PMID- 29553857
OWN - NLM
STAT- MEDLINE
DCOM- 20190417
LR  - 20190417
IS  - 1369-1635 (Electronic)
IS  - 0953-7104 (Linking)
VI  - 30
IP  - 3
DP  - 2019
TI  - A comprehensive proteomics study on platelet concentrates: Platelet proteome, 
      storage time and Mirasol pathogen reduction technology.
PG  - 368-379
LID - 10.1080/09537104.2018.1447658 [doi]
AB  - Platelet concentrates (PCs) represent a blood transfusion product with a major 
      concern for safety as their storage temperature (20-24 degrees C) allows bacterial 
      growth, and their maximum storage time period (less than a week) precludes 
      complete microbiological testing. Pathogen inactivation technologies (PITs) 
      provide an additional layer of safety to the blood transfusion products from 
      known and unknown pathogens such as bacteria, viruses, and parasites. In this 
      context, PITs, such as Mirasol Pathogen Reduction Technology (PRT), have been 
      developed and are implemented in many countries. However, several studies have 
      shown in vitro that Mirasol PRT induces a certain level of platelet shape change, 
      hyperactivation, basal degranulation, and increased oxidative damage during 
      storage. It has been suggested that Mirasol PRT might accelerate what has been 
      described as the platelet storage lesion (PSL), but supportive molecular 
      signatures have not been obtained. We aimed at dissecting the influence of both 
      variables, that is, Mirasol PRT and storage time, at the proteome level. We 
      present comprehensive proteomics data analysis of Control PCs and PCs treated 
      with Mirasol PRT at storage days 1, 2, 6, and 8. Our workflow was set to perform 
      proteomics analysis using a gel-free and label-free quantification (LFQ) 
      approach. Semi-quantification was based on LFQ signal intensities of identified 
      proteins using MaxQuant/Perseus software platform. Data are available via 
      ProteomeXchange with identifier PXD008119. We identified marginal differences 
      between Mirasol PRT and Control PCs during storage. However, those significant 
      changes at the proteome level were specifically related to the functional aspects 
      previously described to affect platelets upon Mirasol PRT. In addition, the 
      effect of Mirasol PRT on the platelet proteome appeared not to be exclusively due 
      to an accelerated or enhanced PSL. In summary, semi-quantitative proteomics 
      allows to discern between proteome changes due to Mirasol PRT or PSL, and proves 
      to be a methodology suitable to phenotype platelets in an unbiased manner, in 
      various physiological contexts.
FAU - Salunkhe, Vishal
AU  - Salunkhe V
AD  - a Department of Blood Cell Research , Sanquin Research and Landsteiner 
      Laboratory, Academic Medical Centre (AMC), University of Amsterdam (UvA) , 
      Amsterdam , The Netherlands.
FAU - De Cuyper, Iris M
AU  - De Cuyper IM
AUID- ORCID: 0000-0001-8862-8000
AD  - a Department of Blood Cell Research , Sanquin Research and Landsteiner 
      Laboratory, Academic Medical Centre (AMC), University of Amsterdam (UvA) , 
      Amsterdam , The Netherlands.
FAU - Papadopoulos, Petros
AU  - Papadopoulos P
AUID- ORCID: 0000-0002-8342-329X
AD  - b Department of Hematology , Hospital Clinico San Carlos, Instituto de 
      Investigacion Sanitaria San Carlos (IdISSC) , Madrid , Spain.
FAU - van der Meer, Pieter F
AU  - van der Meer PF
AUID- ORCID: 0000-0002-2093-3604
AD  - c Department of Product and Process Development , Sanquin Blood Bank , Amsterdam 
      , The Netherlands.
FAU - Daal, Brunette B
AU  - Daal BB
AD  - c Department of Product and Process Development , Sanquin Blood Bank , Amsterdam 
      , The Netherlands.
FAU - Villa-Fajardo, Maria
AU  - Villa-Fajardo M
AD  - b Department of Hematology , Hospital Clinico San Carlos, Instituto de 
      Investigacion Sanitaria San Carlos (IdISSC) , Madrid , Spain.
FAU - de Korte, Dirk
AU  - de Korte D
AUID- ORCID: 0000-0001-9772-7377
AD  - a Department of Blood Cell Research , Sanquin Research and Landsteiner 
      Laboratory, Academic Medical Centre (AMC), University of Amsterdam (UvA) , 
      Amsterdam , The Netherlands.
AD  - c Department of Product and Process Development , Sanquin Blood Bank , Amsterdam 
      , The Netherlands.
FAU - van den Berg, Timo K
AU  - van den Berg TK
AD  - a Department of Blood Cell Research , Sanquin Research and Landsteiner 
      Laboratory, Academic Medical Centre (AMC), University of Amsterdam (UvA) , 
      Amsterdam , The Netherlands.
FAU - Gutierrez, Laura
AU  - Gutierrez L
AUID- ORCID: 0000-0001-8443-900X
AD  - a Department of Blood Cell Research , Sanquin Research and Landsteiner 
      Laboratory, Academic Medical Centre (AMC), University of Amsterdam (UvA) , 
      Amsterdam , The Netherlands.
AD  - b Department of Hematology , Hospital Clinico San Carlos, Instituto de 
      Investigacion Sanitaria San Carlos (IdISSC) , Madrid , Spain.
LA  - eng
PT  - Journal Article
DEP - 20180319
PL  - England
TA  - Platelets
JT  - Platelets
JID - 9208117
RN  - 0 (Proteome)
SB  - IM
MH  - Blood Platelets/*metabolism
MH  - Humans
MH  - Platelet Function Tests/*methods
MH  - Proteome/*metabolism
MH  - Proteomics/*methods
OTO - NOTNLM
OT  - Blood safety
OT  - pathogen inactivation technologies
OT  - platelet function tests
OT  - platelet transfusion
OT  - proteomics
OT  - storage time
EDAT- 2018/03/20 06:00
MHDA- 2019/04/18 06:00
CRDT- 2018/03/20 06:00
PHST- 2018/03/20 06:00 [pubmed]
PHST- 2019/04/18 06:00 [medline]
PHST- 2018/03/20 06:00 [entrez]
AID - 10.1080/09537104.2018.1447658 [doi]
PST - ppublish
SO  - Platelets. 2019;30(3):368-379. doi: 10.1080/09537104.2018.1447658. Epub 2018 Mar 
      19.