PMID- 29553543
OWN - NLM
STAT- MEDLINE
DCOM- 20180621
LR  - 20200212
IS  - 1940-087X (Electronic)
IS  - 1940-087X (Linking)
IP  - 132
DP  - 2018 Feb 12
TI  - Regenerative Therapy by Suprachoroidal Cell Autograft in Dry Age-related Macular 
      Degeneration: Preliminary In Vivo Report.
LID - 10.3791/56469 [doi]
LID - 56469
AB  - This study is aimed at examining whether a suprachoroidal graft of autologous 
      cells can improve best corrected visual acuity (BCVA) and responses to 
      microperimetry (MY) in eyes affected by dry Age-related Macular Degeneration 
      (AMD) over time through the production and secretion of growth factors (GFs) on 
      surrounding tissue. Patients were randomly assigned to each study group. All 
      patients were diagnosed with dry AMD and with BCVA equal to or greater than 1 
      logarithm of the minimum angle of resolution (logMAR). A suprachoroidal 
      autologous graft by Limoli Retinal Restoration Technique (LRRT) was carried out 
      on group A, which included 11 eyes from 11 patients. The technique was performed 
      by implanting adipocytes, adipose-derived stem cells obtained from the stromal 
      vascular fraction, and platelets from platelet-rich plasma in the suprachoroidal 
      space. Conversely, group B, including 14 eyes of 14 patients, was used as a 
      control group. For each patient, diagnosis was verified by confocal scanning 
      laser ophthalmoscope and spectral domain-optical coherence tomography (SD-OCT). 
      In group A, BCVA improved by 0.581 to 0.504 at 90 days and to 0.376 logMAR at 180 
      days (+32.20%) postoperatively. Furthermore, MY test increased by 11.44 dB to 
      12.59 dB at 180 days. The different cell types grafted behind the choroid were 
      able to ensure constant GF secretion in the choroidal flow. Consequently, the 
      results indicate that visual acuity (VA) in the grafted group can increase more 
      than in the control group after six months.
FAU - Limoli, Paolo Giuseppe
AU  - Limoli PG
AD  - Low Vision Research Centre of Milan.
FAU - Vingolo, Enzo Maria
AU  - Vingolo EM
AD  - Department of Ophthalmology, A. Fiorini Hospital, Sapienza University of Rome.
FAU - Limoli, Celeste
AU  - Limoli C
AD  - Low Vision Research Centre of Milan.
FAU - Scalinci, Sergio Zaccaria
AU  - Scalinci SZ
AD  - Glaucoma and Low Vision Study Center, Department of General Surgery and Organ 
      Transplants, University of Bologna.
FAU - Nebbioso, Marcella
AU  - Nebbioso M
AD  - Department of Sense Organs, Faculty of Medicine and Odontology, Sapienza 
      University of Rome; marcella.nebbioso@uniroma1.it.
LA  - eng
PT  - Journal Article
PT  - Randomized Controlled Trial
PT  - Video-Audio Media
DEP - 20180212
PL  - United States
TA  - J Vis Exp
JT  - Journal of visualized experiments : JoVE
JID - 101313252
SB  - IM
MH  - Adipose Tissue/cytology/*transplantation
MH  - Aged
MH  - Aged, 80 and over
MH  - Choroid/surgery
MH  - Female
MH  - Humans
MH  - Macular Degeneration/surgery/*therapy
MH  - Male
MH  - Middle Aged
MH  - Stem Cell Transplantation/*methods
MH  - Transplantation, Autologous
PMC - PMC5912396
EDAT- 2018/03/20 06:00
MHDA- 2018/06/22 06:00
PMCR- 2020/02/12
CRDT- 2018/03/20 06:00
PHST- 2018/03/20 06:00 [entrez]
PHST- 2018/03/20 06:00 [pubmed]
PHST- 2018/06/22 06:00 [medline]
PHST- 2020/02/12 00:00 [pmc-release]
AID - 56469 [pii]
AID - 10.3791/56469 [doi]
PST - epublish
SO  - J Vis Exp. 2018 Feb 12;(132):56469. doi: 10.3791/56469.