PMID- 29555351
OWN - NLM
STAT- MEDLINE
DCOM- 20190305
LR  - 20190305
IS  - 1534-4436 (Electronic)
IS  - 1081-1206 (Linking)
VI  - 121
IP  - 1
DP  - 2018 Jul
TI  - Mycoplasma pneumoniae induces allergy by producing P1-specific immunoglobulin E.
PG  - 90-97
LID - S1081-1206(18)30212-6 [pii]
LID - 10.1016/j.anai.2018.03.014 [doi]
AB  - BACKGROUND: Our previous study found that most Mycoplasma pneumoniae (MP) 
      pneumonia (MPP)patients had elevated serum total immunoglobulin E (IgE) levels. 
      OBJECTIVE: To determine components of MP that can cause an IgE increase in 
      children, and to clarify its specific mechanism. METHODS: The components of MP 
      cells were isolated by serum IgE from patients with MP pneumonia. These 
      components obtained through the prokaryotic expression were used as allergens to 
      detect the proportion of allergen-specific IgE produced in MPP patients, and the 
      clinical characteristics and related immune parameters of these patients who 
      produced this allergen-specific IgE were also analyzed. In addition, a cell 
      experiment was used to verify the biological effect of these components in vitro. 
      RESULTS: P1-specific IgE was detected in serum of MPP children. An approximately 
      24-kDa polypeptide of P1 protein was obtained through prokaryotic expression 
      purified by nickel agarose affinity chromatography. Approximately 9.2% of MPP 
      patients produced IgE against this polypeptide of P1 protein, which was more 
      likely to be produced in MPP patients with no history of allergies or family 
      history of allergy-related diseases. P1-specific IgE-positive MPP patients had 
      more severe clinical symptoms, with excessive secretion of interleukin (IL)-4 and 
      IL-5 and overdifferentiation of Th0 cells into Th2 cells. Tests also demonstrated 
      that the P1 protein stimulated excessive secretion of IL-4 and IL-5 in peripheral 
      blood mononuclear cells from the peripheral blood of healthy donors. CONCLUSION: 
      Mycoplasma pneumoniae is not only an infectious agent but also an allergen for 
      certain individuals. The P1 protein of MP can induce the production of 
      P1-specific IgE.
CI  - Copyright (c) 2018 American College of Allergy, Asthma & Immunology. Published by 
      Elsevier Inc. All rights reserved.
FAU - Ye, Qing
AU  - Ye Q
AD  - Zhejiang Key Laboratory for Neonatal Diseases, The Children's Hospital of 
      Zhejiang University School of Medicine, Hangzhou, China. Electronic address: 
      qingye@zju.edu.
FAU - Mao, Jian-Hua
AU  - Mao JH
AD  - Zhejiang Key Laboratory for Neonatal Diseases, The Children's Hospital of 
      Zhejiang University School of Medicine, Hangzhou, China.
FAU - Shu, Qiang
AU  - Shu Q
AD  - Zhejiang Key Laboratory for Neonatal Diseases, The Children's Hospital of 
      Zhejiang University School of Medicine, Hangzhou, China.
FAU - Shang, Shi-Qiang
AU  - Shang SQ
AD  - Zhejiang Key Laboratory for Neonatal Diseases, The Children's Hospital of 
      Zhejiang University School of Medicine, Hangzhou, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180316
PL  - United States
TA  - Ann Allergy Asthma Immunol
JT  - Annals of allergy, asthma & immunology : official publication of the American 
      College of Allergy, Asthma, & Immunology
JID - 9503580
RN  - 0 (Allergens)
RN  - 0 (Bacterial Proteins)
RN  - 0 (IL4 protein, human)
RN  - 0 (IL5 protein, human)
RN  - 0 (Interleukin-5)
RN  - 0 (Recombinant Proteins)
RN  - 207137-56-2 (Interleukin-4)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Allergens/genetics/*immunology
MH  - Bacterial Proteins/genetics/*immunology
MH  - Child
MH  - Child, Preschool
MH  - Cloning, Molecular
MH  - Escherichia coli/genetics/metabolism
MH  - Female
MH  - Gene Expression
MH  - Genetic Vectors/chemistry/metabolism
MH  - Humans
MH  - Immunoglobulin E/*biosynthesis/blood
MH  - Infant
MH  - Interleukin-4/genetics/immunology
MH  - Interleukin-5/genetics/immunology
MH  - Male
MH  - Mycoplasma pneumoniae/chemistry/*immunology/pathogenicity
MH  - Pneumonia, Mycoplasma/*immunology/microbiology/pathology
MH  - Prospective Studies
MH  - Recombinant Proteins/genetics/immunology
MH  - Th1 Cells/immunology/microbiology
MH  - Th2 Cells/immunology/microbiology
EDAT- 2018/03/21 06:00
MHDA- 2019/03/06 06:00
CRDT- 2018/03/21 06:00
PHST- 2017/12/20 00:00 [received]
PHST- 2018/03/01 00:00 [revised]
PHST- 2018/03/12 00:00 [accepted]
PHST- 2018/03/21 06:00 [pubmed]
PHST- 2019/03/06 06:00 [medline]
PHST- 2018/03/21 06:00 [entrez]
AID - S1081-1206(18)30212-6 [pii]
AID - 10.1016/j.anai.2018.03.014 [doi]
PST - ppublish
SO  - Ann Allergy Asthma Immunol. 2018 Jul;121(1):90-97. doi: 
      10.1016/j.anai.2018.03.014. Epub 2018 Mar 16.