PMID- 29547589
OWN - NLM
STAT- MEDLINE
DCOM- 20180904
LR  - 20181114
IS  - 1660-3397 (Electronic)
IS  - 1660-3397 (Linking)
VI  - 16
IP  - 3
DP  - 2018 Mar 16
TI  - Phocoenamicins B and C, New Antibacterial Spirotetronates Isolated from a Marine 
      Micromonospora sp.
LID - 10.3390/md16030095 [doi]
LID - 95
AB  - Phocoenamicins B and C (1 and 2), together with the known spirotetronate 
      phocoenamicin (3), were isolated from cultures of Micromonospora sp. The acetone 
      extract from a culture of this strain, isolated from marine sediments collected 
      in the Canary Islands, displayed activity against methicillin-resistant 
      Staphylococcus aureus (MRSA), Mycobacterium tuberculosis H37Ra and Mycobacterium 
      bovis. Bioassay-guided fractionation of this extract using SP207ss column 
      chromatography and preparative reversed-phased HPLC led to the isolation of the 
      new compounds 1 and 2 belonging to the spirotetronate class of polyketides. Their 
      structures were determined using a combination of HRMS, 1D and 2D NMR experiments 
      and comparison with the spectra reported for phocoenamicin. Antibacterial 
      activity tests of the pure compounds against these pathogens revealed minimal 
      inhibitory concentration (MIC) values ranging from 4 to 64 microg/mL for MRSA, and 16 
      to 32 microg/mL for M. tuberculosis H37Ra, with no significant activity found against 
      M. bovis and vancomycin-resistant Enterococcus faecium (VRE) at concentrations 
      below 128 microg/mL, and weak activity detected against Bacillus subtilis grown on 
      agar plates.
FAU - Perez-Bonilla, Mercedes
AU  - Perez-Bonilla M
AD  - Fundacion MEDINA, Centro de Excelencia en Investigacion de Medicamentos 
      Innovadores en Andalucia, Parque Tecnologico Ciencias de la Salud, Avda. del 
      Conocimiento 34, 18016 Armilla, Granada, Spain. 
      mercedes.perez@medinaandalucia.es.
FAU - Oves-Costales, Daniel
AU  - Oves-Costales D
AD  - Fundacion MEDINA, Centro de Excelencia en Investigacion de Medicamentos 
      Innovadores en Andalucia, Parque Tecnologico Ciencias de la Salud, Avda. del 
      Conocimiento 34, 18016 Armilla, Granada, Spain. daniel.oves@medinaandalucia.es.
FAU - de la Cruz, Mercedes
AU  - de la Cruz M
AUID- ORCID: 0000-0002-2389-8576
AD  - Fundacion MEDINA, Centro de Excelencia en Investigacion de Medicamentos 
      Innovadores en Andalucia, Parque Tecnologico Ciencias de la Salud, Avda. del 
      Conocimiento 34, 18016 Armilla, Granada, Spain. 
      mercedes.delacruz@medinaandalucia.es.
FAU - Kokkini, Maria
AU  - Kokkini M
AD  - Fundacion MEDINA, Centro de Excelencia en Investigacion de Medicamentos 
      Innovadores en Andalucia, Parque Tecnologico Ciencias de la Salud, Avda. del 
      Conocimiento 34, 18016 Armilla, Granada, Spain. maria.kokkini@medinandalucia.es.
FAU - Martin, Jesus
AU  - Martin J
AD  - Fundacion MEDINA, Centro de Excelencia en Investigacion de Medicamentos 
      Innovadores en Andalucia, Parque Tecnologico Ciencias de la Salud, Avda. del 
      Conocimiento 34, 18016 Armilla, Granada, Spain. jesus.martin@medinaandalucia.es.
FAU - Vicente, Francisca
AU  - Vicente F
AD  - Fundacion MEDINA, Centro de Excelencia en Investigacion de Medicamentos 
      Innovadores en Andalucia, Parque Tecnologico Ciencias de la Salud, Avda. del 
      Conocimiento 34, 18016 Armilla, Granada, Spain. 
      francisca.vicente@medinaandalucia.es.
FAU - Genilloud, Olga
AU  - Genilloud O
AD  - Fundacion MEDINA, Centro de Excelencia en Investigacion de Medicamentos 
      Innovadores en Andalucia, Parque Tecnologico Ciencias de la Salud, Avda. del 
      Conocimiento 34, 18016 Armilla, Granada, Spain. 
      olga.genilloud@medinaandalucia.es.
FAU - Reyes, Fernando
AU  - Reyes F
AUID- ORCID: 0000-0003-1607-5106
AD  - Fundacion MEDINA, Centro de Excelencia en Investigacion de Medicamentos 
      Innovadores en Andalucia, Parque Tecnologico Ciencias de la Salud, Avda. del 
      Conocimiento 34, 18016 Armilla, Granada, Spain. 
      fernando.reyes@medinaandalucia.es.
LA  - eng
PT  - Journal Article
DEP - 20180316
PL  - Switzerland
TA  - Mar Drugs
JT  - Marine drugs
JID - 101213729
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Biological Products)
SB  - IM
MH  - Anti-Bacterial Agents/*chemistry/pharmacology
MH  - Aquatic Organisms/*chemistry
MH  - Bacteria/drug effects
MH  - Biological Products/*chemistry/pharmacology
MH  - Geologic Sediments/microbiology
MH  - Magnetic Resonance Spectroscopy/methods
MH  - Micromonospora/*chemistry
MH  - Spain
PMC - PMC5867639
OTO - NOTNLM
OT  - Micromonospora sp.
OT  - antimicrobial activity
OT  - spirotetronates
OT  - structural elucidation
COIS- The authors declare no conflict of interest.
EDAT- 2018/03/17 06:00
MHDA- 2018/09/05 06:00
PMCR- 2018/03/01
CRDT- 2018/03/17 06:00
PHST- 2018/02/20 00:00 [received]
PHST- 2018/03/13 00:00 [revised]
PHST- 2018/03/14 00:00 [accepted]
PHST- 2018/03/17 06:00 [entrez]
PHST- 2018/03/17 06:00 [pubmed]
PHST- 2018/09/05 06:00 [medline]
PHST- 2018/03/01 00:00 [pmc-release]
AID - md16030095 [pii]
AID - marinedrugs-16-00095 [pii]
AID - 10.3390/md16030095 [doi]
PST - epublish
SO  - Mar Drugs. 2018 Mar 16;16(3):95. doi: 10.3390/md16030095.