PMID- 29547375
OWN - NLM
STAT- MEDLINE
DCOM- 20190122
LR  - 20190122
IS  - 1751-732X (Electronic)
IS  - 1751-7311 (Linking)
VI  - 12
IP  - 12
DP  - 2018 Dec
TI  - Promising perspectives for ruminal protection of polyunsaturated fatty acids 
      through polyphenol-oxidase-mediated crosslinking of interfacial protein in 
      emulsions.
PG  - 2539-2550
LID - 10.1017/S1751731118000423 [doi]
AB  - Previously, polyunsaturated fatty acids (PUFA) from linseed oil were effectively 
      protected (>80%) against biohydrogenation through polyphenol-oxidase-mediated 
      protein crosslinking of an emulsion, prepared with polyphenol oxidase (PPO) 
      extract from potato tuber peelings. However, until now, emulsions of only 2 wt% 
      oil have been successfully protected, which implies serious limitations both from 
      a research perspective (e.g. in vivo trials) as well as for further upscaling 
      toward practical applications. Therefore, the aim of this study was to increase 
      the oil/PPO ratio. In the original protocol, the PPO extract served both an 
      emulsifying function as well as a crosslinking function. Here, it was first 
      evaluated whether alternative protein sources could replace the emulsifying 
      function of the PPO extract, with addition of PPO extract and 4-methylcatechol 
      (4MC) to induce crosslinking after emulsion preparation. This approach was then 
      further used to evaluate protection of emulsions with higher oil content. Five 
      candidate emulsifiers (soy glycinin, gelatin, whey protein isolate (WPI), bovine 
      serum albumin and sodium caseinate) were used to prepare 10 wt% oil emulsions, 
      which were diluted five times (w/w) with PPO extract (experiment 1). As a 
      positive control, 2 wt% oil emulsions were prepared directly with PPO extract 
      according to the original protocol. Further, emulsions of 2, 4, 6, 8 and 10 wt% 
      oil were prepared, with 80 wt% PPO extract (experiment 2), or with 90, 80, 70, 60 
      and 50 wt% PPO extract, respectively (experiment 3) starting from WPI-stabilized 
      emulsions. Enzymatic crosslinking was induced by 24-h incubation with 4MC. 
      Ruminal protection efficiency was evaluated by 24-h in vitro batch simulation of 
      the rumen metabolism. In experiment 1, protection efficiencies were equal or 
      higher than the control (85.5% to 92.5% v. 81.3%). In both experiments 2 and 3, 
      high protection efficiencies (>80%) were achieved, except for emulsions 
      containing 10 wt% oil emulsions (<50% protection), which showed oiling-off after 
      enzymatic crosslinking. This study demonstrated that alternative emulsifier 
      proteins can be used in combination with PPO extract to protect emulsified 
      PUFA-rich oils against ruminal biohydrogenation. By applying the new protocol, 
      6.5 times less PPO extract was required.
FAU - De Neve, N
AU  - De Neve N
AD  - 1Laboratory for Animal Nutrition and Animal Product Quality,Department of Animal 
      Production,Ghent University,Coupure Links 653,Block F,9000 Ghent,Belgium.
FAU - Vlaeminck, B
AU  - Vlaeminck B
AD  - 1Laboratory for Animal Nutrition and Animal Product Quality,Department of Animal 
      Production,Ghent University,Coupure Links 653,Block F,9000 Ghent,Belgium.
FAU - Gadeyne, F
AU  - Gadeyne F
AD  - 1Laboratory for Animal Nutrition and Animal Product Quality,Department of Animal 
      Production,Ghent University,Coupure Links 653,Block F,9000 Ghent,Belgium.
FAU - Claeys, E
AU  - Claeys E
AD  - 1Laboratory for Animal Nutrition and Animal Product Quality,Department of Animal 
      Production,Ghent University,Coupure Links 653,Block F,9000 Ghent,Belgium.
FAU - Van der Meeren, P
AU  - Van der Meeren P
AD  - 2Particle and Interfacial Technology Group, Faculty of Bioscience 
      Engineering,Ghent University,Coupure Links 653,Block B,9000 Ghent,Belgium.
FAU - Fievez, V
AU  - Fievez V
AD  - 1Laboratory for Animal Nutrition and Animal Product Quality,Department of Animal 
      Production,Ghent University,Coupure Links 653,Block F,9000 Ghent,Belgium.
LA  - eng
PT  - Journal Article
DEP - 20180316
PL  - England
TA  - Animal
JT  - Animal : an international journal of animal bioscience
JID - 101303270
RN  - 0 (Cross-Linking Reagents)
RN  - 0 (Emulsifying Agents)
RN  - 0 (Emulsions)
RN  - 0 (Fatty Acids, Unsaturated)
RN  - 0 (Plant Proteins)
RN  - 8001-26-1 (Linseed Oil)
RN  - EC 1.10.3.1 (Catechol Oxidase)
SB  - IM
MH  - Animals
MH  - Catechol Oxidase/*chemistry
MH  - Cross-Linking Reagents/chemistry
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Emulsifying Agents/*chemistry
MH  - Emulsions/chemistry
MH  - Fatty Acids, Unsaturated/*chemistry
MH  - Linseed Oil/*chemistry
MH  - Oxidation-Reduction
MH  - Plant Proteins/chemistry
MH  - Plant Tubers/enzymology
MH  - Rumen/metabolism
MH  - Solanum tuberosum/*enzymology
OTO - NOTNLM
OT  - in vitro
OT  - rumen-bypass
OT  - sodium dodecyl sulfate polyacrylamide gel electrophoresis
OT  - tyrosinase
OT  - zymography
EDAT- 2018/03/17 06:00
MHDA- 2019/01/23 06:00
CRDT- 2018/03/17 06:00
PHST- 2018/03/17 06:00 [pubmed]
PHST- 2019/01/23 06:00 [medline]
PHST- 2018/03/17 06:00 [entrez]
AID - S1751731118000423 [pii]
AID - 10.1017/S1751731118000423 [doi]
PST - ppublish
SO  - Animal. 2018 Dec;12(12):2539-2550. doi: 10.1017/S1751731118000423. Epub 2018 Mar 
      16.