PMID- 29563184
OWN - NLM
STAT- MEDLINE
DCOM- 20180626
LR  - 20181202
IS  - 1549-5477 (Electronic)
IS  - 0890-9369 (Print)
IS  - 0890-9369 (Linking)
VI  - 32
IP  - 5-6
DP  - 2018 Mar 1
TI  - Stem cell niche-specific Ebf3 maintains the bone marrow cavity.
PG  - 359-372
LID - 10.1101/gad.311068.117 [doi]
AB  - Bone marrow is the tissue filling the space between bone surfaces. Hematopoietic 
      stem cells (HSCs) are maintained by special microenvironments known as niches 
      within bone marrow cavities. Mesenchymal cells, termed CXC chemokine ligand 12 
      (CXCL12)-abundant reticular (CAR) cells or leptin receptor-positive (LepR(+)) 
      cells, are a major cellular component of HSC niches that gives rise to 
      osteoblasts in bone marrow. However, it remains unclear how osteogenesis is 
      prevented in most CAR/LepR(+) cells to maintain HSC niches and marrow cavities. 
      Here, using lineage tracing, we found that the transcription factor early B-cell 
      factor 3 (Ebf3) is preferentially expressed in CAR/LepR(+) cells and that 
      Ebf3-expressing cells are self-renewing mesenchymal stem cells in adult marrow. 
      When Ebf3 is deleted in CAR/LepR(+) cells, HSC niche function is severely 
      impaired, and bone marrow is osteosclerotic with increased bone in aged mice. In 
      mice lacking Ebf1 and Ebf3, CAR/LepR(+) cells exhibiting a normal morphology are 
      abundantly present, but their niche function is markedly impaired with depleted 
      HSCs in infant marrow. Subsequently, the mutants become progressively more 
      osteosclerotic, leading to the complete occlusion of marrow cavities in early 
      adulthood. CAR/LepR(+) cells differentiate into bone-producing cells with reduced 
      HSC niche factor expression in the absence of Ebf1/Ebf3 Thus, HSC cellular niches 
      express Ebf3 that is required to create HSC niches, to inhibit their osteoblast 
      differentiation, and to maintain spaces for HSCs.
CI  - (c) 2018 Seike et al.; Published by Cold Spring Harbor Laboratory Press.
FAU - Seike, Masanari
AU  - Seike M
AD  - Laboratory of Stem Cell Biology and Developmental Immunology, Graduate School of 
      Frontier Biosciences, Graduate School of Medicine, Immunology Frontier Research 
      Center, World Premier International Research Center (WPI), Osaka University, 
      Osaka 565-0871, Japan.
FAU - Omatsu, Yoshiki
AU  - Omatsu Y
AD  - Laboratory of Stem Cell Biology and Developmental Immunology, Graduate School of 
      Frontier Biosciences, Graduate School of Medicine, Immunology Frontier Research 
      Center, World Premier International Research Center (WPI), Osaka University, 
      Osaka 565-0871, Japan.
FAU - Watanabe, Hitomi
AU  - Watanabe H
AD  - Laboratory of Integrative Biological Science and Animal Experiments for 
      Regeneration, Institute for Frontier Life and Medical Sciences, Kyoto University, 
      Kyoto 606-8507, Japan.
FAU - Kondoh, Gen
AU  - Kondoh G
AD  - Laboratory of Integrative Biological Science and Animal Experiments for 
      Regeneration, Institute for Frontier Life and Medical Sciences, Kyoto University, 
      Kyoto 606-8507, Japan.
FAU - Nagasawa, Takashi
AU  - Nagasawa T
AD  - Laboratory of Stem Cell Biology and Developmental Immunology, Graduate School of 
      Frontier Biosciences, Graduate School of Medicine, Immunology Frontier Research 
      Center, World Premier International Research Center (WPI), Osaka University, 
      Osaka 565-0871, Japan.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180321
PL  - United States
TA  - Genes Dev
JT  - Genes & development
JID - 8711660
RN  - 0 (Ebf1 protein, mouse)
RN  - 0 (Ebf3 protein, mouse)
RN  - 0 (Trans-Activators)
RN  - 0 (Transcription Factors)
SB  - IM
CIN - Genes Dev. 2018 Mar 1;32(5-6):324-326. PMID: 29593065
MH  - Age Factors
MH  - Animals
MH  - Bone Marrow/*metabolism/pathology
MH  - Cell Differentiation
MH  - Cell Lineage
MH  - Gene Expression Regulation
MH  - Mesenchymal Stem Cells/cytology/*metabolism/pathology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Osteogenesis/genetics
MH  - Stem Cell Niche
MH  - Trans-Activators/genetics/metabolism
MH  - Transcription Factors/genetics/*metabolism
PMC - PMC5900710
OTO - NOTNLM
OT  - Ebf3
OT  - bone
OT  - hematopoietic stem cell
OT  - mesenchymal stem cell
OT  - stem cell niche
EDAT- 2018/03/23 06:00
MHDA- 2018/06/27 06:00
PMCR- 2018/09/01
CRDT- 2018/03/23 06:00
PHST- 2017/12/22 00:00 [received]
PHST- 2018/02/28 00:00 [accepted]
PHST- 2018/03/23 06:00 [pubmed]
PHST- 2018/06/27 06:00 [medline]
PHST- 2018/03/23 06:00 [entrez]
PHST- 2018/09/01 00:00 [pmc-release]
AID - gad.311068.117 [pii]
AID - 10.1101/gad.311068.117 [doi]
PST - ppublish
SO  - Genes Dev. 2018 Mar 1;32(5-6):359-372. doi: 10.1101/gad.311068.117. Epub 2018 Mar 
      21.