PMID- 29561214
OWN - NLM
STAT- MEDLINE
DCOM- 20200526
LR  - 20200901
IS  - 1559-7016 (Electronic)
IS  - 0271-678X (Print)
IS  - 0271-678X (Linking)
VI  - 39
IP  - 9
DP  - 2019 Sep
TI  - Glucose transporter 2 mediates the hypoglycemia-induced increase in cerebral 
      blood flow.
PG  - 1725-1736
LID - 10.1177/0271678X18766743 [doi]
AB  - Glucose transporter 2 (Glut2)-positive cells are sparsely distributed in brain 
      and play an important role in the stimulation of glucagon secretion in response 
      to hypoglycemia. We aimed to determine if Glut2-positive cells can influence 
      another response to hypoglycemia, i.e. increased cerebral blood flow (CBF). CBF 
      of adult male mice devoid of Glut2, either globally (ripglut1:glut2(-)(/)(-)) or 
      in the nervous system only (NG2KO), and their respective controls were studied 
      under basal glycemia and insulin-induced hypoglycemia using quantitative 
      perfusion magnetic resonance imaging at 9.4 T. The effect on CBF of optogenetic 
      activation of hypoglycemia responsive Glut2-positive neurons of the 
      paraventricular thalamic area was measured in mice expressing channelrhodopsin2 
      under the control of the Glut2 promoter. We found that in both 
      ripglut1:glut2(-)(/)(-) mice and NG2KO mice, CBF in basal conditions was higher 
      than in their respective controls and not further activated by hypoglycemia, as 
      measured in the hippocampus, hypothalamus and whole brain. Conversely, 
      optogenetic activation of Glut2-positive cells in the paraventricular thalamic 
      nucleus induced a local increase in CBF similar to that induced by hypoglycemia. 
      Thus, Glut2 expression in the nervous system is required for the control of CBF 
      in response to changes in blood glucose concentrations.
FAU - Lei, Hongxia
AU  - Lei H
AUID- ORCID: 0000-0002-4065-9331
AD  - 1 AIT, Center for Biomedical Imaging (CIBM-AIT), Ecole Polytechnique Federale de 
      Lausanne, Lausanne, Switzerland.
AD  - 2 Department of Radiology, University of Geneva, Geneva, Switzerland.
FAU - Preitner, Frederic
AU  - Preitner F
AD  - 3 Center for Integrative Genomics, University of Lausanne, Lausanne, Switzerland.
AD  - 4 Mouse Metabolic Evaluation Facility, Center for Integrative Genomics, 
      University of Lausanne, Lausanne, Switzerland.
FAU - Labouebe, Gwenael
AU  - Labouebe G
AD  - 3 Center for Integrative Genomics, University of Lausanne, Lausanne, Switzerland.
FAU - Gruetter, Rolf
AU  - Gruetter R
AD  - 1 AIT, Center for Biomedical Imaging (CIBM-AIT), Ecole Polytechnique Federale de 
      Lausanne, Lausanne, Switzerland.
AD  - 2 Department of Radiology, University of Geneva, Geneva, Switzerland.
AD  - 5 Department of Radiology, University of Lausanne, Lausanne, Switzerland.
FAU - Thorens, Bernard
AU  - Thorens B
AD  - 3 Center for Integrative Genomics, University of Lausanne, Lausanne, Switzerland.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180321
PL  - United States
TA  - J Cereb Blood Flow Metab
JT  - Journal of cerebral blood flow and metabolism : official journal of the 
      International Society of Cerebral Blood Flow and Metabolism
JID - 8112566
RN  - 0 (Blood Glucose)
RN  - 0 (Glucose Transporter Type 2)
RN  - 0 (Slc2a2 protein, mouse)
SB  - IM
MH  - Animals
MH  - Blood Glucose/*metabolism
MH  - Brain/blood supply/metabolism
MH  - *Cerebrovascular Circulation
MH  - Glucose Transporter Type 2/*metabolism
MH  - Hypoglycemia/blood/*metabolism
MH  - Male
MH  - Mice, Inbred C57BL
PMC - PMC6727137
OTO - NOTNLM
OT  - Glut2
OT  - cerebral blood flow
OT  - glut2 brain cells
OT  - hypoglycemia
OT  - optogenetic
EDAT- 2018/03/22 06:00
MHDA- 2020/05/27 06:00
PMCR- 2020/09/01
CRDT- 2018/03/22 06:00
PHST- 2018/03/22 06:00 [pubmed]
PHST- 2020/05/27 06:00 [medline]
PHST- 2018/03/22 06:00 [entrez]
PHST- 2020/09/01 00:00 [pmc-release]
AID - 10.1177_0271678X18766743 [pii]
AID - 10.1177/0271678X18766743 [doi]
PST - ppublish
SO  - J Cereb Blood Flow Metab. 2019 Sep;39(9):1725-1736. doi: 
      10.1177/0271678X18766743. Epub 2018 Mar 21.