PMID- 29550278
OWN - NLM
STAT- MEDLINE
DCOM- 20181221
LR  - 20221207
IS  - 1096-0007 (Electronic)
IS  - 0014-4835 (Linking)
VI  - 171
DP  - 2018 Jun
TI  - TSLP-activated dendritic cells induce T helper type 2 inflammation in Aspergillus 
      fumigatus keratitis.
PG  - 120-130
LID - S0014-4835(17)30796-0 [pii]
LID - 10.1016/j.exer.2018.03.014 [doi]
AB  - Thymic stromal lymphopoietin (TSLP) is an IL-7-like cytokine, which is secreted 
      by epithelial cells under the stimulation of Toll-like receptor (TLR) ligands. 
      Dendritic cells (DCs) which express the thymic stromal lymphopoietin receptor 
      (TSLPR) can be activated by TSLP. Mature DCs can express the OX40 ligand, which 
      has the ability to combine with OX40 on the surface of T cells to stimulate T 
      cell proliferation. TSLP secreted by corneal epithelial cells can engage in the 
      process of T helper type 2 (Th2) inflammation in Aspergillus fumigatus keratitis, 
      but the mechanism remains unclear. We demonstrated that in A. fumigatus-infected 
      corneas, DCs aggregated, matured, and gradually migrated not only from the 
      basement membrane to the corneal epithelium, but also from the corneal limbus to 
      the central cornea. Mature DCs secreted Th2-attracting chemokines, the thymus and 
      activation-regulated chemokine (TARC), and the macrophage-derived chemokine 
      (MDC), encouraging the secretion of TNF-alpha and Th2 cytokine Interleukin (IL) -4, 
      IL-5, and IL-13. The above processes were all restricted with subconjunctivally 
      injection of TSLP siRNA, while they were strengthened with the injection of 
      rTSLP. We demonstrated that in A. fumigatus keratitis, TSLP, through combination 
      with TSLPR on the surface of DCs, induced DC aggregation, maturation, and 
      migration, and then the mature DCs secreted Th2-attracting chemokines, promoting 
      the secretion of proinflammatory cytokine TNF-alpha and Th2 cytokines, which finally 
      induced Th2 inflammation.
CI  - Copyright (c) 2018 Elsevier Ltd. All rights reserved.
FAU - Sun, Lin
AU  - Sun L
AD  - Department of Ophthalmology, Qilu Hospital of Shandong University, Jinan, 
      Shandong, PR China; The Key Laboratory of Cardiovascular Remodeling and Function 
      Research, Chinese Ministry of Education and Chinese Ministry of Health; The State 
      and Shandong Province Joint Key Laboratory of Translational Cardiovascular 
      Medicine, Qilu Hospital of Shandong University, Jinan, Shandong, PR China.
FAU - Chen, Chen
AU  - Chen C
AD  - Department of Ophthalmology, Qilu Hospital of Shandong University, Jinan, 
      Shandong, PR China; The Key Laboratory of Cardiovascular Remodeling and Function 
      Research, Chinese Ministry of Education and Chinese Ministry of Health; The State 
      and Shandong Province Joint Key Laboratory of Translational Cardiovascular 
      Medicine, Qilu Hospital of Shandong University, Jinan, Shandong, PR China.
FAU - Wu, Jiayin
AU  - Wu J
AD  - Department of Ophthalmology, Qilu Hospital of Shandong University, Jinan, 
      Shandong, PR China.
FAU - Dai, Chenyang
AU  - Dai C
AD  - Department of Ophthalmology, Qilu Hospital of Shandong University, Jinan, 
      Shandong, PR China; The Key Laboratory of Cardiovascular Remodeling and Function 
      Research, Chinese Ministry of Education and Chinese Ministry of Health; The State 
      and Shandong Province Joint Key Laboratory of Translational Cardiovascular 
      Medicine, Qilu Hospital of Shandong University, Jinan, Shandong, PR China.
FAU - Wu, Xinyi
AU  - Wu X
AD  - Department of Ophthalmology, Qilu Hospital of Shandong University, Jinan, 
      Shandong, PR China. Electronic address: xywu8868@163.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180314
PL  - England
TA  - Exp Eye Res
JT  - Experimental eye research
JID - 0370707
RN  - 0 (Chemokines)
RN  - 0 (Cytokines)
RN  - 0 (RNA, Small Interfering)
RN  - GT0IL38SP4 (Thymic Stromal Lymphopoietin)
SB  - IM
MH  - Animals
MH  - Aspergillosis/*immunology/microbiology
MH  - Aspergillus fumigatus
MH  - Blotting, Western
MH  - Cell Movement
MH  - Chemokines/metabolism
MH  - Corneal Ulcer/*immunology/microbiology
MH  - Cytokines/*physiology
MH  - Dendritic Cells/*immunology
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Eye Infections, Fungal/*immunology/microbiology
MH  - Fluorescent Antibody Technique, Indirect
MH  - Inflammation/immunology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - RNA, Small Interfering/genetics
MH  - Real-Time Polymerase Chain Reaction
MH  - Th2 Cells/*immunology
MH  - Thymic Stromal Lymphopoietin
OTO - NOTNLM
OT  - Aspergillus fumigatus
OT  - DC
OT  - TSLP
OT  - Th2 inflammation
EDAT- 2018/03/20 06:00
MHDA- 2018/12/24 06:00
CRDT- 2018/03/19 06:00
PHST- 2017/11/15 00:00 [received]
PHST- 2018/03/08 00:00 [revised]
PHST- 2018/03/13 00:00 [accepted]
PHST- 2018/03/20 06:00 [pubmed]
PHST- 2018/12/24 06:00 [medline]
PHST- 2018/03/19 06:00 [entrez]
AID - S0014-4835(17)30796-0 [pii]
AID - 10.1016/j.exer.2018.03.014 [doi]
PST - ppublish
SO  - Exp Eye Res. 2018 Jun;171:120-130. doi: 10.1016/j.exer.2018.03.014. Epub 2018 Mar 
      14.