PMID- 29561359
OWN - NLM
STAT- MEDLINE
DCOM- 20190225
LR  - 20221005
IS  - 1872-6623 (Electronic)
IS  - 0304-3959 (Print)
IS  - 0304-3959 (Linking)
VI  - 159
IP  - 7
DP  - 2018 Jul
TI  - Comparative transcriptome profiling of the human and mouse dorsal root ganglia: 
      an RNA-seq-based resource for pain and sensory neuroscience research.
PG  - 1325-1345
LID - 10.1097/j.pain.0000000000001217 [doi]
AB  - Molecular neurobiological insight into human nervous tissues is needed to 
      generate next-generation therapeutics for neurological disorders such as chronic 
      pain. We obtained human dorsal root ganglia (hDRG) samples from organ donors and 
      performed RNA-sequencing (RNA-seq) to study the hDRG transcriptional landscape, 
      systematically comparing it with publicly available data from a variety of human 
      and orthologous mouse tissues, including mouse DRG (mDRG). We characterized the 
      hDRG transcriptional profile in terms of tissue-restricted gene coexpression 
      patterns and putative transcriptional regulators, and formulated an 
      information-theoretic framework to quantify DRG enrichment. Relevant gene 
      families and pathways were also analyzed, including transcription factors, 
      G-protein-coupled receptors, and ion channels. Our analyses reveal an 
      hDRG-enriched protein-coding gene set ( approximately 140), some of which have not been 
      described in the context of DRG or pain signaling. Most of these show conserved 
      enrichment in mDRG and were mined for known drug-gene product interactions. 
      Conserved enrichment of the vast majority of transcription factors suggests that 
      the mDRG is a faithful model system for studying hDRG, because of evolutionarily 
      conserved regulatory programs. Comparison of hDRG and tibial nerve transcriptomes 
      suggests trafficking of neuronal mRNA to axons in adult hDRG, and are consistent 
      with studies of axonal transport in rodent sensory neurons. We present our work 
      as an online, searchable repository 
      (https://www.utdallas.edu/bbs/painneurosciencelab/sensoryomics/drgtxome), 
      creating a valuable resource for the community. Our analyses provide insight into 
      DRG biology for guiding development of novel therapeutics and a blueprint for 
      cross-species transcriptomic analyses.
FAU - Ray, Pradipta
AU  - Ray P
AD  - School of Behavioral and Brain Sciences, The University of Texas at Dallas, 
      Richardson, TX, USA.
AD  - Department of Biological Sciences, The University of Texas at Dallas, Richardson, 
      TX, USA.
FAU - Torck, Andrew
AU  - Torck A
AD  - School of Behavioral and Brain Sciences, The University of Texas at Dallas, 
      Richardson, TX, USA.
FAU - Quigley, Lilyana
AU  - Quigley L
AD  - School of Behavioral and Brain Sciences, The University of Texas at Dallas, 
      Richardson, TX, USA.
FAU - Wangzhou, Andi
AU  - Wangzhou A
AD  - School of Behavioral and Brain Sciences, The University of Texas at Dallas, 
      Richardson, TX, USA.
FAU - Neiman, Matthew
AU  - Neiman M
AD  - School of Behavioral and Brain Sciences, The University of Texas at Dallas, 
      Richardson, TX, USA.
FAU - Rao, Chandranshu
AU  - Rao C
AD  - School of Behavioral and Brain Sciences, The University of Texas at Dallas, 
      Richardson, TX, USA.
FAU - Lam, Tiffany
AU  - Lam T
AD  - School of Behavioral and Brain Sciences, The University of Texas at Dallas, 
      Richardson, TX, USA.
FAU - Kim, Ji-Young
AU  - Kim JY
AD  - School of Behavioral and Brain Sciences, The University of Texas at Dallas, 
      Richardson, TX, USA.
FAU - Kim, Tae Hoon
AU  - Kim TH
AD  - Department of Biological Sciences, The University of Texas at Dallas, Richardson, 
      TX, USA.
FAU - Zhang, Michael Q
AU  - Zhang MQ
AD  - Department of Biological Sciences, The University of Texas at Dallas, Richardson, 
      TX, USA.
FAU - Dussor, Gregory
AU  - Dussor G
AD  - School of Behavioral and Brain Sciences, The University of Texas at Dallas, 
      Richardson, TX, USA.
FAU - Price, Theodore J
AU  - Price TJ
AD  - School of Behavioral and Brain Sciences, The University of Texas at Dallas, 
      Richardson, TX, USA.
LA  - eng
GR  - R01 MH102616/MH/NIMH NIH HHS/United States
GR  - R01 NS098826/NS/NINDS NIH HHS/United States
GR  - R01 NS065926/NS/NINDS NIH HHS/United States
GR  - R01 MH109665/MH/NIMH NIH HHS/United States
GR  - R01 GM102575/GM/NIGMS NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - Pain
JT  - Pain
JID - 7508686
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Ganglia, Spinal/*metabolism
MH  - Gene Expression Profiling
MH  - Humans
MH  - Mice
MH  - Neurosciences
MH  - Pain/genetics/*metabolism
MH  - *Transcriptome
PMC - PMC6008200
MID - NIHMS950896
COIS- Conflict of interest: The authors declare no conflicts of interest
EDAT- 2018/03/22 06:00
MHDA- 2019/02/26 06:00
PMCR- 2019/07/01
CRDT- 2018/03/22 06:00
PHST- 2018/03/22 06:00 [pubmed]
PHST- 2019/02/26 06:00 [medline]
PHST- 2018/03/22 06:00 [entrez]
PHST- 2019/07/01 00:00 [pmc-release]
AID - 00006396-201807000-00016 [pii]
AID - 10.1097/j.pain.0000000000001217 [doi]
PST - ppublish
SO  - Pain. 2018 Jul;159(7):1325-1345. doi: 10.1097/j.pain.0000000000001217.