PMID- 29549156
OWN - NLM
STAT- MEDLINE
DCOM- 20191014
LR  - 20191014
IS  - 1521-0103 (Electronic)
IS  - 0022-3565 (Linking)
VI  - 365
IP  - 3
DP  - 2018 Jun
TI  - Ultrasound Combined with Microbubbles Enhances the Effects of Methylprednisolone 
      in Lipopolysaccharide-Induced Human Mesangial Cells.
PG  - 476-484
LID - 10.1124/jpet.117.246223 [doi]
AB  - A novel drug delivery system mediated by ultrasound (US) combined with 
      microbubbles (MBs) (US+MB) could improve local drug concentration to enhance its 
      efficacy. To investigate the influence of US+MB on methylprednisolone (MP), the 
      effect of US+MB combined with MP (US+MB+MP) on lipopolysaccharide (LPS)-induced 
      human mesangial cells (HMCs) and the underlying mechanism were explored in this 
      study. The results revealed that HMCs treated with LPS underwent significant 
      proliferation and exhibited an increase in nuclear transcription factor-kappaB 
      (NF-kappaB) and transforming growth factor-beta1 (TGF-beta1) expression and a decrease in 
      cellular apoptosis. This effect was significantly inhibited by MP (30-100 mug/ml), 
      US combined with MBs (3.22 x 10(7) and 8.05 x 10(7) bubbles/ml), and US combined 
      with both MBs (1.29 x 10(7) bubbles/ml) and MP (12 mug/ml) (US+MB1+MP12). The 
      effect of US+MB1+MP12 was better than the effect of 12 mug/ml of MP alone and was 
      similar to the effect of 100 mug/ml of MP. Additionally, the intracellular free MP 
      content was significantly higher in the US+MB1+MP12 group than in the MP12 group. 
      US combined with MBs not only inhibited LPS-induced HMC proliferation and NF-kappaB 
      and TGF-beta1 expression and increased cellular apoptosis but also synergized with 
      the pharmacologic effect of MP. The mechanism is partially due to the US-assisted 
      MB local drug delivery and the anti-inflammatory effect induced by US combined 
      with MBs.
CI  - Copyright (c) 2018 by The American Society for Pharmacology and Experimental 
      Therapeutics.
FAU - Sun, Peng-Fei
AU  - Sun PF
AD  - Department of Pathology (P.-F.S., T.T., B.W., J.Z., S.-T.R.), Department of 
      Pharmacology (L.-N.C.), and Therapeutic Vaccines Engineering Center of Shaanxi 
      Province (B.W.), School of Basic Medical Sciences, Xi'an Jiaotong University 
      Health Science Center, Xi'an, People's Republic of China; Department of 
      Pathology, Xi'an City Center Hospital, Xi'an, People's Republic of China 
      (P.-F.S.); Department of Nephrology, Second Affiliated Hospital of Xi'an Jiaotong 
      University, Xi'an, People's Republic of China (R.-G.F.); Department of Biomedical 
      Engineering, School of Life Science and Technology, Xi'an Jiaotong University, 
      Xi'an, People's Republic of China (S.-S.X.); Department of Ultrasound, First 
      Affiliated Hospital of Xi'an Jiaotong University, Xi'an, People's Republic of 
      China (H.A.); Shanghai Medical College, Fudan University, Shanghai, People's 
      Republic of China (R.-Y.S.); and Department of Diabetes, Central Clinical School, 
      Monash University, Melbourne, Australia (Z.C., M.E.C.).
FAU - Tian, Tian
AU  - Tian T
AD  - Department of Pathology (P.-F.S., T.T., B.W., J.Z., S.-T.R.), Department of 
      Pharmacology (L.-N.C.), and Therapeutic Vaccines Engineering Center of Shaanxi 
      Province (B.W.), School of Basic Medical Sciences, Xi'an Jiaotong University 
      Health Science Center, Xi'an, People's Republic of China; Department of 
      Pathology, Xi'an City Center Hospital, Xi'an, People's Republic of China 
      (P.-F.S.); Department of Nephrology, Second Affiliated Hospital of Xi'an Jiaotong 
      University, Xi'an, People's Republic of China (R.-G.F.); Department of Biomedical 
      Engineering, School of Life Science and Technology, Xi'an Jiaotong University, 
      Xi'an, People's Republic of China (S.-S.X.); Department of Ultrasound, First 
      Affiliated Hospital of Xi'an Jiaotong University, Xi'an, People's Republic of 
      China (H.A.); Shanghai Medical College, Fudan University, Shanghai, People's 
      Republic of China (R.-Y.S.); and Department of Diabetes, Central Clinical School, 
      Monash University, Melbourne, Australia (Z.C., M.E.C.).
FAU - Chen, Li-Na
AU  - Chen LN
AD  - Department of Pathology (P.-F.S., T.T., B.W., J.Z., S.-T.R.), Department of 
      Pharmacology (L.-N.C.), and Therapeutic Vaccines Engineering Center of Shaanxi 
      Province (B.W.), School of Basic Medical Sciences, Xi'an Jiaotong University 
      Health Science Center, Xi'an, People's Republic of China; Department of 
      Pathology, Xi'an City Center Hospital, Xi'an, People's Republic of China 
      (P.-F.S.); Department of Nephrology, Second Affiliated Hospital of Xi'an Jiaotong 
      University, Xi'an, People's Republic of China (R.-G.F.); Department of Biomedical 
      Engineering, School of Life Science and Technology, Xi'an Jiaotong University, 
      Xi'an, People's Republic of China (S.-S.X.); Department of Ultrasound, First 
      Affiliated Hospital of Xi'an Jiaotong University, Xi'an, People's Republic of 
      China (H.A.); Shanghai Medical College, Fudan University, Shanghai, People's 
      Republic of China (R.-Y.S.); and Department of Diabetes, Central Clinical School, 
      Monash University, Melbourne, Australia (Z.C., M.E.C.).
FAU - Fu, Rong-Guo
AU  - Fu RG
AD  - Department of Pathology (P.-F.S., T.T., B.W., J.Z., S.-T.R.), Department of 
      Pharmacology (L.-N.C.), and Therapeutic Vaccines Engineering Center of Shaanxi 
      Province (B.W.), School of Basic Medical Sciences, Xi'an Jiaotong University 
      Health Science Center, Xi'an, People's Republic of China; Department of 
      Pathology, Xi'an City Center Hospital, Xi'an, People's Republic of China 
      (P.-F.S.); Department of Nephrology, Second Affiliated Hospital of Xi'an Jiaotong 
      University, Xi'an, People's Republic of China (R.-G.F.); Department of Biomedical 
      Engineering, School of Life Science and Technology, Xi'an Jiaotong University, 
      Xi'an, People's Republic of China (S.-S.X.); Department of Ultrasound, First 
      Affiliated Hospital of Xi'an Jiaotong University, Xi'an, People's Republic of 
      China (H.A.); Shanghai Medical College, Fudan University, Shanghai, People's 
      Republic of China (R.-Y.S.); and Department of Diabetes, Central Clinical School, 
      Monash University, Melbourne, Australia (Z.C., M.E.C.).
FAU - Xu, Shan-Shan
AU  - Xu SS
AD  - Department of Pathology (P.-F.S., T.T., B.W., J.Z., S.-T.R.), Department of 
      Pharmacology (L.-N.C.), and Therapeutic Vaccines Engineering Center of Shaanxi 
      Province (B.W.), School of Basic Medical Sciences, Xi'an Jiaotong University 
      Health Science Center, Xi'an, People's Republic of China; Department of 
      Pathology, Xi'an City Center Hospital, Xi'an, People's Republic of China 
      (P.-F.S.); Department of Nephrology, Second Affiliated Hospital of Xi'an Jiaotong 
      University, Xi'an, People's Republic of China (R.-G.F.); Department of Biomedical 
      Engineering, School of Life Science and Technology, Xi'an Jiaotong University, 
      Xi'an, People's Republic of China (S.-S.X.); Department of Ultrasound, First 
      Affiliated Hospital of Xi'an Jiaotong University, Xi'an, People's Republic of 
      China (H.A.); Shanghai Medical College, Fudan University, Shanghai, People's 
      Republic of China (R.-Y.S.); and Department of Diabetes, Central Clinical School, 
      Monash University, Melbourne, Australia (Z.C., M.E.C.).
FAU - Ai, Hong
AU  - Ai H
AD  - Department of Pathology (P.-F.S., T.T., B.W., J.Z., S.-T.R.), Department of 
      Pharmacology (L.-N.C.), and Therapeutic Vaccines Engineering Center of Shaanxi 
      Province (B.W.), School of Basic Medical Sciences, Xi'an Jiaotong University 
      Health Science Center, Xi'an, People's Republic of China; Department of 
      Pathology, Xi'an City Center Hospital, Xi'an, People's Republic of China 
      (P.-F.S.); Department of Nephrology, Second Affiliated Hospital of Xi'an Jiaotong 
      University, Xi'an, People's Republic of China (R.-G.F.); Department of Biomedical 
      Engineering, School of Life Science and Technology, Xi'an Jiaotong University, 
      Xi'an, People's Republic of China (S.-S.X.); Department of Ultrasound, First 
      Affiliated Hospital of Xi'an Jiaotong University, Xi'an, People's Republic of 
      China (H.A.); Shanghai Medical College, Fudan University, Shanghai, People's 
      Republic of China (R.-Y.S.); and Department of Diabetes, Central Clinical School, 
      Monash University, Melbourne, Australia (Z.C., M.E.C.).
FAU - Wang, Bing
AU  - Wang B
AD  - Department of Pathology (P.-F.S., T.T., B.W., J.Z., S.-T.R.), Department of 
      Pharmacology (L.-N.C.), and Therapeutic Vaccines Engineering Center of Shaanxi 
      Province (B.W.), School of Basic Medical Sciences, Xi'an Jiaotong University 
      Health Science Center, Xi'an, People's Republic of China; Department of 
      Pathology, Xi'an City Center Hospital, Xi'an, People's Republic of China 
      (P.-F.S.); Department of Nephrology, Second Affiliated Hospital of Xi'an Jiaotong 
      University, Xi'an, People's Republic of China (R.-G.F.); Department of Biomedical 
      Engineering, School of Life Science and Technology, Xi'an Jiaotong University, 
      Xi'an, People's Republic of China (S.-S.X.); Department of Ultrasound, First 
      Affiliated Hospital of Xi'an Jiaotong University, Xi'an, People's Republic of 
      China (H.A.); Shanghai Medical College, Fudan University, Shanghai, People's 
      Republic of China (R.-Y.S.); and Department of Diabetes, Central Clinical School, 
      Monash University, Melbourne, Australia (Z.C., M.E.C.).
FAU - Zhang, Jian
AU  - Zhang J
AD  - Department of Pathology (P.-F.S., T.T., B.W., J.Z., S.-T.R.), Department of 
      Pharmacology (L.-N.C.), and Therapeutic Vaccines Engineering Center of Shaanxi 
      Province (B.W.), School of Basic Medical Sciences, Xi'an Jiaotong University 
      Health Science Center, Xi'an, People's Republic of China; Department of 
      Pathology, Xi'an City Center Hospital, Xi'an, People's Republic of China 
      (P.-F.S.); Department of Nephrology, Second Affiliated Hospital of Xi'an Jiaotong 
      University, Xi'an, People's Republic of China (R.-G.F.); Department of Biomedical 
      Engineering, School of Life Science and Technology, Xi'an Jiaotong University, 
      Xi'an, People's Republic of China (S.-S.X.); Department of Ultrasound, First 
      Affiliated Hospital of Xi'an Jiaotong University, Xi'an, People's Republic of 
      China (H.A.); Shanghai Medical College, Fudan University, Shanghai, People's 
      Republic of China (R.-Y.S.); and Department of Diabetes, Central Clinical School, 
      Monash University, Melbourne, Australia (Z.C., M.E.C.).
FAU - Si, Ruo-Yan
AU  - Si RY
AD  - Department of Pathology (P.-F.S., T.T., B.W., J.Z., S.-T.R.), Department of 
      Pharmacology (L.-N.C.), and Therapeutic Vaccines Engineering Center of Shaanxi 
      Province (B.W.), School of Basic Medical Sciences, Xi'an Jiaotong University 
      Health Science Center, Xi'an, People's Republic of China; Department of 
      Pathology, Xi'an City Center Hospital, Xi'an, People's Republic of China 
      (P.-F.S.); Department of Nephrology, Second Affiliated Hospital of Xi'an Jiaotong 
      University, Xi'an, People's Republic of China (R.-G.F.); Department of Biomedical 
      Engineering, School of Life Science and Technology, Xi'an Jiaotong University, 
      Xi'an, People's Republic of China (S.-S.X.); Department of Ultrasound, First 
      Affiliated Hospital of Xi'an Jiaotong University, Xi'an, People's Republic of 
      China (H.A.); Shanghai Medical College, Fudan University, Shanghai, People's 
      Republic of China (R.-Y.S.); and Department of Diabetes, Central Clinical School, 
      Monash University, Melbourne, Australia (Z.C., M.E.C.).
FAU - Chai, Zhonglin
AU  - Chai Z
AD  - Department of Pathology (P.-F.S., T.T., B.W., J.Z., S.-T.R.), Department of 
      Pharmacology (L.-N.C.), and Therapeutic Vaccines Engineering Center of Shaanxi 
      Province (B.W.), School of Basic Medical Sciences, Xi'an Jiaotong University 
      Health Science Center, Xi'an, People's Republic of China; Department of 
      Pathology, Xi'an City Center Hospital, Xi'an, People's Republic of China 
      (P.-F.S.); Department of Nephrology, Second Affiliated Hospital of Xi'an Jiaotong 
      University, Xi'an, People's Republic of China (R.-G.F.); Department of Biomedical 
      Engineering, School of Life Science and Technology, Xi'an Jiaotong University, 
      Xi'an, People's Republic of China (S.-S.X.); Department of Ultrasound, First 
      Affiliated Hospital of Xi'an Jiaotong University, Xi'an, People's Republic of 
      China (H.A.); Shanghai Medical College, Fudan University, Shanghai, People's 
      Republic of China (R.-Y.S.); and Department of Diabetes, Central Clinical School, 
      Monash University, Melbourne, Australia (Z.C., M.E.C.).
FAU - Cooper, Mark E
AU  - Cooper ME
AD  - Department of Pathology (P.-F.S., T.T., B.W., J.Z., S.-T.R.), Department of 
      Pharmacology (L.-N.C.), and Therapeutic Vaccines Engineering Center of Shaanxi 
      Province (B.W.), School of Basic Medical Sciences, Xi'an Jiaotong University 
      Health Science Center, Xi'an, People's Republic of China; Department of 
      Pathology, Xi'an City Center Hospital, Xi'an, People's Republic of China 
      (P.-F.S.); Department of Nephrology, Second Affiliated Hospital of Xi'an Jiaotong 
      University, Xi'an, People's Republic of China (R.-G.F.); Department of Biomedical 
      Engineering, School of Life Science and Technology, Xi'an Jiaotong University, 
      Xi'an, People's Republic of China (S.-S.X.); Department of Ultrasound, First 
      Affiliated Hospital of Xi'an Jiaotong University, Xi'an, People's Republic of 
      China (H.A.); Shanghai Medical College, Fudan University, Shanghai, People's 
      Republic of China (R.-Y.S.); and Department of Diabetes, Central Clinical School, 
      Monash University, Melbourne, Australia (Z.C., M.E.C.).
FAU - Ren, Shu-Ting
AU  - Ren ST
AD  - Department of Pathology (P.-F.S., T.T., B.W., J.Z., S.-T.R.), Department of 
      Pharmacology (L.-N.C.), and Therapeutic Vaccines Engineering Center of Shaanxi 
      Province (B.W.), School of Basic Medical Sciences, Xi'an Jiaotong University 
      Health Science Center, Xi'an, People's Republic of China; Department of 
      Pathology, Xi'an City Center Hospital, Xi'an, People's Republic of China 
      (P.-F.S.); Department of Nephrology, Second Affiliated Hospital of Xi'an Jiaotong 
      University, Xi'an, People's Republic of China (R.-G.F.); Department of Biomedical 
      Engineering, School of Life Science and Technology, Xi'an Jiaotong University, 
      Xi'an, People's Republic of China (S.-S.X.); Department of Ultrasound, First 
      Affiliated Hospital of Xi'an Jiaotong University, Xi'an, People's Republic of 
      China (H.A.); Shanghai Medical College, Fudan University, Shanghai, People's 
      Republic of China (R.-Y.S.); and Department of Diabetes, Central Clinical School, 
      Monash University, Melbourne, Australia (Z.C., M.E.C.) rst@mail.xjtu.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180316
PL  - United States
TA  - J Pharmacol Exp Ther
JT  - The Journal of pharmacology and experimental therapeutics
JID - 0376362
RN  - 0 (Lipopolysaccharides)
RN  - 0 (NF-kappa B)
RN  - 0 (Transforming Growth Factor beta1)
RN  - X4W7ZR7023 (Methylprednisolone)
SB  - IM
MH  - Apoptosis/drug effects
MH  - Cell Line
MH  - Cell Proliferation/drug effects
MH  - Drug Delivery Systems/*methods
MH  - Gene Expression Regulation/drug effects
MH  - Humans
MH  - Intracellular Space/metabolism
MH  - Lipopolysaccharides/*pharmacology
MH  - Mesangial Cells/cytology/*drug effects/metabolism
MH  - Methylprednisolone/metabolism/*pharmacology
MH  - *Microbubbles
MH  - NF-kappa B/metabolism
MH  - Transforming Growth Factor beta1/metabolism
MH  - *Ultrasonic Waves
EDAT- 2018/03/20 06:00
MHDA- 2019/10/15 06:00
CRDT- 2018/03/18 06:00
PHST- 2017/11/07 00:00 [received]
PHST- 2018/03/09 00:00 [accepted]
PHST- 2018/03/20 06:00 [pubmed]
PHST- 2019/10/15 06:00 [medline]
PHST- 2018/03/18 06:00 [entrez]
AID - jpet.117.246223 [pii]
AID - 10.1124/jpet.117.246223 [doi]
PST - ppublish
SO  - J Pharmacol Exp Ther. 2018 Jun;365(3):476-484. doi: 10.1124/jpet.117.246223. Epub 
      2018 Mar 16.