PMID- 29558733
OWN - NLM
STAT- MEDLINE
DCOM- 20180917
LR  - 20180917
IS  - 1873-4235 (Electronic)
IS  - 0956-5663 (Linking)
VI  - 109
DP  - 2018 Jun 30
TI  - Ultrasensitive photoelectrochemical biosensor for the detection of HTLV-I DNA: A 
      cascade signal amplification strategy integrating lambda-exonuclease aided target 
      recycling with hybridization chain reaction and enzyme catalysis.
PG  - 190-196
LID - S0956-5663(18)30196-9 [pii]
LID - 10.1016/j.bios.2018.03.023 [doi]
AB  - Sensitive and specific detection of DNA is of great significance for clinical 
      diagnosis. In this paper, an effective cascade signal amplification strategy was 
      introduced into photoelectrochemical (PEC) biosensor for ultrasensitive detection 
      of human T-cell lymphotropic virus type I (HTLV-I) DNA. This proposed signal 
      amplification strategy integrates lambda-exonuclease (lambda-Exo) aided target recycling 
      with hybridization chain reaction (HCR) and enzyme catalysis. In the presence of 
      target DNA (tDNA) of HTLV-I, the designed hairpin DNA (h(1)DNA) hybridized with 
      tDNA, subsequently recognized and cleaved by lambda-Exo to set free tDNA. With the 
      lambda-Exo aided tDNA recycling, an increasing number of DNA fragments (output DNA, 
      oDNA) were released from the digestion of h(1)DNA. Then, triggered by the 
      hybridization of oDNA with capture DNA (cDNA), numerous biotin-labeled hairpin 
      DNAs (h(2)DNA and h(3)DNA) could be loaded onto the photoelectrode via the HCR. 
      Finally, avidin-labeled alkaline phosphatase (avidin-ALP) could be introduced 
      onto the electrode by specific interaction between biotin and avidin. The ALP 
      could catalyze dephosphorylation of phospho-L-ascorbic acid trisodium salt (AAP) 
      to generate an efficient electron donor of ascorbic acid (AA), and thereby 
      greatly increasing the photocurrent signal. By utilizing the proposed cascade 
      signal amplification strategy, the fabricated PEC biosensor exhibited an 
      ultrasensitive and specific detection of HTLV-I DNA down to 11.3 aM, and it also 
      offered an effective strategy to detect other DNAs at ultralow levels.
CI  - Copyright (c) 2018 Elsevier B.V. All rights reserved.
FAU - Shi, Xiao-Mei
AU  - Shi XM
AD  - Key Laboratory for Organic Electronics and Information Displays & Jiangsu Key 
      Laboratory for Biosensors, Institute of Advanced Materials (IAM), Jiangsu 
      National Synergetic Innovation Center for Advanced Materials (SICAM), Nanjing 
      University of Posts & Telecommunications, Nanjing 210023, PR China; State Key 
      Laboratory of Analytical Chemistry for Life Science, School of Chemistry and 
      Chemical Engineering, Nanjing University, Nanjing 210093, PR China.
FAU - Fan, Gao-Chao
AU  - Fan GC
AD  - Key Laboratory of Sensor Analysis of Tumor Marker of Education Ministry, College 
      of Chemistry and Molecular Engineering, Qingdao University of Science and 
      Technology, Qingdao 266042, PR China; State Key Laboratory of Analytical 
      Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing 
      University, Nanjing 210093, PR China.
FAU - Tang, Xueying
AU  - Tang X
AD  - Key Laboratory for Organic Electronics and Information Displays & Jiangsu Key 
      Laboratory for Biosensors, Institute of Advanced Materials (IAM), Jiangsu 
      National Synergetic Innovation Center for Advanced Materials (SICAM), Nanjing 
      University of Posts & Telecommunications, Nanjing 210023, PR China.
FAU - Shen, Qingming
AU  - Shen Q
AD  - Key Laboratory for Organic Electronics and Information Displays & Jiangsu Key 
      Laboratory for Biosensors, Institute of Advanced Materials (IAM), Jiangsu 
      National Synergetic Innovation Center for Advanced Materials (SICAM), Nanjing 
      University of Posts & Telecommunications, Nanjing 210023, PR China. Electronic 
      address: iamqmshen@njupt.edu.cn.
FAU - Zhu, Jun-Jie
AU  - Zhu JJ
AD  - State Key Laboratory of Analytical Chemistry for Life Science, School of 
      Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, PR China. 
      Electronic address: jjzhu@nju.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20180313
PL  - England
TA  - Biosens Bioelectron
JT  - Biosensors & bioelectronics
JID - 9001289
RN  - 0 (DNA, Viral)
RN  - EC 3.1.- (Exodeoxyribonucleases)
RN  - EC 3.1.3.1 (Alkaline Phosphatase)
SB  - IM
MH  - Alkaline Phosphatase/chemistry
MH  - *Biosensing Techniques
MH  - *Catalysis
MH  - Conductometry
MH  - DNA, Viral/chemistry/genetics/*isolation & purification
MH  - Exodeoxyribonucleases/chemistry/genetics
MH  - Human T-lymphotropic virus 1/chemistry/genetics/*isolation & purification
MH  - Humans
MH  - Nucleic Acid Hybridization/genetics
OTO - NOTNLM
OT  - DNA sensor
OT  - HTLV-I
OT  - Hybridization chain reaction
OT  - Photoelectrochemistry
OT  - Signal amplification
EDAT- 2018/03/21 06:00
MHDA- 2018/09/18 06:00
CRDT- 2018/03/21 06:00
PHST- 2018/02/03 00:00 [received]
PHST- 2018/03/09 00:00 [revised]
PHST- 2018/03/12 00:00 [accepted]
PHST- 2018/03/21 06:00 [pubmed]
PHST- 2018/09/18 06:00 [medline]
PHST- 2018/03/21 06:00 [entrez]
AID - S0956-5663(18)30196-9 [pii]
AID - 10.1016/j.bios.2018.03.023 [doi]
PST - ppublish
SO  - Biosens Bioelectron. 2018 Jun 30;109:190-196. doi: 10.1016/j.bios.2018.03.023. 
      Epub 2018 Mar 13.