PMID- 29551464
OWN - NLM
STAT- MEDLINE
DCOM- 20181009
LR  - 20181009
IS  - 1878-3252 (Electronic)
IS  - 0946-672X (Linking)
VI  - 49
DP  - 2018 Sep
TI  - The effects of zinc- and copper-containing welding fumes on murine, rat and human 
      precision-cut lung slices.
PG  - 192-201
LID - S0946-672X(18)30104-4 [pii]
LID - 10.1016/j.jtemb.2018.03.008 [doi]
AB  - Recently, the pro-inflammatory effects of metal inert gas brazing welding fumes 
      containing zinc and copper have been demonstrated in humans. Here, murine, rat 
      and human precision cut lung slices (PCLS) were incubated in welding fume 
      containing media with 0.1, 1, 10 and 100 mug/ml for 24 or 48 h. 24 h incubation 
      were determined either by incubation for the total time or for only 6 h followed 
      by a 18 h post-incubation phase. Cytotoxicity, proliferation and DNA repair 
      rates, and cytokine levels were determined. Welding fume particle concentrations 
      of 0.1 and 1 mug/ml showed no toxic effects on PCLS of all three species, while 
      for 10 and 100 mug/ml a concentration-dependent toxicity occurred. Proliferation 
      and DNA repair rates were reduced for all tested concentrations and incubation 
      times. Additionally, the cytokine levels in the supernatants were markedly 
      reduced, while after 6 h of exposure with 18 h of post-incubation time a trend 
      towards increased cytokine levels occurred. PCLS are a reliable and feasible 
      method to assess and offer a prediction of toxic effects of welding fume 
      particles on human lungs. Rat PCLS showed similar responses compared to human 
      PCLS and are suitable for further evaluation of toxic effects exerted by welding 
      fume particles.
CI  - Copyright (c) 2018 Elsevier GmbH. All rights reserved.
FAU - Krabbe, Julia
AU  - Krabbe J
AD  - Institute of Pharmacology and Toxicology, Medical Faculty, RWTH Aachen 
      University, Wendlingweg 2, 52074 Aachen, Germany; Institute of Occupational, 
      Social and Environmental Medicine, Medical Faculty, RWTH Aachen University, 
      Pauwelsstrasse 30, 52074 Aachen, Germany. Electronic address: jkrabbe@ukaachen.de.
FAU - Esser, Andre
AU  - Esser A
AD  - Institute of Occupational, Social and Environmental Medicine, Medical Faculty, 
      RWTH Aachen University, Pauwelsstrasse 30, 52074 Aachen, Germany.
FAU - Kanzler, Stephanie
AU  - Kanzler S
AD  - Institute of Pharmacology and Toxicology, Medical Faculty, RWTH Aachen 
      University, Wendlingweg 2, 52074 Aachen, Germany.
FAU - Braunschweig, Till
AU  - Braunschweig T
AD  - Institute of Pathology, Medical Faculty, RWTH Aachen University, Pauwelsstrasse 
      30, 52074 Aachen, Germany.
FAU - Kintsler, Svetlana
AU  - Kintsler S
AD  - Institute of Pathology, Medical Faculty, RWTH Aachen University, Pauwelsstrasse 
      30, 52074 Aachen, Germany.
FAU - Spillner, Jan
AU  - Spillner J
AD  - Departement of Thoracic and Cardiovascular Surgery, Medical Faculty, RWTH Aachen 
      University, Pauwelsstrasse 30, 52074 Aachen, Germany.
FAU - Schroder, Thomas
AU  - Schroder T
AD  - Department of Surgery, Luisenhospital Aachen, Boxgraben 99, 52064 Aachen, 
      Germany.
FAU - Kalverkamp, Sebastian
AU  - Kalverkamp S
AD  - Departement of Thoracic and Cardiovascular Surgery, Medical Faculty, RWTH Aachen 
      University, Pauwelsstrasse 30, 52074 Aachen, Germany.
FAU - Balakirski, Galina
AU  - Balakirski G
AD  - Institute of Pharmacology and Toxicology, Medical Faculty, RWTH Aachen 
      University, Wendlingweg 2, 52074 Aachen, Germany; Departement of Dermatology and 
      Allergology, Medical Faculty, RWTH Aachen University, Pauwelsstrasse 30, 52074 
      Aachen, Germany.
FAU - Gerhards, Benjamin
AU  - Gerhards B
AD  - ISF- Welding and Joining Institute, RWTH Aachen University, Pontstrasse 49, 52062 
      Aachen, Germany.
FAU - Rieg, Annette D
AU  - Rieg AD
AD  - Department of Anaesthesiology, Medical Faculty, RWTH Aachen University, 
      Pauwelsstrasse 30, 52074 Aachen, Germany.
FAU - Kraus, Thomas
AU  - Kraus T
AD  - Institute of Occupational, Social and Environmental Medicine, Medical Faculty, 
      RWTH Aachen University, Pauwelsstrasse 30, 52074 Aachen, Germany.
FAU - Brand, Peter
AU  - Brand P
AD  - Institute of Occupational, Social and Environmental Medicine, Medical Faculty, 
      RWTH Aachen University, Pauwelsstrasse 30, 52074 Aachen, Germany.
FAU - Martin, Christian
AU  - Martin C
AD  - Institute of Pharmacology and Toxicology, Medical Faculty, RWTH Aachen 
      University, Wendlingweg 2, 52074 Aachen, Germany.
LA  - eng
PT  - Journal Article
DEP - 20180310
PL  - Germany
TA  - J Trace Elem Med Biol
JT  - Journal of trace elements in medicine and biology : organ of the Society for 
      Minerals and Trace Elements (GMS)
JID - 9508274
RN  - 0 (Air Pollutants, Occupational)
RN  - 789U1901C5 (Copper)
RN  - J41CSQ7QDS (Zinc)
SB  - IM
MH  - Air Pollutants, Occupational/*toxicity
MH  - Animals
MH  - Copper/*chemistry
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Humans
MH  - In Vitro Techniques
MH  - Inhalation Exposure/adverse effects
MH  - Lung/*drug effects
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Rats
MH  - *Rats, Wistar
MH  - Welding
MH  - Zinc/*chemistry
OTO - NOTNLM
OT  - Copper-containing metal fumes
OT  - Metal inert gas brazing
OT  - Precision-cut lung slices
OT  - Toxicology
OT  - Welding fumes
OT  - Zinc-containing metal fumes
EDAT- 2018/03/20 06:00
MHDA- 2018/10/10 06:00
CRDT- 2018/03/20 06:00
PHST- 2018/01/30 00:00 [received]
PHST- 2018/03/07 00:00 [revised]
PHST- 2018/03/08 00:00 [accepted]
PHST- 2018/03/20 06:00 [pubmed]
PHST- 2018/10/10 06:00 [medline]
PHST- 2018/03/20 06:00 [entrez]
AID - S0946-672X(18)30104-4 [pii]
AID - 10.1016/j.jtemb.2018.03.008 [doi]
PST - ppublish
SO  - J Trace Elem Med Biol. 2018 Sep;49:192-201. doi: 10.1016/j.jtemb.2018.03.008. 
      Epub 2018 Mar 10.