Rivera G
AD  - Departamento de Infectomica y Patogenesis Molecular, CINVESTAV IPN, Mexico City, 
      Mexico.
FAU - Lipowsky, Reinhard
AU  - Lipowsky R
AD  - Department of Theory and Bio-Systems, Max Planck Institute of Colloids and 
      Interfaces, Potsdam, Germany.
FAU - Dimova, Rumiana
AU  - Dimova R
AD  - Department of Theory and Bio-Systems, Max Planck Institute of Colloids and 
      Interfaces, Potsdam, Germany.
FAU - Orozco, Esther
AU  - Orozco E
AD  - Departamento de Infectomica y Patogenesis Molecular, CINVESTAV IPN, Mexico City, 
      Mexico.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180301
PL  - Switzerland
TA  - Front Cell Infect Microbiol
JT  - Frontiers in cellular and infection microbiology
JID - 101585359
RN  - 0 (CHMP2A protein, human)
RN  - 0 (CHMP3 protein, human)
RN  - 0 (CHMP6 protein, human)
RN  - 0 (Endosomal Sorting Complexes Required for Transport)
RN  - 0 (Protozoan Proteins)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Saccharomyces cerevisiae Proteins)
RN  - 0 (Unilamellar Liposomes)
SB  - IM
MH  - Cell Membrane/metabolism
MH  - Endosomal Sorting Complexes Required for Transport/chemistry/genetics/*metabolism
MH  - Entamoeba histolytica/*metabolism/*pathogenicity
MH  - Erythrocytes
MH  - Flow Cytometry
MH  - Fluorescent Antibody Technique
MH  - Humans
MH  - Immunoprecipitation
MH  - Models, Molecular
MH  - Multivesicular Bodies/metabolism
MH  - Phagocytosis/*physiology
MH  - Phagosomes
MH  - Protozoan Proteins/metabolism
MH  - Recombinant Proteins/chemistry/genetics/metabolism
MH  - Saccharomyces cerevisiae Proteins/chemistry/genetics/metabolism
MH  - Trophozoites/cytology/genetics/*metabolism
MH  - Unilamellar Liposomes/metabolism
PMC - PMC5838018
OTO - NOTNLM
OT  - ESCRT-III proteins
OT  - Entamoeba histolytica
OT  - GUVs model
OT  - phagocytosis
OT  - protozoan parasites
EDAT- 2018/03/17 06:00
MHDA- 2019/03/26 06:00
PMCR- 2018/01/01
CRDT- 2018/03/17 06:00
PHST- 2017/12/11 00:00 [received]
PHST- 2018/02/12 00:00 [accepted]
PHST- 2018/03/17 06:00 [entrez]
PHST- 2018/03/17 06:00 [pubmed]
PHST- 2019/03/26 06:00 [medline]
PHST- 2018/01/01 00:00 [pmc-release]
AID - 10.3389/fcimb.2018.00053 [doi]
PST - epublish
SO  - Front Cell Infect Microbiol. 2018 Mar 1;8:53. doi: 10.3389/fcimb.2018.00053. 
      eCollection 2018.