PMID- 29549575
OWN - NLM
STAT- MEDLINE
DCOM- 20190228
LR  - 20190828
IS  - 1860-2002 (Electronic)
IS  - 1536-1632 (Linking)
VI  - 20
IP  - 5
DP  - 2018 Oct
TI  - Apparent Diffusion Coefficient is a Useful Biomarker for Monitoring 
      Adipose-Derived Mesenchymal Stem Cell Therapy of Renal Ischemic-Reperfusion 
      Injury.
PG  - 750-760
LID - 10.1007/s11307-018-1184-0 [doi]
AB  - PURPOSE: This study aimed to investigate the potential of apparent diffusion 
      coefficient (ADC) for monitoring adipose-derived mesenchymal stem cell (ADMSC) 
      therapy of renal ischemic-reperfusion injury (IRI). PROCEDURES: After baseline 
      magnetic resonance imaging (MRI), 36 Sprague-Dawley rats with bilateral renal IRI 
      were divided equally as groups 1, 2, and 3 (non-treated rats) and groups 4, 5, 
      and 6 (ADMSC-treated rats, with 2 million ADMSCs injected via the tail vein at 
      6 h after IRI). Groups 1 and 4, 2 and 5, and 3 and 6 were euthanized at days 1, 
      3, and 7, respectively, after renal MRI. The ratios of ADC at different time 
      points to baseline values in the cortex, outer, and inner stripes of outer 
      medulla (OSOM/ISOM), assessments of monocyte chemoattractant protein-1 (MCP-1), 
      CD68+ cells, tubular cast formation, and degree of fibrosis in three zones over 
      time were compared between the non-treated and ADMSC-treated rats. RESULTS: Among 
      three zones, the differences in cortical ADC and immunohistochemical changes 
      between the non-treated and ADMSC-treated IRI rats over time were less obvious. 
      Compared with the non-treated rats, the ADMSC-treated rats exhibited 
      significantly higher ADC ratios of OSOM and ISOM at days 1 and 3 corresponding to 
      significantly less MCP-1 staining, CD68+ cells, and tubular casts. From day 3 to 
      day 7, coupling with the decrement of MCP-1 and CD68+ cells in IRI kidneys, the 
      effect of cell density on ADC declined. By day 7, the ADMSC-treated rats showed 
      significantly higher ADC ratios of ISOM than the non-treated IRI rats, indicating 
      better recovery, which could be related to significantly fewer tubular casts and 
      marked amelioration of fibrosis. CONCLUSIONS: We suggest ADC is a useful in vivo 
      biomarker for monitoring ADMSC therapy of renal IRI.
FAU - Ko, Sheung-Fat
AU  - Ko SF
AD  - Department of Radiology, Kaohsiung Chang Gung Memorial Hospital and Chang Gung 
      University College of Medicine, 123 Ta-Pei Road, Kaohsiung, Niao-Sung District, 
      833, Taiwan. sfatko@adm.cgmh.org.tw.
FAU - Yip, Hon-Kan
AU  - Yip HK
AD  - Department of Cardiology, Kaohsiung Chang Gung Memorial Hospital and Chang Gung 
      University College of Medicine, Kaohsiung, 833, Taiwan.
AD  - Institute for Translational Research in Biomedicine, Kaohsiung Chang Gung 
      Memorial Hospital and Chang Gung University College of Medicine, Kaohsiung, 833, 
      Taiwan.
FAU - Lee, Chen-Chang
AU  - Lee CC
AD  - Department of Radiology, Kaohsiung Chang Gung Memorial Hospital and Chang Gung 
      University College of Medicine, 123 Ta-Pei Road, Kaohsiung, Niao-Sung District, 
      833, Taiwan.
FAU - Lee, Chia-Chang
AU  - Lee CC
AD  - Department of Medical Research, Kaohsiung Chang Gung Memorial Hospital and Chang 
      Gung University College of Medicine, Kaohsiung, 833, Taiwan.
FAU - Su, Chia-Hao
AU  - Su CH
AD  - Institute for Translational Research in Biomedicine, Kaohsiung Chang Gung 
      Memorial Hospital and Chang Gung University College of Medicine, Kaohsiung, 833, 
      Taiwan.
FAU - Huang, Chung-Cheng
AU  - Huang CC
AD  - Department of Radiology, Kaohsiung Chang Gung Memorial Hospital and Chang Gung 
      University College of Medicine, 123 Ta-Pei Road, Kaohsiung, Niao-Sung District, 
      833, Taiwan.
FAU - Ng, Shu-Hang
AU  - Ng SH
AD  - Department of Radiology, Kaohsiung Chang Gung Memorial Hospital and Chang Gung 
      University College of Medicine, 123 Ta-Pei Road, Kaohsiung, Niao-Sung District, 
      833, Taiwan.
FAU - Chen, Yi-Ling
AU  - Chen YL
AD  - Department of Radiology, Kaohsiung Chang Gung Memorial Hospital and Chang Gung 
      University College of Medicine, 123 Ta-Pei Road, Kaohsiung, Niao-Sung District, 
      833, Taiwan.
FAU - Chen, Min-Chi
AU  - Chen MC
AD  - Department of Biostatistics Consulting Center and Department of Public Health, 
      Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of 
      Medicine, Kaohsiung, 833, Taiwan.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Mol Imaging Biol
JT  - Molecular imaging and biology
JID - 101125610
RN  - 0 (Biomarkers)
RN  - AYI8EX34EU (Creatinine)
SB  - IM
MH  - Adipose Tissue/*cytology
MH  - Animals
MH  - Biomarkers
MH  - Creatinine/blood
MH  - *Diffusion Magnetic Resonance Imaging
MH  - Kidney/diagnostic imaging/*pathology
MH  - Male
MH  - *Mesenchymal Stem Cell Transplantation
MH  - Mesenchymal Stem Cells/*cytology
MH  - Rats, Sprague-Dawley
MH  - Reperfusion Injury/*diagnostic imaging/pathology/*therapy
MH  - Time Factors
OTO - NOTNLM
OT  - Acute renal failure
OT  - Apparent diffusion coefficient
OT  - Diffusion-weighted imaging
OT  - Ischemic-reperfusion injury
OT  - Magnetic resonance imaging
OT  - Stem cells
EDAT- 2018/03/20 06:00
MHDA- 2019/03/01 06:00
CRDT- 2018/03/18 06:00
PHST- 2018/03/20 06:00 [pubmed]
PHST- 2019/03/01 06:00 [medline]
PHST- 2018/03/18 06:00 [entrez]
AID - 10.1007/s11307-018-1184-0 [pii]
AID - 10.1007/s11307-018-1184-0 [doi]
PST - ppublish
SO  - Mol Imaging Biol. 2018 Oct;20(5):750-760. doi: 10.1007/s11307-018-1184-0.