PMID- 29551031
OWN - NLM
STAT- MEDLINE
DCOM- 20190221
LR  - 20200717
IS  - 0253-2727 (Print)
IS  - 2707-9740 (Electronic)
IS  - 0253-2727 (Linking)
VI  - 39
IP  - 1
DP  - 2018 Jan 14
TI  - [Brain derived neurotrophic factor enhances the role of mesenchymal stem cells in 
      inhibiting follicular helper T cells].
PG  - 37-40
LID - 10.3760/cma.j.issn.0253-2727.2018.01.008 [doi]
AB  - Objective: To investigate the effect of brain derived neurotrophic factor (BDNF) 
      on mesenchymal stem cells (MSC) inhibiting follicular helper T cells (Tfh cells). 
      Methods: The contents of indoleamine 2,3-dioxygenase (IDO), IL-10, TGF-beta and 
      IL-21 in MSC culture supernatant were detected by ELISA; The peripheral blood of 
      healthy volunteers were collected, and lymphocyte in peripheral blood was 
      separated by human lymphocyte separation solution; Co-cultures of MSC and 
      lymphocyte were performed by Transwell chamber, and the proportion of 
      CD4(+)CXCR5(+) Tfh cells and their subtypes were detected by flow cytometry. 
      Results: 1 in circleThe concentrations of IL-10, TGF-beta, and IDO in the supernatant of BDNF 
      group (BDNF-stimulated MSC) were higher than those of the control ones (adding 
      PBS with the same volume) [IL-10: (42.1+/-4.4) ng/ml vs (19.3+/-2.1) ng/ml, t=4.761, 
      P=0.009; TGF-beta: (13.9+/-1.7) ng/ml vs (5.3+/-0.6) ng/ml, t=5.129, P=0.008; IDO: 
      (441.3+/-56.9) ng/ml vs (226.7+/-37.6) ng/ml, t=3.130, P=0.035]; 2 in circleThe comparisons 
      between BDNF (co-culture of lymphocyte and BDNF-stimulated MSC) and MSC groups 
      (co-culture of lymphocyte and MSC) were detailed as of follows: the proportion of 
      CD4(+) CXCR5(+)Tfh cells were lower [(3.37+/-0.21)% vs (6.51+/-0.27)%, t=9.353, 
      P<0.001], the proportion of CD4(+) CXCR5(+)CXCR3(+) CCR6(-) Tfh cells were higher 
      [(41.14+/-2.04)% vs (26.72+/-2.57)%, t=4.383, P=0.012], CD4(+)CXCR5(+)CXCR3(-)CCR6(-) 
      Tfh2 cells and CD4(+)CXCR5(+)CXCR3(-)CCR6(+) Tfh17 cells were lower [Tfh2: 
      (30.16+/-5.38)% vs (43.26+/-4.11)%, t=4.426, P=0.012; Tfh17: (15.61+/-1.52)% vs 
      (22.32+/-0.72)%, t=4.202, P=0.014], the proportion of CD4(+)CXCR5(+)Foxp3(+) Tfr 
      cells were higher [(4.95+/-0.22)% vs (2.32+/-0.16)%, t=10.241, P<0.001], the 
      concentration of IL-21 in the lymphocyte supernatant was lower [(0.28+/-0.03) ng/ml 
      vs (0.85+/-0.08) ng/ml, t=6.675, P=0.003]. Conclusion: BDNF could enhance the 
      inhibitory effect of MSC on Tfh cells through inhibiting the increasing of Tfh 
      cells and the differentiations of Tfh2 and Tfh17 cells.
FAU - Yang, S N
AU  - Yang SN
AD  - Department of Hematology, Southwest Hospital, Army Medical University, Chongqing 
      400038, China.
FAU - Pu, X
AU  - Pu X
FAU - Xiang, S L
AU  - Xiang SL
FAU - Chen, J P
AU  - Chen JP
FAU - Pei, L
AU  - Pei L
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhonghua Xue Ye Xue Za Zhi
JT  - Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi
JID - 8212398
RN  - 0 (Brain-Derived Neurotrophic Factor)
SB  - IM
MH  - Brain-Derived Neurotrophic Factor
MH  - Cell Differentiation
MH  - Flow Cytometry
MH  - Humans
MH  - *Mesenchymal Stem Cells
MH  - T-Lymphocytes, Helper-Inducer
PMC - PMC7343120
OTO - NOTNLM
OT  - Brain derived neurotrophic factor
OT  - Mesenchymal stem cell
OT  - T-lymphocyte subset
EDAT- 2018/03/20 06:00
MHDA- 2019/02/23 06:00
PMCR- 2018/01/01
CRDT- 2018/03/19 06:00
PHST- 2018/03/19 06:00 [entrez]
PHST- 2018/03/20 06:00 [pubmed]
PHST- 2019/02/23 06:00 [medline]
PHST- 2018/01/01 00:00 [pmc-release]
AID - cjh-39-01-037 [pii]
AID - 10.3760/cma.j.issn.0253-2727.2018.01.008 [doi]
PST - ppublish
SO  - Zhonghua Xue Ye Xue Za Zhi. 2018 Jan 14;39(1):37-40. doi: 
      10.3760/cma.j.issn.0253-2727.2018.01.008.