PMID- 29552054
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 1735-0328 (Print)
IS  - 1726-6890 (Electronic)
IS  - 1726-6882 (Linking)
VI  - 16
IP  - 4
DP  - 2017 Fall
TI  - Study of the Role of CREB, BDNF, and VGF Neuropeptide in Long Term Antidepressant 
      Activity of Crocin in the Rat Cerebellum.
PG  - 1452-1462
AB  - Antidepressant activity of crocin, saffron main component, has been established 
      before. Based on previous study, it is suggested that elevation in the levels of 
      BDNF (brain-derived neurotrophic factor), CREB (cAMP response element binding) 
      and VGF neuropeptide could be considered as one probable molecular mechanisms 
      involved in antidepressant activity of long term crocin administration in the rat 
      hippocampus. In this study we further investigated whether the antidepressant 
      activity of crocin in long term administration was associated with alteration in 
      these factors in the rat cerebellum. Crocin (12.5, 25 and 50 mg/kg/day) and 
      imipramine (10 mg/kg/day) were administered interaperitoneally for 21 days to 
      rats. At the end of experiment, animals were sacrificed and cerebellums were 
      dissected. BDNF, VGF, CREB, and phospho-CREB (P-CREB) protein and mRNA levels in 
      the rat cerebellum were evaluated using Western blot and quantitative reverse 
      transcription-polymerase chain reaction (qRT-PCR). In the current study 
      significant increases in mRNA and protein levels of VGF, CREB and (BDNF) in long 
      term crocin treatment were not observed in the rat cerebellum. Although a slight 
      increase was observed in protein level of P-CREB compared to normal saline, but 
      it was not significant. It is concluded that antidepressant activity of crocin 
      might be partially mediated to CREB. Moreover, other factors rather than BDNF and 
      VGF neuropeptides may alter following long term crocin treatment in the 
      cerebellum. To understand the precise mechanism of crocin antidepressant effects 
      in the cerebellum, longer duration of crocin treatment in further studies is 
      recommended.
FAU - Razavi, Bibi Marjan
AU  - Razavi BM
AD  - Targeted Drug Delivery Research Center, Mashhad University of Medical Sciences, 
      Mashhad, Iran.
AD  - Department of Pharmacodynamy and Toxicology, School of Pharmacy, Mashhad 
      University of Medical Sciences, Mashhad, Iran.
FAU - Sadeghi, Mahdieh
AU  - Sadeghi M
AD  - Department of Pharmacodynamy and Toxicology, School of Pharmacy, Mashhad 
      University of Medical Sciences, Mashhad, Iran.
FAU - Abnous, Khalil
AU  - Abnous K
AD  - Pharmaceutical Research Center, Pharmaceutical Technology Institute, Mashhad 
      University of Medical Sciences, Mashhad, Iran.
AD  - Department of Medicinal Chemistry, School of Pharmacy, Mashhad University of 
      Medical Sciences, Mashhad, Iran.
FAU - Vahdati Hasani, Faezeh
AU  - Vahdati Hasani F
AD  - Department of Pharmacodynamy and Toxicology, School of Pharmacy, Mashhad 
      University of Medical Sciences, Mashhad, Iran.
FAU - Hosseinzadeh, Hossein
AU  - Hosseinzadeh H
AD  - Pharmaceutical Research Center, Pharmaceutical Technology Institute, Mashhad 
      University of Medical Sciences, Mashhad, Iran.
AD  - Department of Pharmacodynamy and Toxicology, School of Pharmacy, Mashhad 
      University of Medical Sciences, Mashhad, Iran.
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Iran J Pharm Res
JT  - Iranian journal of pharmaceutical research : IJPR
JID - 101208407
PMC - PMC5843307
OTO - NOTNLM
OT  - BDNF
OT  - CREB
OT  - Cerebellum
OT  - Crocin
OT  - Depression
OT  - P-CREB
OT  - Saffron
EDAT- 2018/03/20 06:00
MHDA- 2018/03/20 06:01
PMCR- 2017/09/01
CRDT- 2018/03/20 06:00
PHST- 2018/03/20 06:00 [entrez]
PHST- 2018/03/20 06:00 [pubmed]
PHST- 2018/03/20 06:01 [medline]
PHST- 2017/09/01 00:00 [pmc-release]
AID - IJPR-16-1452 [pii]
PST - ppublish
SO  - Iran J Pharm Res. 2017 Fall;16(4):1452-1462.